“…As is well known, the growth and development of plant need water, mineral and organic matter, and also are regulated by plant growth substance which is very critical to growth and differentiation of cell and tissue, induction and formation of bud and root (Melissa et al, 2006;Randy et al, 2009;Duan et al, 2013;Dragicevic et al, 2013;Gerszberg et al, 2015). At present, 2,4-dichlorophenoxy acetic acid (2,4-D), Naphthylacetic acid (NAA), 6-benzylaminopurine (6-BA) and other plant growth substances are often applied in plant tissue culture, in which NAA, 2,4-D and Indole butyric acid (IBA) belong to auxin and mainly play a role in callus formation, embryoid regeneration, and so on, yet 6-BA, Kinetin (KT) and Zeatin (ZT) 7 belong to cytokinin, and principally act on stimulants of cell division and induction of bud differentiation.…”