Placenta-Enriched LincRNAs MIR503HG and LINC00629 Decrease Migration and Invasion Potential of JEG-3 Cell Line

Muys, Bruna Rodrigues; Lorenzi, Julio C. C.; Zanette, Dalila Lucíola; Bueno, Rafaela de Barros Lima e; Araújo, Luíza Ferreira de; Dinarte-Santos, A. R.; Alves, Cleidson P.; Ramão, Anelisa; Molfetta, Greice Andreotti de; Vidal, Daniel Onofre; Silva, Wilson A.

doi:10.1371/journal.pone.0151560

Cited by 38 publications

(30 citation statements)

References 29 publications

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“…Originally, MIR503HG was identified to be involved in human endothelial cells and angiogenic processes . Soon afterwards, Muys et al found MIR503HG expression was increased in placenta, other reproductive tissues and 50% cancer cell lines. Then, Zhang et al found MIR503HG as an up‐regulated lncRNA in colorectal cancer tissues through analysing RNA‐seq datasets.…”

Section: Discussionmentioning

confidence: 99%

LncRNA MIR503HG inhibits cell migration and invasion via miR‐103/OLFM4 axis in triple negative breast cancer

Dong

Shi

et al. 2019

J Cellular Molecular Medi

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Long non‐coding RNA MIR503 host gene (MIR503HG) is located on chromosome Xq26.3, and has been found to be deregulated in many types of human malignancy and function as tumour suppressor or promoter based on cancer types. The role of MIR503HG in breast cancer was still unknown. In our study, we found MIR503HG expression was significantly decreased in triple‐negative breast cancer tissues and cell lines. Furthermore, we observed low MIR503HG expression was correlated with late clinical stage, lymph node metastasis and distant metastasis. In the survival analysis, we observed that triple‐negative breast cancer patients with low MIR503HG expression had a statistically significant worse prognosis compared with those with high MIR503HG expression, and low MIR503HG expression was a poor independent prognostic factor for overall survival in triple‐negative breast cancer patients. The study in vitro suggested MIR503HG inhibits cell migration and invasion via miR‐103/OLFM4 axis in triple negative breast cancer. In conclusion, MIR503HG functions as a tumour suppressive long non‐coding RNA in triple negative breast cancer.

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Section: Discussionmentioning

confidence: 99%

LncRNA MIR503HG inhibits cell migration and invasion via miR‐103/OLFM4 axis in triple negative breast cancer

Dong

Shi

et al. 2019

J Cellular Molecular Medi

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“…Expression of MIR503HG is similar to that of PLAC1 with expression found in placenta. Unlike PLAC1 , MIRG503HG expression is also found in human umbilical vein epithelial cells (HUVECS) [ 96 ]. By contrast, LINC00629 is expressed not only in the placenta but also in other tissues such as the ovary, cervix, testis and heart [ 96 ].…”

Section: Lncrnas Are Important For Trophoblast Cell Functionmentioning

confidence: 99%

“…Unlike PLAC1 , MIRG503HG expression is also found in human umbilical vein epithelial cells (HUVECS) [ 96 ]. By contrast, LINC00629 is expressed not only in the placenta but also in other tissues such as the ovary, cervix, testis and heart [ 96 ]. MIR503HG is localised to the nucleus of JEG-3 cells, while LINC00629 is found evenly throughout the cell.…”

Section: Lncrnas Are Important For Trophoblast Cell Functionmentioning

confidence: 99%

“…MIR503HG is localised to the nucleus of JEG-3 cells, while LINC00629 is found evenly throughout the cell. Overexpression of either of these lincRNAs in JEG-3 cells has been shown to reduce both migration (30% lower) and invasion (40% lower) [ 96 ]. By contrast, it has been reported that silencing of endogenous MIR503HG in HUVEC cells leads to a reduction in cell migration [ 97 ].…”

Section: Lncrnas Are Important For Trophoblast Cell Functionmentioning

confidence: 99%

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Mechanistic Insight into Long Noncoding RNAs and the Placenta

McAninch

Roberts

Bianco‐Miotto

2017

IJMS

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Long non-coding RNAs (lncRNAs) are classified as RNAs greater than 200 nucleotides in length that do not produce a protein product. lncRNAs are expressed with cellular and temporal specificity and have been shown to play a role in many cellular events, including the regulation of gene expression, post-transcriptional modifications and epigenetic modifications. Since lncRNAs were first discovered, there has been increasing evidence that they play important roles in the development and function of most organs, including the placenta. The placenta is an essential transient organ that facilitates communication and nutrient exchange between the mother and foetus. The placenta is of foetal origin and begins to form shortly after the embryo implants into the uterine wall. The placenta relies heavily on the successful differentiation and function of trophoblast cells, including invasion as well as the formation of the maternal/foetal interface. Here, we review the current literature surrounding the involvement of lncRNAs in the development and function of trophoblasts and the human placenta.

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“…11995065). The other cells lines used in the tumor cell line panel to compare with normal tissues were previously described 36 . All mediums were supplemented with 10% FBS (GE Healthcare, Catalog No.…”

Section: Cell Culture Maintenancementioning

confidence: 99%