Pinostilbene Hydrate Suppresses Human Oral Cancer Cell Metastasis by Downregulation of Matrix Metalloproteinase-2 Through the Mitogen-Activated Protein Kinase Signaling Pathway

Abstract

Background/Aims: Cancer is the most common cause of death worldwide with approximately one third of people being diagnosed with cancer in their lifetime. Pinostilbene hydrate (PSH) A methylated derivative of resveratrol Has been reported to possess antioxidative Cardioprotective and anticancer properties. However the antimetastatic effect of pinostilbene in oral squamous cell carcinoma (OSCC) remains unknown. Methods: In this study We investigated the effect of PSH on antimetastatic activity and the relevant signaling pathways underlying mechanisms of SCC-9 SAS and HSC-3 oral cancer cell lines by MTT assay Wound healing Transwell assay Zymography and western blot analysis. Results: Our findings indicated that PSH inhibits migration and invasion ability by reducing the protein activity and expression of matrix metalloproteinases-2 (MMP-2) in all three cell lines. Moreover • The phosphorylation of extracellular signal-regulated kinase (ERK) and p38 mitogen-activated protein kinases (p38) had significant inhibitory effects in the presence of PSH in the SCC9 and SAS cell lines. A combination of ERK1/2 and p38 inhibitors with PSH also reduced the migration and activity of MMP-2 in the SCC9 and SAS cell lines. Conclusion: This study demonstrated that PSH suppresses MMP-2 enzymatic activity by downregulating the p38/ERK1/2 pathway and that it might be a promising agent for preventing OSCC cell metastasis.

Geraniin inhibits oral cancer cell migration by suppressing matrix metalloproteinase‐2 activation through the FAK/Src and ERK pathways

“…In addition, the activation ratio of MMP‐2 significantly increased in patients with lymph node metastasis compared with patients without lymph node metastasis . The inhibition of MMP‐2 protein expression can effectively reduce the migratory ability of oral cancer cells …”

“…51 The inhibition of MMP-2 protein expression can effectively reduce the migratory ability of oral cancer cells. 52,53 To elucidate the mechanism of geraniin's suppression of the invasion and migration of oral cancer cells by the downregulation of MMP-2, the signaling pathways that are frequently involved in the tumorigenesis in human cancers were examined. The present study revealed that FAK, Src, and MAPK ERK1/2 were involved in the process ( Figure 5).…”

Dual Targeting of the p38 MAPK-HO-1 Axis and cIAP1/XIAP by Demethoxycurcumin Triggers Caspase-Mediated Apoptotic Cell Death in Oral Squamous Cell Carcinoma Cells

“…Kasten, East Tennessee State University, Quillen College of Medicine, Johnson City, TN. Culture conditions of both OSCC cell lines were maintained in Dulbecco's Modified Eagle Medium/Ham's F12 Nutrient Mixture (DMEM/F12; Life Technologies, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS) (Gibco, Grand Island, NY, USA) and other essential supplements as previously described [60]. The SG cells were cultured in DMEM medium supplemented with 10% FBS.…”

Sodium Danshensu Inhibits Oral Cancer Cell Migration and Invasion by Modulating p38 Signaling Pathway

“…Another oral cancer cell line, Ca9-22, was obtained from and authenticated by the Japanese Collection of Research Bioresources Cell Bank (JCRB, Shinjuku, Japan). Dulbecco's Modified Eagle Medium (DMEM; Life Technologies, Grand Island, NY) containing nutritional supplements (as described previously) was used to culture the cells (18). The cells were kept inside an incubator, under humidified condition (temp: 37 • C; CO 2 : 5%).…”

“…Trans-well assay was carried out to examine the migratory and invasive properties of FaDu and CA9-22 cells (Greiner Bio-One, North Carolina, USA). The cells were seeded onto transwell inserts (the upper well containing serum-free medium), incubated for 24 h, and allowed to migrate, as defined earlier (18,20). Next, the cells were examined and photographed under a light microscope for the analysis of cellular migration and invasion.…”