Plant Hormones and Initiation of Legume Nodulation and Arbuscular Mycorrhization

Mukherjee, Arijit

doi:10.1002/9780470959404.ch16

Cited by 11 publications

(23 citation statements)

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“…For instance, the interaction between auxin and cytokinin is especially significant to control the formation and maintenance of meristems (Su et al, 2011). This interplay between auxin and cytokinin is also important for lateral root and nodule formation (Mathesius, 2008; Ding and Oldroyd, 2009; Desbrosses and Stougaard, 2011; Mukherjee and Ané, 2011b; Ryu et al, 2012; Bensmihen, 2015). Altering the auxin to cytokinin balance specifies whether root cells divide in the pericycle or in the cortex (Mathesius, 2008).…”

Section: Discussionmentioning

confidence: 99%

“…), MtN3 (LOC_Os01g36070.1, LOC_Os01g50460.1, and LOC_Os01g12130.1), NOD-26-like intrinsic proteins (LOC_Os02g13870.1, LOC_Os02g51110.1, and LOC_Os06g12310.1), and Major Facilitator Superfamily (e.g., LOC_Os03g11900.1, LOC_Os04g42130.1, LOC_Os07g03910.1, etc.). Nodulin-encoding genes were first defined as legume genes that are specifically expressed during legume–rhizobia symbioses (Oldroyd and Downie, 2008; Mukherjee and Ané, 2011b). Interestingly, homologs of nodulin genes have been identified in several non-legumes suggesting a possible role of these genes in plant physiology (Denance et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…Several studies have shown that plant hormones play key roles throughout nodule organogenesis in the integration of developmental and environmental signaling cues during nodule development (Ding and Oldroyd, 2009; Mukherjee and Ané, 2011b; Ryu et al, 2012; Bensmihen, 2015). For instance, the interplay of auxin and cytokinin plays a major role in nodule organogenesis (Suzaki et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…Surprisingly, genetic analysis of pathways controlling initiation of nodule development and lateral root formation has not identified an obvious overlap (Desbrosses and Stougaard, 2011). Also, very few legume mutants have been identified that directly affect hormonal pathways (Desbrosses and Stougaard, 2011; Mukherjee and Ané, 2011b), possibly due to genetic redundancy. One option to elucidate the genetic mechanism in hormone-dependent nodule organogenesis is to study NLS formation.…”

Section: Introductionmentioning

confidence: 99%

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A Developmental and Molecular View of Formation of Auxin-Induced Nodule-Like Structures in Land Plants

et al. 2016

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Several studies have shown that plant hormones play important roles during legume–rhizobia symbiosis. For instance, auxins induce the formation of nodule-like structures (NLSs) on legume roots in the absence of rhizobia. Furthermore, these NLS can be colonized by nitrogen-fixing bacteria, which favor nitrogen fixation compared to regular roots and subsequently increase plant yield. Interestingly, auxin also induces similar NLS in cereal roots. While several genetic studies have identified plant genes controlling NLS formation in legumes, no studies have investigated the genes involved in NLS formation in cereals. In this study, first we established an efficient experimental system to induce NLS in rice roots, using auxin, 2,4-D, consistently at a high frequency (>90%). We were able to induce NLS at a high frequency in Medicago truncatula under similar conditions. NLS were characterized by a broad base, a diffuse meristem, and increased cell differentiation in the vasculature. Interestingly, NLS formation appeared very similar in both rice and Medicago, suggesting a similar developmental program. We show that NLS formation in both rice and Medicago occurs downstream of the common symbiotic pathway. Furthermore, NLS formation occurs downstream of cytokinin-induced step(s). We performed a comprehensive RNA sequencing experiment to identify genes differentially expressed during NLS formation in rice and identified several promising genes for control of NLS based on their biological and molecular functions. We validated the expression patterns of several genes using reverse transcription polymerase chain reaction and show varied expression patterns of these genes during different stages of NLS formation. Finally, we show that NLS induced on rice roots under these conditions can be colonized by nitrogen-fixing bacteria, Azorhizobium caulinodans.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A Developmental and Molecular View of Formation of Auxin-Induced Nodule-Like Structures in Land Plants

et al. 2016

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“…Plant hormones such as auxins and cytokinins play key roles during different stages of nodule organogenesis in legume roots (Bensmihen 2015; Ding and Oldroyd 2009; Mukherjee and Ané 2011; Ryu et al 2012). Genetic studies have improved our understanding of how hormones regulate this symbiosis.…”

Section: Introductionmentioning

confidence: 99%

Comparative transcriptome analysis provides key insights into gene expression pattern during the formation of nodule-like structures in Brachypodium

Thomas

Bowman

Vega

et al. 2018

Funct Integr Genomics

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Auxins can induce the formation of nodule-like structures (NLS) in plant roots even in the absence of rhizobia and nitrogen-fixing bacteria can colonize these structures. Interestingly, NLS can be induced in roots of both legumes and non-legumes. However, our understanding of NLS formation in non-legumes at a molecular level is limited. This study aims to investigate NLS formation at a developmental and molecular level in Brachypodium distachyon. We treated Brachypodium roots with the synthetic auxin, 2,4-D, to induce NLS at a high frequency (> 80%) under controlled conditions. A broad base and a diffuse meristem characterized these structures. Next, we performed a comprehensive RNA-sequencing experiment to identify differentially expressed genes (DEGs) in Brachypodium roots during NLS formation. We identified 618 DEGs; several of which are promising candidates for control of NLS based on their biological and molecular functions. We validated the expression pattern of several genes via RT-PCR. Next, we compared the expression profile of Brachypodium roots with rice roots during NLS formation. We identified 76 single-copy ortholog pairs in rice and Brachypodium that are both differentially expressed during this process. Some of these genes are involved in auxin signaling, root development, and legume-rhizobia symbiosis. We established an experimental system to study NLS formation in Brachypodium at a developmental and genetic level, and used RNA-sequencing analysis to understand the molecular mechanisms controlling this root organogenesis program. Furthermore, our comparative transcriptome analysis in Brachypodium and rice identified a key set of genes for further investigating this genetic pathway in grasses.

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Legumes and Nitrogen Fixation: Physiological, Molecular, Evolutionary Perspectives, and Applications

Venkateshwaran

2011

The Molecular and Physiological Basis of Nutrient Use Efficiency in Crops

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Plant Hormones and Initiation of Legume Nodulation and Arbuscular Mycorrhization

Cited by 11 publications

References 308 publications

A Developmental and Molecular View of Formation of Auxin-Induced Nodule-Like Structures in Land Plants

A Developmental and Molecular View of Formation of Auxin-Induced Nodule-Like Structures in Land Plants

Comparative transcriptome analysis provides key insights into gene expression pattern during the formation of nodule-like structures in Brachypodium

Legumes and Nitrogen Fixation: Physiological, Molecular, Evolutionary Perspectives, and Applications

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