PKHhigh cells within clonal human nephrospheres provide a purified adult renal stem cell population

Bombelli, S; Zipeto, M; Torsello, B; Bovo, Giorgio; Stefano, Vitalba Di; Bugarin, Cristina; Zordan, Paola; Viganó, Paolo; Cattoretti, Giorgio; Strada, G; Bianchi, Cristina; Perego, R

doi:10.1016/j.scr.2013.08.004

Cited by 29 publications

(48 citation statements)

References 44 publications

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“…CD24/CD133 double-positive cells were localized in the tubular epithelium, and demonstrated clonogenic multipotency and self-renewal ability [28] . Bombelli et al [30] recently proposed existence of CD133 + /CD24…”

Section: Mrpc)mentioning

confidence: 99%

Adult stem-like cells in kidney

Hishikawa

Takase

Yoshikawa

et al. 2015

WJSC

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Human pluripotent cells are promising for treatment for kidney diseases, but the protocols for derivation of kidney cell types are still controversial. Kidney tissue regeneration is well confirmed in several lower vertebrates such as fish, and the repair of nephrons after tubular damages is commonly observed after renal injury. Even in adult mammal kidney, renal progenitor cell or system is reportedly presents suggesting that adult stem-like cells in kidney can be practical clinical targets for kidney diseases. However, it is still unclear if kidney stem cells or stem-like cells exist or not. In general, stemness is defined by several factors such as self-renewal capacity, multi-lineage potency and characteristic gene expression profiles. The definite use of stemness may be obstacle to understand kidney regeneration, and here we describe the recent broad findings of kidney regeneration and the cells that contribute regeneration.

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Section: Mrpc)mentioning

confidence: 99%

Adult stem-like cells in kidney

Hishikawa

Takase

Yoshikawa

et al. 2015

WJSC

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“…Working in the opposite direction, citing the idea that markers are not 100% accurate, Bombelli et al isolated a potential stem cell population based off of self-renewal capabilities [65]. Using sphere-forming methods of culture, they found a population of human renal cells that was able to form nephrospheres.…”

Section: Renal Stem Cells From Tubular Originsmentioning

confidence: 99%

Comparing adult renal stem cell identification, characterization and applications

Huling

Yoo

2017

J Biomed Sci

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Despite growing interest and effort, a consensus has yet to be reached in regards to the identification of adult renal stem cells. Organ complexity and low turnover of renal cells has made stem cell identification difficult and lead to the investigation of multiple possible populations. In this review, we summarize the work that has been done toward finding and characterizing an adult renal stem cell population. In addition to giving a general overview of what has been done, we aim to highlight the variation in methods and outcomes. The methods used to locate potential stem cell populations can vary widely, but even within the relatively standard practice of BrdU labeling of slowly dividing cells, there are significant differences in protocols and results. Additional diversity exists in cell marker profiles and apparent differentiation potential seen in potential stem cell sources. Cataloging the variety of methods and outcomes seen so far may help to streamline future investigation and stear the field toward consensus. But even without firmly defined populations, the application of renal stem cells holds tantalizing potential. Populations of highly proliferative, multipotent cells of renal origin show the ability to engraft in injured kidneys, mitigate functional loss and occasionally show the ability to generate nephrons de novo. The progress toward regenerative medicine applications is also summarized.

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“…79,80 Consistent with this finding, a CD133 + CD24 -population was identi fied within spheres cultured from single human adult kidney cells. 81 This population is capable of selfrenewal and differentiation into multiple renal cell types when cultured in differentiation conditions.…”

Section: Challengesmentioning

confidence: 99%

Development and application of human adult stem or progenitor cell organoids

et al. 2015

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Adult stem or progenitor cell organoids are 3D adult-organ-derived epithelial structures that contain self-renewing and organ-specific stem or progenitor cells as well as differentiated cells. This organoid culture system was first established in murine intestine and subsequently developed for several other organs and translated to humans. Organoid cultures have proved valuable for basic research and for the study of healthy tissue homeostasis and the biology of disease. In addition, data from proof-of-principle experiments support promising clinical applications of adult stem or progenitor cell organoids. Although renal organoids have many potential applications, an adult stem or progenitor cell organoid culture system has not yet been developed for the kidney. The development of such a system is likely to be challenging because of the intricate renal architecture. Differentiated 3D cultures and stem or progenitor cell 3D sphere cultures are, however, available for the kidney. These cultures indicate the feasibility of renal organoid culture and provide a solid basis for its development. In this Review, we discuss the state-of-the-art of human adult stem or progenitor cell organoid culture and the potential of renal organoids as tools in basic and clinical research.

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PKHhigh cells within clonal human nephrospheres provide a purified adult renal stem cell population

Cited by 29 publications

References 44 publications

Adult stem-like cells in kidney

Adult stem-like cells in kidney

Comparing adult renal stem cell identification, characterization and applications

Development and application of human adult stem or progenitor cell organoids

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