Place of diagnostic tools in the identification of Anaerobiospirillum succiniciproducens bacteraemia

Decroix, Véronique; Pluquet, E.; Choquet, Morgane; Ammenouche, N.; Castelain, Sandrine; Guihéneuf, Raphaël

doi:10.1016/j.anaerobe.2016.02.005

Cited by 5 publications

(5 citation statements)

References 12 publications

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“…The Rapid ID 32A system (bioMérieux, Durham, NC) has been used to successfully identify A. succiniciproducens in a few cases (4). In a recent publication, another MALDI-TOF MS system, MALDI Biotyper (Bruker Daltonics, Bremen, Germany), failed to identify A. succiniciproducens (5). That Vitek MS successfully identified the organism in our case may reflect differences in the databases or other features of commercially available systems.…”

Section: Discussionmentioning

confidence: 76%

The Brief Case: Anaerobiospirillum succiniciproducens Bacteremia and Pyomyositis

et al. 2017

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A 39-year-old man presented to the Bellevue Hospital emergency department in November with 1 week of fevers and cough. On the previous day, he had been assaulted, developing right buttock and thigh pain.His medical history was notable for alcohol use disorder treated with the opioid antagonist naltrexone (depot formulation), injected intramuscularly into the gluteal region monthly. He received left and right gluteal injections 1 and 6 weeks prior to presentation, respectively. He was homeless and denied travel or animal contact. He drank up to two dozen beer cans daily and smoked cigarettes but denied drug use.On admission, he appeared ill and disheveled. His temperature was 38.3°C, his pulse was 124 beats per minute, and oxygen saturation was 94%; blood pressure and respiratory rate were normal. He had ecchymoses on the right buttock and thigh. His white blood cell count was 2.3 ϫ 10 3 /l (differential of 87% polymorphonuclear leukocytes and 8% band leukocytes), hemoglobin was 8.4 g/dl, and platelets were 45 ϫ 10 3 /l. Transaminases, alkaline phosphatase, and creatine kinase were elevated; albumin was low. Radiographs of the chest, pelvis, and femur were unremarkable. Direct antigen tests for influenza viruses were negative.After blood cultures were obtained, ceftriaxone and azithromycin were administered for treatment of pneumonia; however, fevers persisted, and hypotension and altered mentation developed. On day two, antibiotics were empirically changed to vancomycin and cefepime due to clinical deterioration. An anaerobic blood culture bottle (Bactec 9240 System; Becton, Dickinson Inc., Franklin Lakes, NJ) became positive after 3 days of incubation, and Gram staining showed spiral-shaped Gram-negative organisms (Fig. 1). Broth was subcultured onto Trypticase soy agar with 5% sheep blood (blood agar plate [BAP]) and MacConkey, chocolate, and Campylobacter blood agar plates (BBL prepared plated media; Becton, Dickinson Inc., Franklin Lakes, NJ). BAP incubated anaerobically yielded small, translucent, spreading, nonhemolytic colonies that were oxidase and catalase negative (Fig. 2). The organism was identified as Anaerobiospirillum succiniciproducens by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) using the FDA-approved Vitek MS version MS-CE CLI 2.0.0 on intact cells without extraction (bioMérieux Inc., Durham, NC). Although the species did not have a claimed or validated identification in the instrument database, the confidence value was 99.9, and its identification was subsequently confirmed by 16S rRNA

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Section: Discussionmentioning

confidence: 76%

The Brief Case: Anaerobiospirillum succiniciproducens Bacteremia and Pyomyositis

et al. 2017

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“…bacteraemia, abdominal infection) and mainly affects immunocompromised patients [3], [5]. Here, we report the first case of an A. succiniciproducens prosthetic joint infection.…”

Section: Discussionmentioning

confidence: 93%

First description of an Anaerobiospirillum succiniciproducens prosthetic joint infection

Schaumburg

Dieckmann

Schmidt-Bräkling

et al. 2017

New Microbes and New Infections

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“…Diarrheal infections occur in healthy patients [4, 6] and one case of bacteremia was reported in a healthy man [2]. Mortality rate of infections due to Anaerobiospirillum approximate 30% [5].…”

Section: Discussionmentioning

confidence: 99%

“…A. succiniciproducens is thought to be part of normal gastrointestinal flora of some dogs and cats [1], but not humans [2]. Infections due to Anaerobiospirillum are rare but instances of bacteremia, pyomyositis, gastrointestinal infections, and one case of knee prosthetic joint infection (PJI) has been described [2–5]. These infections typically in immunocompromised hosts or patients with comorbid medical conditions.…”

Section: Introductionmentioning

confidence: 99%

Case report: Anaerobiospirillum prosthetic joint infection in a heart transplant recipient

Madden

Poulter

Crawford

et al. 2019

BMC Musculoskelet Disord

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Background We report a case of prosthetic hip joint infection in a heart transplant recipient due to Anaerobiospirillum succiniciproducens, a genus of spiral-shaped curved anaerobic gram-negative rod which colonizes the gastrointestinal tract of cats and dogs. Invasive infections in humans are rare and typically occur in immunocompromised hosts. Case presentation A 65-year-old male dog breeder with a history of rheumatoid arthritis, bilateral hip arthroplasties, and non-ischemic cardiomyopathy with a heart transplant 10 years ago presented with a three month history of progressive left hip pain and frank purulence on hip aspiration. He underwent irrigation and debridement of the left hip and one-stage revision with hardware exchange. Although gram stain and culture from synovial fluid and intraoperative cultures were initially negative, anaerobic cultures from tissue specimens later grew a spiral-shaped gram-negative rod, identified as Anaerobiospirillum spp. by 16S rRNA gene sequencing. The patient was treated with ceftriaxone 2 g daily for 6 weeks with a good response to treatment. A similar organism was unable to be isolated from culture of 2 of the patient’s dogs, however, they were thought to be the most likely source of his infection. Conclusion Anaerobiospirillum spp . should be considered in immunocompromised patients with exposure to dogs or cats who present with bacteremia, gastrointestinal infection, pyomyositis, or prosthetic joint infections, especially in cases of culture-negative or with anaerobic culture growth.

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Place of diagnostic tools in the identification of Anaerobiospirillum succiniciproducens bacteraemia

Cited by 5 publications

References 12 publications

The Brief Case: Anaerobiospirillum succiniciproducens Bacteremia and Pyomyositis

The Brief Case: Anaerobiospirillum succiniciproducens Bacteremia and Pyomyositis

First description of an Anaerobiospirillum succiniciproducens prosthetic joint infection

Case report: Anaerobiospirillum prosthetic joint infection in a heart transplant recipient

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