Planar and tubular patterning of micro and nano-topographies on poly(vinyl alcohol) hydrogel for improved endothelial cell responses

Cutiongco, Marie F.A.; Goh, S. H.; Aid‐Launais, Rachida; Visage, Catherine Le; Low, Hong Yee; Yim, Evelyn K.F.

doi:10.1016/j.biomaterials.2016.01.036

Cited by 89 publications

(89 citation statements)

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“…The increase of diameter and height of pillars with diameter to height 1:1 ratio also demonstrated an increasing trend of monocyte adhesion. Such influence of pillar topographies with various diameters on endothelial cell adhesion was shown previously [54] . However, to investigate these processes further, pillar topographies with various spatial ratios and lens topographies with various diameter and heights must be examined.…”

Section: Discussionsupporting

confidence: 74%

“…Although the reasons for lower cell adhesion are unclear, it is speculated that the characteristics of the pillars, wells and lens topographies could influence the cell sensing machinery. Micro- and nano-topography influence on cell-matrix interaction such as focal adhesion (FA) formation was demonstrated by various studies including the work of our group [48,54,55] . The cell sensing machinery is potentially tuned to the characteristic lengths of FA of vascular endothelial cells as previously described [56] .…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of the Topographical Influence on the Cellular Behavior of Human Umbilical Vein Endothelial Cells

et al. 2018

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Adhesion and proliferation of vascular endothelial cells are important parameters in the endothelialization of biomedical devices for vascular applications. Endothelialization is a complex process affected by endothelial cells and their interaction with the extracellular microenvironment. Although numerous approaches are taken to study the influence of the external environment, a systematic investigation of the impact of an engineered microenvironment on endothelial cell processes is needed. This study aims to investigate the influence of topography, initial cell seeding density, and collagen coating on human umbilical vein endothelial cells (HUVECs). Utilizing the MultiARChitecture (MARC) chamber, the effects of various topographies on HUVECs are identified, and those with more prominent effects were further evaluated individually using the MARC plate. Endothelial cell marker expression and monocyte adhesion assay are examined on the HUVEC monolayer. HUVECs on 1.8 μm convex and concave microlens topographies demonstrate the lowest cell adhesion and proliferation, regardless of initial cell seeding density and collagen I coating, and the HUVEC monolayer on the microlens shows the lowest monocyte adhesion. This property of lens topographies would potentially be a useful parameter in designing vascular biomedical devices. The MARC chamber and MARC plate show a great potential for faster and easy pattern identification for various cellular processes.

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Section: Discussionsupporting

confidence: 74%

Section: Discussionmentioning

confidence: 99%

Evaluation of the Topographical Influence on the Cellular Behavior of Human Umbilical Vein Endothelial Cells

et al. 2018

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“…Suturable poly(vinyl alcohol) hydrogel (PVA) tubes with 2μm luminal gratings improved endothelialization and reduced thrombotic occlusion as compared to non-patterned grafts. [148] Electrospinning is a powerful tool commonly used to generate fibrous scaffolds mimicking the architecture and topography of native extracellular matrix, [149] and may be supplemented with further spatial patterning. Electrospun polyurethane (PU) scaffolds with longitudinally aligned 3.6 μm ridges of 0.9 μm height patterned on the luminal surface had a physiologically relevant longitudinal elastic modulus of 0.43 MPa.…”

Section: Applications Of Vascular Mechanobiologymentioning

confidence: 99%

Endothelial Cell Mechanotransduction in the Dynamic Vascular Environment

2018

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The vascular endothelial cells (ECs) that line the inner layer of blood vessels are responsible for maintaining vascular homeostasis under physiological conditions. In the presence of disease or injury, ECs can become dysfunctional and contribute to a progressive decline in vascular health. ECs are constantly exposed to a variety of dynamic mechanical stimuli, including hemodynamic shear stress, pulsatile stretch, and passive signaling cues derived from the extracellular matrix. This review describes the molecular mechanisms by which ECs perceive and interpret these mechanical signals. The translational applications of mechanosensing are then discussed in the context of endothelial-to-mesenchymal transition and engineering of vascular grafts.

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“…However, nonspecific adhesion of non-EPC remains major concern as this can lead to problems such as restenosis, inflammation or hyperplasia 58 . Thus, physical and biochemical surface modifications have been seen to be necessary to improve the cell attachment on these synthetic polymers 53, 59, 60 . From a clinical point of view, the growth of ECs needs a fully defined microenvironment which could be provided by chemically defined synthetic polymers such as 8g7.…”

Section: Discussionmentioning

confidence: 99%

Poly(ethylmethacrylate-co-diethylaminoethyl acrylate) coating improves endothelial re-population, bio-mechanical and anti-thrombogenic properties of decellularized carotid arteries for blood vessel replacement

López‐Ruiz

Venkateswaran

Perán

et al. 2017

Sci Rep

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Decellularized vascular scaffolds are promising materials for vessel replacements. However, despite the natural origin of decellularized vessels, issues such as biomechanical incompatibility, immunogenicity risks and the hazards of thrombus formation, still need to be addressed. In this study, we coated decellularized vessels obtained from porcine carotid arteries with poly (ethylmethacrylate-co-diethylaminoethylacrylate) (8g7) with the purpose of improving endothelial coverage and minimizing platelet attachment while enhancing the mechanical properties of the decellularized vascular scaffolds. The polymer facilitated binding of endothelial cells (ECs) with high affinity and also induced endothelial cell capillary tube formation. In addition, platelets showed reduced adhesion on the polymer under flow conditions. Moreover, the coating of the decellularized arteries improved biomechanical properties by increasing its tensile strength and load. In addition, after 5 days in culture, ECs seeded on the luminal surface of 8g7-coated decellularized arteries showed good regeneration of the endothelium. Overall, this study shows that polymer coating of decellularized vessels provides a new strategy to improve re-endothelialization of vascular grafts, maintaining or enhancing mechanical properties while reducing the risk of thrombogenesis. These results could have potential applications in improving tissue-engineered vascular grafts for cardiovascular therapies with small caliber vessels.

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Planar and tubular patterning of micro and nano-topographies on poly(vinyl alcohol) hydrogel for improved endothelial cell responses

Cited by 89 publications

References 50 publications

Evaluation of the Topographical Influence on the Cellular Behavior of Human Umbilical Vein Endothelial Cells

Evaluation of the Topographical Influence on the Cellular Behavior of Human Umbilical Vein Endothelial Cells

Endothelial Cell Mechanotransduction in the Dynamic Vascular Environment

Poly(ethylmethacrylate-co-diethylaminoethyl acrylate) coating improves endothelial re-population, bio-mechanical and anti-thrombogenic properties of decellularized carotid arteries for blood vessel replacement

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