Plant growth promoting and antagonistic properties of endophytic bacteria isolated from domestic apple

Miliūtė, Inga; Buzaitė, Odeta; Gelvonauskienė, D.; Sasnauskas, A.; Stanys, V.; Baniulis, Danas

doi:10.13080/z-a.2016.103.010

Cited by 25 publications

(26 citation statements)

References 29 publications

(28 reference statements)

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“…The involved mechanisms for this include production of cell-wall degrading enzymes (e.g., chitinase and β-1,3-glucanase) and antimicrobial compounds, lowering endogenous stress-related ET in plants, induction of induced systemic resistance (ISR) in host plants, quenching the quorum sensing (QS) of phytopathogens and competition for niche and resources ( Compant et al, 2010 ; Glick, 2014 ; Santoyo et al, 2016 ). A single endophytic bacterial strain or bacterial community may have more than one of these plant growth-promoting traits (PGPTs) ( Rolli et al, 2015 ; Tsurumaru et al, 2015 ; Miliute et al, 2016 ) ( Figure 5 ). Bacterial strains with plant growth-promoting functions continue to be discovered but as of yet a clear path to developing PGPTs for agricultural purposes has not been developed ( Dey et al, 2014 ).…”

Section: Plant Growth-promoting Traitsmentioning

confidence: 99%

Inner Plant Values: Diversity, Colonization and Benefits from Endophytic Bacteria

Liu

Carvalhais

Crawford³

et al. 2017

Front. Microbiol.

526

342

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One of the most exciting scientific advances in recent decades has been the realization that the diverse and immensely active microbial communities are not only ‘passengers’ with plants, but instead play an important role in plant growth, development and resistance to biotic and abiotic stresses. A picture is emerging where plant roots act as ‘gatekeepers’ to screen soil bacteria from the rhizosphere and rhizoplane. This typically results in root endophytic microbiome dominated by Proteobacteria, Actinobacteria and to a lesser extent Bacteroidetes and Firmicutes, but Acidobacteria and Gemmatimonadetes being almost depleted. A synthesis of available data suggest that motility, plant cell-wall degradation ability and reactive oxygen species scavenging seem to be crucial traits for successful endophytic colonization and establishment of bacteria. Recent studies provide solid evidence that these bacteria serve host functions such as improving of plant nutrients through acquisition of nutrients from soil and nitrogen fixation in leaves. Additionally, some endophytes can engage ‘priming’ plants which elicit a faster and stronger plant defense once pathogens attack. Due to these plant growth-promoting effects, endophytic bacteria are being widely explored for their use in the improvement of crop performance. Updating the insights into the mechanism of endophytic bacterial colonization and interactions with plants is an important step in potentially manipulating endophytic bacteria/microbiome for viable strategies to improve agricultural production.

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Section: Plant Growth-promoting Traitsmentioning

confidence: 99%

Inner Plant Values: Diversity, Colonization and Benefits from Endophytic Bacteria

Liu

Carvalhais

Crawford³

et al. 2017

Front. Microbiol.

526

342

View full text Add to dashboard Cite

show abstract

“…In a previous study, we isolated 38 endophytic bacteria from apple buds of cultivars “Gala,” “Golden Delicious,” and “Orlovim” grown under field conditions ( Miliute et al, 2016 ). Biochemical analysis revealed several traits that are important for plant growth stimulation, including nitrogen fixation and the production of indole-3-acetic acid and siderophores that could have important implications on plant growth under in vitro conditions.…”

Section: Introductionmentioning

confidence: 99%

Endophytic Bacillus and Pseudomonas spp. Modulate Apple Shoot Growth, Cellular Redox Balance, and Protein Expression Under in Vitro Conditions

et al. 2018

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Interactions between host plants and endophytic microorganisms play an important role in plant responses to pathogens and environmental stresses and have potential applications for plant stress management under in vitro conditions. We assessed the effect of endophytic bacteria on the growth and proliferation of domestic apple cv. Gala shoots in vitro. Further, a model apple cell suspension system was used to examine molecular events and protein expression patterns at an early stage of plant–endophyte interaction. Among the seven strains used in the study, Bacillus spp. strains Da_1, Da_4, and Da_5 and the Pseudomonas fluorescens strain Ga_1 promoted shoot growth and auxiliary shoot proliferation. In contrast, Bacillus sp. strain Oa_4, P. fluorescens strain Ga_3 and P. orientalis strain G_12 inhibited shoot development. In the cell suspension, the effects of the association between endophytic bacteria and plant cells were specific to each strain. Modulation of the cellular redox balance was monitored in the apple cells using a 2′,7′-dichlorodihydrofluorescein diacetate (H2DCFDA) probe, and strain-specific effects were observed that correlated with the in vitro shoot development results. Proteomic analysis revealed differences in protein expressions in apple cells co-cultivated with different Bacillus spp. strains that had contrasting effects on cellular redox balance and shoot development. The Bacillus sp. strain Da_4, which enhanced shoot development and oxidation of H2DCFDA, induced differential expression of proteins that are mainly involved in the defense response and regulation of oxidative stress. Meanwhile, treatment with Bacillus sp. strain Oa_4 led to strong upregulation of PLAT1, HSC70-1 and several other proteins involved in protein metabolism and cell development. Taken together, the results suggest that different cell signaling and response events at the early stage of the plant–endophyte interaction may be important for strain-dependent regulation of cellular redox balance and development of shoot phenotype.

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“…Endophytic bacterial communities and even a single endophytic bacterial strain can have multiple PGPTs (Miliute et al, 2016;Rolli et al, 2015;Tsurumaru et al, 2015). Generally, growth stimulation by endophytic bacteria can be a consequence of phytohormone production, elicitation of plant priming conditions, suppression of phytopathogens and/or improvement of plant nutrition (Fig.…”

Section: Plant Growth-promoting Traitsmentioning

confidence: 99%

Effects of strategic tillage and plant hormone treatments on wheat-associated microbial communities

Liu¹

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The main aim of this thesis was to characterise microbial properties of microbiomes associated with wheat (Triticum aestivum) soils and wheat plants. In particular my objective was to better understand the effects on microbial communities following strategic tillage (ST) in wheat field soils and the activation of plant defence pathways in wheat plants. Throughout the thesis, multiple culture-independent methods, especially next-generation sequencing of 16S rRNA gene were used to profile soil microbial communities. Over half of the arable land in the northern grains region of Australia is managed using no-till (NT), a farming method which has improved crop yields and soil quality while reducing the input and labour costs. However, in recent years, concerns have arisen among farmers over the weed control in continuous NT systems. Strategic tillage has been touted as a potential solution, in particular for the severe weed infestations of long-term NT. Nevertheless, there is little information on the influence of ST on the microbial properties of Australian NT soils.In the present study, ST applications and soil sampling were performed in Moonie, Moree and Condamine during the fallow period in eastern Australia. These sites were chosen based on their long history of repeated wheat cultivation under NT farming practices and their different soil types.Overall, results show that in the Moonie trial on a Calcisol, one-time ST with either chisel or offset disc did not significantly influence the composition of soil bacterial communities when measured 13 months after tillage. However, relative to the NT, chisel tillage led to significant increases in microbial biomass carbon (MBC), abundances of Alphaproteobacteria, Bacteroidetes and Firmicutes as well as the utilisation of D+cellubiose and mannitol at 0-10 cm depth. In the Moree trial on a grey Vertosol, ST with different timing and implement demonstrated great potential in weed control and did not influence wheat yield and soil physicochemical and biological properties in the short-term. In the Condamine site on a Solonetz soil, one-or two-time chisel tillage did not influence soil MBC, total microbial enzymatic activity (MEA) or utilisation of C substrates.Likewise, ST did not change the soil microbial community structure and the abundance of genes encoding enzymes involved in key steps of C and N reactions. However, one-time chisel increased relative abundance of Acidobacteria RB41 and Acidobacteria iii1-15, and two-time chisel slightly increased the average C utilisation, at 10-20 cm depth. Overall, this thesis suggests that ST does not cause major impacts on soil properties of long-term NT and could be potentially used to address the long-term NT-associated issues without impacting overall soil properties.Recent reports demonstrate the importance of microbiomes associated with plants and their soil they are cultivated in. Beneficial microbes can significantly increase crop yields and provide biocontrol functions against plant pathogens, but prior to this stu...

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Plant growth promoting and antagonistic properties of endophytic bacteria isolated from domestic apple

Cited by 25 publications

References 29 publications

Inner Plant Values: Diversity, Colonization and Benefits from Endophytic Bacteria

Inner Plant Values: Diversity, Colonization and Benefits from Endophytic Bacteria

Endophytic Bacillus and Pseudomonas spp. Modulate Apple Shoot Growth, Cellular Redox Balance, and Protein Expression Under in Vitro Conditions

Effects of strategic tillage and plant hormone treatments on wheat-associated microbial communities

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