Plant phosphoinositide-dependent phospholipases C: Variations around a canonical theme

Pokotylo, Igor; Kolesnikov, Yaroslav S.; Kravets, V. S.; Zachowski, Alain; Ruelland, Éric

doi:10.1016/j.biochi.2013.07.004

Cited by 115 publications

(84 citation statements)

References 193 publications

(280 reference statements)

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“…During the preparation of this manuscript, a comprehensive review was published, 37 describing different features of plant PI-PLCs including expression patterns of different isoforms in conjunction with biotic and abiotic challenges in different plant species. Of the various isoforms known, the PLC2 isoform, used in this study, although constitutively expressed in Arabidopsis, is not significantly induced by low temperature or biotic stress conditions.…”

Section: Discussionmentioning

confidence: 99%

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Metabolomic shifts inBrassica napuslines with enhancedBnPLC2expression impact their response to low temperature stress and plant pathogens

et al. 2014

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Phosphatidylinositol-specific phospholipase C2 (PLC2) is a signaling enzyme with hydrolytic activity against membrane-bound phosphoinositides. It catalyzes the cleavage of phosphatidylinositol(4,5)bisphosphate (PtdIns(4,5) P 2 ) into two initial second messengers, myo-inositol-1,4,5-trisphosphate (InsP 3 ) and diacylglycerol (DAG). The former, as well as its fully phosphorylated derivative, myo-inositol-1,2,3,4,5,6-hexakisphosphate (InsP 6 ), play a major role in calcium signaling events within the cell, while DAG may be used in the regeneration of phospholipids or as a precursor for phosphatidic acid (PA) biosynthesis, an important signaling molecule involved in both biotic and abiotic types of stress tolerance. Overexpression of the gene for Brassica napus phospholipase C2 (BnPLC2) in Brassica napus has been shown to enhance drought tolerance, modulate multiple genes involved in different processes and favorably affect hormonal levels in different tissues. We, therefore, undertook the current study with a view to examining, at the metabolome level, its effect on both abiotic (low temperature) and biotic (stem white rot disease) types of stress in canola. Thus, while transgenic plants exhibited a significant rise in maltose levels and a concomitant elevation in some unsaturated free fatty acids (FFAs), glycerol, and glycerol 3-phosphate under subzero temperatures, they accumulated high levels of raffinose, stachyose and other sugars as well as some flavonoids under acclimatization conditions. Collectively, overexpression of BnPLC2 appears to have triggered different metabolite patterns consistent with its abiotic and, to a limited extent, biotic stress tolerance phenotypes.

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Section: Discussionmentioning

confidence: 99%

“…Interestingly, while the same isoform is shown to be upregulated by cold and downregulated by biotic stress in wheat, its expression follows an opposite trend in rice. 37 What is more, in B. napus, unlike Arabidopsis, this isoform is significantly induced by cold, 12 implying the possibility of species-specific expression regulation.…”

Section: Discussionmentioning

confidence: 99%

Metabolomic shifts inBrassica napuslines with enhancedBnPLC2expression impact their response to low temperature stress and plant pathogens

et al. 2014

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“…These second messengers then activate protein kinase C (PKC) and the ER-localised IP 3 receptor, respectively, in animal cells [1,2]. However, although the PI-PLC signaling cascade is present in plants [5][6][7], genes encoding PKC and the IP 3 receptor have not been found in terrestrial plant genomes, suggesting differences in second messenger systems between animals and plants. To date, the genomes of a variety of unicellular and multicellular algae have been sequenced [8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23] as shown in (Table 1).…”

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confidence: 99%

“…To date, PA and IP 6 are thought to act as major second messengers in plant cells [7,54], although the function of IP 3 as a second messenger in plants has not been ruled out [42,47]. For instance, Munnik and Vermeer [54] have proposed that IP 6 , which is rapidly converted from IP 3 , is a major second messenger involved in abscisic acid-dependent inhibition of stomatal opening.…”

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Comparative Genomic View of The Inositol-1,4,5-Trisphosphate Receptor in Plants

Mikami¹

2014

J Plant Biochem Physiol

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Inositol-1,4,5-trisphosphate [Ins(1,4,5)P 3 , IP 3 ] is a second messenger involved in transient release of Ca 2+ from the ER that activates cytosolic Ca 2+ signalling cascades in response to extracellular and intracellular stimuli [1,2]. Phosphatidylinositol-4,5-bisphosphate is cleaved by phosphoinositide-specific phospholipase C (PI-PLC) into the second messengers diacylglycerol (DAG) and IP 3 [3,4]. These second messengers then activate protein kinase C (PKC) and the ER-localised IP 3 receptor, respectively, in animal cells [1,2]. However, although the PI-PLC signaling cascade is present in plants [5][6][7], genes encoding PKC and the IP 3 receptor have not been found in terrestrial plant genomes, suggesting differences in second messenger systems between animals and plants. To date, the genomes of a variety of unicellular and multicellular algae have been sequenced [8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23] as shown in (Table 1). In addition, large-scale EST information for the red seaweeds Porphyra umbilicalis and Porphyra purpurea has been accumulated [24][25][26]. Such rich gene information enables us to identify the genes encoding IP 3 receptor gene homologues in algae to hypothesize the evolutionary route of the loss of the IP 3 gene in plant lineages.The origin of the IP 3 receptor-dependent transient Ca 2+ release system predates the divergence of animals and fungi [27,28]. Indeed, homologues of genes encoding the IP 3 receptor have been identified in protozoa such as the choanoflagellate Monosiga brevicollis [29], the myxomycete Dictyostelium discoideum [30], the ciliate Paramecium tetraurelia [31], and the parasite Trypanosoma brucei [32]. Thus, it is plausible that an ancient eukaryotic cell containing an IP 3 receptor gene was the target of endosymbiosis with an ancient cyanobacterium to produce plant cells, after which the IP 3 gene was lost from plant lineages. At present, IP 3 receptor homologues have been found in green algae, such as Chlamydomonas reinhardtii [10] and Volvox carteri [33,34], and in heterokont algae including Aureococcus anophagefferrens [21] and Ectocarpus siliculosus [22], but have not been identified in red algae or streptophytes (land plants and charophytic algae) (Figure 1). These findings have led to proposals that the IP 3 receptor gene homologue was lost on multiple occasions during plant evolution. Because an ancestor of both green and red photosynthetic algal cells appeared after the primary endosymbiosis of a cyanobacterium into an ancient non-photosynthetic eukaryotic cell [35], the IP 3 receptor homologue was probably lost from lineages of red algae and green algae except for Volvocales (Figure 1). In fact, the genomes of unicellular Aureococcus anophagefferrens and multicellular Ectocarpus siliculosus carry an IP 3 receptor gene homologue (Figure 1). Because both photosynthetic algae arose from secondary endosymbiosis of a red algal cell into an ancient non-photosynthetic eukaryotic cell [35], it appears that red algae subsequently lost the IP...

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“…This lipid can be phosphorylated into phosphatidic acid by diacylglycerol-kinases (DGK). 6 The PI-PLC/DGK pathway is active in nonstimulated Arabidopsis cells or plants. 7,8 We recently showed that this basal activity negatively regulated the expression of DREB2A, DREB2B, DREB2C, DREB2E, and DREB2H genes.…”

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confidence: 99%

The phosphoinositide dependent-phospholipase C pathway differentially controls the basal expression ofDREB1andDREB2genes

Ruelland¹,

Djafi²,

Zachowski³

2013

Plant Signaling & Behavior

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W e recently showed that-in Arabidopsis thaliana suspension cells-phosphoinositide dependent-phospholipase C (PI-PLC) and diacylglycerol kinase (DGK) negatively regulated the basal expression of most DREB2 genes. DREB2 genes encode transcription factors that bind to Drought Responsive Elements (DRE). Those elements are also bound by DREB1 factors. While DREB2 factors are mostly involved in drought and heat responses, DREB1s are induced in the response to chilling. We here show that the pharmacological inhibition of PI-PLC or DGK leads to the basal induction of DREB1 genes. However, the induction is much less marked for the DREB1 genes than that of DREB2A, a member of the DREB2 family. This illustrates that DREB1 and DREB2 genes, while having the same targets, are not submitted to the same transcription regulation, and that lipid signaling might in part explain these differences in the regulation of the DREB genes.

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Plant phosphoinositide-dependent phospholipases C: Variations around a canonical theme

Cited by 115 publications

References 193 publications

Metabolomic shifts inBrassica napuslines with enhancedBnPLC2expression impact their response to low temperature stress and plant pathogens

Metabolomic shifts inBrassica napuslines with enhancedBnPLC2expression impact their response to low temperature stress and plant pathogens

Comparative Genomic View of The Inositol-1,4,5-Trisphosphate Receptor in Plants

The phosphoinositide dependent-phospholipase C pathway differentially controls the basal expression ofDREB1andDREB2genes

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