Planar and 3D interdigitated electrodes for biosensing applications: The impact of a dielectric barrier on the sensor properties

Bäcker, Matthias; Krämer, Friederike; Huck, Christina; Poghossian, Arshak; Bratov, Andrey; Abramova, Natalia; Schöning, Michael J.

doi:10.1002/pssa.201330416

Cited by 10 publications

(4 citation statements)

References 41 publications

(56 reference statements)

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“…On the other hand, it becomes obvious that the adsorption of positively charged PEI on the negatively charged BST sensor surface noticeably affects the real part of the impedance decreasing its value, e.g., by 17% at 1 Hz. Similar effect has been observed by PEI adsorption on the 3D‐IDE sensor without a protective layer and has been explained by the model based on the superimposition of the bulk and surface conductance .…”

Section: Resultssupporting

confidence: 82%

Multi‐parameter sensing using high‐k oxide of barium strontium titanate

Huck

Poghossian

Bäcker

et al. 2015

Physica Status Solidi (a)

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High‐k perovskite oxide of barium strontium titanate (BST) represents a very attractive multi‐functional transducer material for the development of (bio‐)chemical sensors. In this work, a Si‐based sensor chip containing Pt interdigitated electrodes covered with a thin BST layer (485 nm) has been developed for multi‐parameter chemical sensing. The chip has been applied for the contactless measurement of the electrolyte conductivity, the detection of adsorbed charged macromolecules (positively charged polyelectrolytes of polyethylenimine) and the concentration of hydrogen peroxide (H2O2) vapor. The experimental results of functional testing of individual sensors are presented. The mechanism of the BST sensitivity to charged polyelectrolytes and H2O2 vapor has been proposed and discussed.

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Section: Resultssupporting

confidence: 82%

Multi‐parameter sensing using high‐k oxide of barium strontium titanate

Huck

Poghossian

Bäcker

et al. 2015

Physica Status Solidi (a)

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“…Potential sources of error are the electrode height and the electrolyte complexity. Bäcker et al [ 51 ] compared planar and 3D IME for sensing and expanded on this basic model by considering a dielectric barrier between the electrode fingers. This approach offers potential improvement of the dielectric field if the geometry can support controlled immobilization of aptamer for promoting cell capture.…”

Section: Resultsmentioning

confidence: 99%

Planar Interdigitated Aptasensor for Flow-Through Detection of Listeria spp. in Hydroponic Lettuce Growth Media

Sidhu

Cavallaro

Pola

et al. 2020

Sensors

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Irrigation water is a primary source of fresh produce contamination by bacteria during the preharvest, particularly in hydroponic systems where the control of pests and pathogens is a major challenge. In this work, we demonstrate the development of a Listeria biosensor using platinum interdigitated microelectrodes (Pt-IME). The sensor is incorporated into a particle/sediment trap for the real-time analysis of irrigation water in a hydroponic lettuce system. We demonstrate the application of this system using a smartphone-based potentiostat for rapid on-site analysis of water quality. A detailed characterization of the electrochemical behavior was conducted in the presence/absence of DNA and Listeria spp., which was followed by calibration in various solutions with and without flow. In flow conditions (100 mL samples), the aptasensor had a sensitivity of 3.37 ± 0.21 kΩ log-CFU−1 mL, and the LOD was 48 ± 12 CFU mL−1 with a linear range of 102 to 104 CFU mL−1. In stagnant solution with no flow, the aptasensor performance was significantly improved in buffer, vegetable broth, and hydroponic media. Sensor hysteresis ranged from 2 to 16% after rinsing in a strong basic solution (direct reuse) and was insignificant after removing the aptamer via washing in Piranha solution (reuse after adsorption with fresh aptamer). This is the first demonstration of an aptasensor used to monitor microbial water quality for hydroponic lettuce in real time using a smartphone-based acquisition system for volumes that conform with the regulatory standards. The aptasensor demonstrated a recovery of 90% and may be reused a limited number of times with minor washing steps.

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“…This method requires redox couples to observe the changes, which makes it unsuitable for point-of-care measurements [ 9 ]. The non-faradaic (capacitive) method involves the passivation of the electrode, which eliminates the need for a redox couple to generate a signal, and measures the change in capacitance [ 10 , 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Label-Free, Impedance-Based Biosensor for Kidney Disease Biomarker Uromodulin

Vora,

Kordas,

Seidl

2023

Sensors

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We demonstrate the development of a label-free, impedance-based biosensor by using a passivation layer of 50-nm tantalum pentoxide (Ta2O5) on interdigitated electrodes (IDE). This layer was fabricated by atomic layer deposition (ALD) and has a high dielectric constant (high-κ), which improves the capacitive property of the IDE. We validate the biosensor’s performance by measuring uromodulin, a urine biomarker for kidney tubular damage, from artificial urine samples. The passivation layer is functionalized with uromodulin antibodies for selective binding. The passivated IDE enables the non-faradaic impedance measurement of uromodulin concentrations with a measurement range from 0.5 ng/mL to 8 ng/mL and with a relative change in impedance of 15 % per ng/mL at a frequency of 150 Hz (log scale). This work presents a concept for point-of-care biosensing applications for disease biomarkers.

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Planar and 3D interdigitated electrodes for biosensing applications: The impact of a dielectric barrier on the sensor properties

Cited by 10 publications

References 41 publications

Multi‐parameter sensing using high‐k oxide of barium strontium titanate

Multi‐parameter sensing using high‐k oxide of barium strontium titanate

Planar Interdigitated Aptasensor for Flow-Through Detection of Listeria spp. in Hydroponic Lettuce Growth Media

Label-Free, Impedance-Based Biosensor for Kidney Disease Biomarker Uromodulin

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