Plant stress analysis: Application of prompt, delayed chlorophyll fluorescence and 820 nm modulated reflectance. Insights from independent experiments

Salvatori, Elisabetta; Fusaro, Lina; Gottardini, E.; Pollastrini, Martina; Goltsev, Vasilij; Strasser, Reto J.; Bussotti, Filippo

doi:10.1016/j.plaphy.2014.11.002

Cited by 81 publications

(52 citation statements)

References 34 publications

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“…Interestingly, these effects were mainly at the PSI level, with a decrease of δ R0 and φ R0 , and a decreased PSI activity and content (ΔV I-P ). Previous studies have already pointed out that PSI is particularly sensitive to heavy metal stress (Wodala et al 2012), as well as to other oxidative stress factors (Bussotti et al 2011;Mereu et al 2011;Salvatori et al 2014). Moreover, it has been recently shown that Pb 2+ is able to interact with PSI at the plastocyanin (PC) level, thus specifically inhibiting the electron transfer process at the PSI donor side (Belatik, Hotchandani and Carpentier 2013).…”

Section: Discussionmentioning

confidence: 99%

Effects of high Zn and Pb concentrations onPhragmites australis(Cav.) Trin. Ex. Steudel: Photosynthetic performance and metal accumulation capacity under controlled conditions

Bernardini

Salvatori

Guerrini

et al. 2015

International Journal of Phytoremediation

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The response of Phragmites australis (Cav.) Trin. Ex. Steudel to zinc (Zn) and lead (Pb) was studied separately in two hydroponic tests, during a three weeks experiment. The effects on ecophysiology and biomass partitioning were evaluated during the metal treatments and at the recovery, and total metal content and accumulation capacity in different plant organs were assessed. Zn and Pb had different effects on the overall measured parameters, highlighting different mechanism of action. In particular, Zn concentration was higher in roots and, being a micronutrient, it was translocated into leaves, producing a reduction of assimilation rate, stomatal conductance (-71.9 and -81.3% respect to the control plant respectively), and a strong down regulation of photosystems functionality both at PSII and PSI level. Otherwise, Pb was accumulated mainly in the more lignified tissue such as rhizomes, with slightly effect on gas exchange. Chlorophyll a fluorescence highlighted that Pb inhibits the electron transfer process at the PSI donor side, without recovery after the removal of the metal stress. Despite these physiological limitations, P. australis showed a high capacity to accumulate both metals, and only slight reduction of biomass, being therefore a suitable species for phytoremediation interventions.

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Section: Discussionmentioning

confidence: 99%

Effects of high Zn and Pb concentrations onPhragmites australis(Cav.) Trin. Ex. Steudel: Photosynthetic performance and metal accumulation capacity under controlled conditions

Bernardini

Salvatori

Guerrini

et al. 2015

International Journal of Phytoremediation

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“…These results suggest that coupled functionality of the two photosystems is essential for an efficient physiological response to oxidative stress (Salvatori et al . ). Indeed, in our study, A max fell slightly even when AOT40 reached values higher than the critical level set for protection of forest species (LRTAP ).…”

Section: Discussionmentioning

confidence: 97%

“…; Salvatori et al . ). While PSII behaviour after O 3 exposure is well known for different plant species and different experimental conditions (Manes et al .…”

Section: Introductionmentioning

confidence: 97%

Early and late adjustments of the photosynthetic traits and stomatal density in Quercus ilex L. grown in an ozone‐enriched environment

et al. 2015

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Quercus ilex L. seedlings were exposed in open-top chambers for one growing season to three levels of ozone (O3 ): charcoal filtered air, non-filtered air supplemented with +30% or +74% ambient air O3 . Key functional parameters related to photosynthetic performance and stomatal density were measured to evaluate the response mechanisms of Q. ilex to chronic O3 exposure, clarifying how ecophysiological traits are modulated during the season in an ozone-enriched environment. Dark respiration showed an early response to O3 exposure, increasing approximately 45% relative to charcoal-filtered air in both O3 enriched treatments. However, at the end of the growing season, maximum rate of assimilation (Amax ) and stomatal conductance (gs ) showed a decline (-13% and -36%, for Amax and gs , respectively) only in plants under higher O3 levels. Photosystem I functionality supported the capacity of Q. ilex to cope with oxidative stress by adjusting the energy flow partitioning inside the photosystems. The response to O3 was also characterised by increased stomatal density in both O3 enriched treatments relative to controls. Our results suggest that in order to improve the reliability of metrics for O3 risk assessment, the seasonal changes in the response of gs and photosynthetic machinery to O3 stress should be considered.

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“…Chlorophyll fluorescence analysis is widely employed in plant stress analysis (Papageorgiou and Govindjee, 2004; Kalaji et al, 2011; Salvatori et al, 2014). The higher plastoquinone pool in transgenic tobacco than WT under stressed conditions indicate better electron transfer from the reaction centers to the quinone pool hence better photosynthesis.…”

Section: Discussionmentioning

confidence: 99%

“…Lower F v /F m in WT under stressed conditions indicated photo-oxidative damage to PSII. Higher chlorophyll fluorescence and F v /F m in transgenic tobacco evident the maintenance of photosynthetic efficiencies under stressed conditions while higher chlorophyll fluorescence and reduced F v /F m in WT plants indicated the structural damage to PSII reaction center (Salvatori et al, 2014). The non-photochemical de-excitation was significantly higher in WT than transgenic tobacco under stressed conditions.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of SbSI-1, A Nuclear Protein from Salicornia brachiata Confers Drought and Salt Stress Tolerance and Maintains Photosynthetic Efficiency in Transgenic Tobacco

et al. 2017

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A novel Salicornia brachiata Salt Inducible (SbSI-1) gene was isolated and overexpressed in tobacco for in planta functional validation subjected to drought and salt stress. SbSI-1 is a nuclear protein. The transgenic tobacco overexpressing SbSI-1 gene exhibited better seed germination, growth performances, pigment contents, cell viability, starch accumulation, and tolerance index under drought and salt stress. Overexpression of SbSI-1 gene alleviated the build-up of reactive oxygen species (ROS) and curtailed the ROS-induced oxidative damages thus improved the physiological health of transgenic tobacco under stressed conditions. The higher activities of antioxidant enzymes, lower accumulation of ROS, higher membrane stability, relative water content, and polyphenol contents indicated the better survival of the transgenic tobacco than wild-type (WT) tobacco under stressed conditions. Transgenic tobacco had a higher net photosynthetic rate, PSII operating efficiency, and performance index under drought and salt stress. Higher accumulation of compatible solutes and K+/Na+ ratio in transgenic tobacco than WT showed the better osmotic and redox homeostasis under stressed conditions. The up-regulation of genes encoding antioxidant enzymes (NtSOD, NtAPX, and NtCAT) and transcription factors (NtDREB2 and NtAP2) in transgenic tobacco under stressed conditions showed the role of SbSI-1 in ROS alleviation and involvement of this gene in abiotic stress tolerance. Multivariate data analysis exhibited statistical distinction among growth responses, physiological health, osmotic adjustment, and photosynthetic responses of WT and transgenic tobacco under stressed conditions. The overexpression of SbSI-1 gene curtailed the ROS-induced oxidative damages and maintained the osmotic homeostasis under stress conditions thus improved physiological health and photosynthetic efficiencies of the transgenic tobacco overexpressing SbSI-1 gene.

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Plant stress analysis: Application of prompt, delayed chlorophyll fluorescence and 820 nm modulated reflectance. Insights from independent experiments

Cited by 81 publications

References 34 publications

Effects of high Zn and Pb concentrations onPhragmites australis(Cav.) Trin. Ex. Steudel: Photosynthetic performance and metal accumulation capacity under controlled conditions

Effects of high Zn and Pb concentrations onPhragmites australis(Cav.) Trin. Ex. Steudel: Photosynthetic performance and metal accumulation capacity under controlled conditions

Early and late adjustments of the photosynthetic traits and stomatal density in Quercus ilex L. grown in an ozone‐enriched environment

Overexpression of SbSI-1, A Nuclear Protein from Salicornia brachiata Confers Drought and Salt Stress Tolerance and Maintains Photosynthetic Efficiency in Transgenic Tobacco

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