Plant regeneration from in vitro leaves of mature black cherry (Prunus serotina)

Liu, Xiaomei; Pijut, Paula M.

doi:10.1007/s11240-008-9393-x

Cited by 53 publications

(46 citation statements)

References 28 publications

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“…There are also no quantitative studies that examine the relation between the age of the explant tissue, or the age of the mother plant, and the level of contamination. Separately, the correlation between the age of the mother plant and regeneration efficiency has been reported in other species in which juvenile tissues generally resulted in better regeneration than tissues from adult mother plants or were less recalcitrant to regeneration (Liu and Pijut 2008, Cardoso and Habermann 2014, Lema-Rumińska and Kulus 2014. In addition, we observed that in most Dendrobium micropropagation studies that employed somatic tissues as explants, young shoots (0.5 to 12 cm long) were the main explant used rather than mature shoots with leaves or pseudobulbs (Tab.…”

Section: In Vivo Conditions Of Donor Plants and Explant Choicesupporting

confidence: 67%

Tissue disinfection for preparation of Dendrobium in vitro culture

Silva¹,

Winarto²,

Dobránszki

et al. 2016

Folia Horticulturae

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Establishing an aseptic in vitro culture for Dendrobium, or for any plant in fact, is the most important step towards developing an effective in vitro tissue culture including micropropagation protocol. Success in initial aseptic culture will contribute to the successful production of in vitro cultures that may involve the initiation or formation of callus and/or protocorm-like bodies (PLBs), the induction, regeneration or multiplication of shoots, and the preparation and proliferation of plantlets suitable for acclimatization. The initiation of an aseptic culture is closely related to the appropriate selection of an explant source and its preparation, including its (in vivo) pre-treatment if necessary and subsequent disinfection procedures. Care in the choice of explant and the application of an appropriate disinfection protocol can successfully reduce, or eliminate, contamination in in vitro cultures while reducing the negative impact on plant tissues and plantlet regeneration. Many unique aseptic culture procedures for Dendrobium genus have been reported in the literature, very often specific to particular tissues or genotypes, and this review not only highlights the details of such protocols, but also provides practical advice for novice -and even seasoned -orchidologists who wish to research Dendrobium in vitro, although it is cautioned that there is currently no universal aseptic culture procedure that can be applied to all conditions, all explants or all genotypes.

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Section: In Vivo Conditions Of Donor Plants and Explant Choicesupporting

confidence: 67%

Tissue disinfection for preparation of Dendrobium in vitro culture

Silva¹,

Winarto²,

Dobránszki

et al. 2016

Folia Horticulturae

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“…Similarly maximum number of shoots was achieved from leaf section callus of Hyptis suaveolens [34] on MS medium containing 0.5 mg/L BA and 0.5 mg/L GA 3 . In the present investigation, 71.6% of the organogenic calli derived through leaf section explants on MS medium supplemented with 1.0 mg/L TDZ and 0.25 mg/L NAA produced an average of 2.1 shoots per callus explant which was consistent with the previous reports such as, Saussurea obvallata [14] [39], Aechmea fasciata [40] and Echinacea purpurea [26]. Ghimire et al [41] obtained highest mean number (10.65) of shoots per explant from in vitro leaves of Drymaria cordata on medium containing 1.0 mg/L BA and 0.1 mg/L NAA.…”

Section: Discussionsupporting

confidence: 92%

<i>In Vitro</i> Organogenesis of <i>Quisqualis indica </i>Linn.

Mandal¹,

Laxminarayana²

2012

AJPS

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Shoot organogenesis and plant regeneration were achieved on callus derived from leaf section and stem base explants of Quisqualis indica (Combretaceae). In vitro cultures were established using nodal segments obtained from mature field-grown shrubby plants. For the development of optimized protocol, different types and concentrations of plant growth regulators were used to induce adventitious shoot regeneration via callus from leaf section and one-node stem base explants obtained from in vitro regenerated micro shoots and direct field-grown newly flush-off shoots. The TDZ was considered to be the best among the cytokinins (6-benzyladenine (BA), 6-(-, dimethylallyamino purine) (2-iP) and thidiazuron (TDZ) added to the Murashige and Skoog's medium (MS) for adventitious shoot productions. A combination of 1.0 mg/L TDZ and 0.5 mg/L GA 3 was most effective in stimulating callus induction and adventitious shoot regeneration from the leaf section derived calli with an average of 6 shoots per callus explant and an average of 8 shoots per callus explant originated from one-node stem base explants. In vitro raised shoots were sub-cultured on MS medium supplemented with 1.0 mg/L BA and 0.5 mg/L GA 3 for further shoot growth. Maximum rooting of in vitro regenerated shoots was obtained on MS medium supplemented with either 0.5 mg/L indole-3-acetic acid (IAA) or indole-3-butyric acid (IBA) individually or a combination of 0.5 mg/L IAA and 0.5 mg/L IBA. Plantlets raised in vitro were acclimatized and subsequently transferred to experimental field.

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“…A morfogênese, caracterizada pela emergência e formação de novos órgãos, resulta da interação de processos de indução, competência, determinação e diferenciação celular, os quais são influenciados pelo meio de cultivo, pelo genótipo, pelo tipo e pelas condições fisiológicas dos explantes, bem como pelas condições físicas do cultivo in vitro (LIU & PIJUT, 2008).…”

Section: Introductionunclassified