Plant-associated microbial communities converge in fermentative hydrogen production and form a core microbiome

Ayala-Campos, Olga R.; Sánchez, Arturo; Rebollar, Eria A.; Valdez‐Vazquez, Idania

doi:10.1016/j.ijhydene.2022.04.155

Cited by 9 publications

(8 citation statements)

References 55 publications

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“…Clostridium butyricum has outranked other species for giving a better biohydrogen production rate from glucose (3.90 mL H2/g glucose at 10 g/L of glucose) [144]. Most recently, Campos et al [146] utilized four lignocellulosic plant-based microbial communities, i.e., Clostridium, Lactobacillus, Enterobacter, and Pichia (fungus), through a consolidated bioprocessing approach. In the study, at a feeding rate of 10 g/L.…”

Section: Inoculummentioning

confidence: 99%

Biohydrogen production through dark fermentation from waste biomass: Current status and future perspectives on biorefinery development

D’Silva

Khan

Kumar

et al. 2023

Fuel

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Section: Inoculummentioning

confidence: 99%

Biohydrogen production through dark fermentation from waste biomass: Current status and future perspectives on biorefinery development

D’Silva

Khan

Kumar

et al. 2023

Fuel

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“…Alternatively, a consolidated bioprocess (CBP) could be applied due to its advantage of combining three processes occurring in the same reactor: production of saccharolytic enzymes, hydrolysis of the polysaccharides, and pentose and hexose fermentation (Dudek et al 2021 ). Indigenous microbiota in lignocelluloses are a promising biocatalyst to perform the CBP for hydrogen production (Ayala-Campos et al 2022 ). Recent research has evidenced how different indigenous microbiota converged into a core microbiome formed by Clostridium and Lactobacillus , genera that were positively associated with hydrogen production.…”

Section: Introductionmentioning

confidence: 99%

“…The genus Enterococcus was probably the first lactic acid bacteria reported in reactors that produced hydrogen via CBP from untreated wheat straw thanks to their xylanolytic activity (Valdez-Vazquez et al 2015 , 2017 ). On the other hand, the lactic acid bacterium Lactobacillus has also been reported in hydrogen-producing reactors via CBP (Ayala-Campos et al 2022 ; Pérez-Rangel et al 2023 ). These two LAB seem to establish different interactions with hydrogen producers; for example, Pérez-Rangel et al ( 2022 ) reported antagonism between Lactobacillus – Enterococcus and Enterococcus – Clostridium , but not between Lactobacillus – Clostridium .…”

Section: Introductionmentioning

confidence: 99%

Microbial activity of lactic acid bacteria and hydrogen producers mediated by pH and total solids during the consolidated bioprocessing of agave bagasse

Dudek,

Guzmán,

Valdez-Vazquez

2024

World J Microbiol Biotechnol

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Lactic acid bacteria (LAB) coexist with Clostridium spp. in hydrogen production processes from complex substrates; however, the role of LAB is still unclear. This study analyzed the fermentation products in a wide range of initial pH (pHi, 5.5–6.9) and total solids (TS%, 8–22%) to determine the activity of these two microbial groups over time (from 24 to 120 h). Agave bagasse served as the feedstock for hydrogen production via consolidated bioprocess (CBP), while the inoculum source was the indigenous mature microbiota. In the early stage of the CBP, hydrogen production from lactic acid occurred only at pHi ≥ 6.0 (ρ = 0.0004) with no effect of TS%; lactic acid accumulated below this pHi value. In this stage, lactic acid production positively correlated with a first cluster of LAB represented by Paucilactobacillus (r = 0.64) and Bacillus (r = 0.81). After 72 h, hydrogen production positively correlated with a second group of LAB led by Enterococcus (r = 0.71) together with the hydrogen producer Clostridium sensu stricto 1 (r = 0.8) and the acetogen Syntrophococcus (r = 0.52) with the influence of TS% (ρ < 0.0001). A further experiment showed that buffering the pH to 6.5 increased and lengthened the lactic acid production, doubling the hydrogen production from 20 to 41 mL H2/gTSadded. This study confirmed the prevalence of distinct groups of LAB over time, whose microbial activity promoted different routes of hydrogen production.

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“…Microbial communities have been very successful in producing biofuels such as methane, hydrogen, and ethanol directly from lignocellulose (Ayala‐Campos et al ., 2022), 40,3,16 but their application to the production of butanol has received little attention. A previous study reported butanol production directly from cellulose using a fermentative microbial community bioaugmented with Clostridium saccharobutylicum .…”

Section: Introductionmentioning

confidence: 99%

“…8 Microbial communities are more competitive in transforming complex substrates such as lignocelluloses into bioproducts because they do not require exhaustive processing of the substrate; the costs of investment and operation are therefore much lower than for pure cultures. 15 Microbial communities have been very successful in producing biofuels such as methane, hydrogen, and ethanol directly from lignocellulose (Ayala-Campos et al, 2022), 40,3,16 but their application to the production of butanol has received little attention. A previous study reported butanol production directly from cellulose using a fermentative microbial community bioaugmented with Clostridium saccharobutylicum.…”

mentioning

confidence: 99%

Butanol and caproate production by consolidated bioprocessing after adaptive evolution of a fermentative microbial community

González-Tenorio

Dudek

Valdez‐Vazquez

2023

Biofuels Bioprod Bioref

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Butanol production from cellulose by consolidated bioprocessing may accelerate the commercialization of lignocellulosic biofuels. This study applied adaptive evolution to a natural microbial community consisting of different taxa of fermenters and plant‐degrading bacteria to increase its production of butanol. To this end, the parental microbial community was subjected to the stepwise enrichment of butanol at four initial concentrations in repeated‐batch fermentations using pretreated corn stover as the sole substrate. After the adaptive evolution, the microbial community exposed to the highest concentration of butanol produced 13.8 g L‐1 butanol, which was nine times higher than the parental microbial community. The adapted microbial community also produced the medium‐chain fatty acid caproate (7.5 g L‐1) from the condensation of alcohols (ethanol/butanol) with short‐chain fatty acids at the end of the fermentation. A network analysis revealed that the largest subcommunities were represented by Clostridium sensu stricto 7 and Caproiciproducens, which are associated with the production of alcohols (ethanol/butanol) and medium‐chain fatty acids, respectively. In the smaller subcommunities, Pseudomonas, Sutterella, and Pandoraea became more important after adaptive evolution, suggesting the production of extracellular polymeric substances and cross‐stress protection as mechanisms of protection and adaptation against butanol. This study demonstrated that butanol production by the consolidated bioprocessing of cellulose required solventogenic bacteria, diverse taxa of plant‐degrading bacteria that established unique interactions with the fermenters and, more important, members with mechanisms of protection and tolerance to solvents.

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Plant-associated microbial communities converge in fermentative hydrogen production and form a core microbiome

Cited by 9 publications

References 55 publications

Biohydrogen production through dark fermentation from waste biomass: Current status and future perspectives on biorefinery development

Biohydrogen production through dark fermentation from waste biomass: Current status and future perspectives on biorefinery development

Microbial activity of lactic acid bacteria and hydrogen producers mediated by pH and total solids during the consolidated bioprocessing of agave bagasse

Butanol and caproate production by consolidated bioprocessing after adaptive evolution of a fermentative microbial community

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