PilM/N/O/P Proteins Form an Inner Membrane Complex That Affects the Stability of the Pseudomonas aeruginosa Type IV Pilus Secretin

Ayers, M.; Sampaleanu, L.M.; Tammam, Stephanie; Koo, Jarley; Harvey, Hanjeong; Howell, P. Lynne; Burrows, Lori L.

doi:10.1016/j.jmb.2009.09.034

Cited by 116 publications

(152 citation statements)

References 81 publications

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“…The PilP lipoprotein is formed from a long, flexible N terminus, which contains the lipid attachment site, and a globular C-terminal domain (35). The C-terminal domain binds to PilQ (8) and the N terminus recognizes PilN as part of the PilMNO complex (13,36). It therefore forms a crucial link between the dodecameric secretin assembly and the PilMNO inner membrane platform.…”

Section: Discussionmentioning

confidence: 99%

Structure and assembly of an inner membrane platform for initiation of type IV pilus biogenesis

Karuppiah

Collins

Thistlethwaite

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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Significance Type IV pili are long, thin fibers, formed mainly of polymers of a single pilin protein, which are displayed on the surfaces of many bacteria, including several human pathogens. Here, we report three-dimensional reconstructions of the PilMNO inner membrane complex, alone and in complex with pilin protein, through a combination of X-ray crystallography and electron microscopy. PilMNO forms a dimeric T-shaped structure, binding two copies of the pilin protein at its extremities. The results provide a structural model for the way in which pilin is harvested from the inner membrane and made available to other components of the type IV pilus biogenesis machinery.

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Section: Discussionmentioning

confidence: 99%

Structure and assembly of an inner membrane platform for initiation of type IV pilus biogenesis

Karuppiah

Collins

Thistlethwaite

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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“…Western Blot Analysis-Whole cell lysate samples were separated on 15% SDS-polyacrylamide gels at 80 -120 V. Samples were transferred to nitrocellulose membranes at 225 mA for 1 h. The membranes were blocked in 5% (w/v) skim milk in 1ϫ PBS (pH 7.0) for 1 h, followed by incubation in rabbit antisera specific for the proteins of interest (34,37,38) for 18 h at 4°C. The anti-PilC antibody was generated using a peptide antigen corresponding to residues 81-102 of P. aeruginosa PilC (in the first cytoplasmic domain) to immunize rabbits (Cedarlane Laboratories, Burlington, Ontario, Canada).…”

Section: Methodsmentioning

confidence: 99%

“…PilMNOP was proposed to function as a "scaffold" to connect pilin subunits embedded in the inner membrane with the cytoplasmic ATPases (26). Although studies in Myxococcus, Thermus, and Pseudomonas suggested that alignment subcomplex proteins are important for pilus expression and twitching motility (14,(32)(33)(34), none examined the phenotypes of retraction-deficient double mutants (35).…”

mentioning

confidence: 99%

The Platform Protein Is Essential for Type IV Pilus Biogenesis

Takhar

Kemp

Kim

et al. 2013

Journal of Biological Chemistry

109

118

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Background:The platform protein PilC and/or the alignment subcomplex PilMNOP may coordinate type IV pilus (T4P) extension/retraction ATPases. Results: Loss of PilC in a retraction-deficient background abolished assembly. C-terminal mutations were permissive for assembly but not twitching motility. Conclusion: PilC is essential for pilus biogenesis and twitching motility. Significance: Platform proteins are conserved essential components of T4P and type II secretion machines.

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“…Studies have shown that disruption of the pilP gene, results in a loss of surface type IV pili and a deficiency in twitching motility (Ayers et al, 2009). We demonstrated partial restoration of twitching motility following the introduction of a wildtype pilP on a plasmid, implicating this mutation in the defective twitching motility of PSE306.…”

Section: Discussionmentioning

confidence: 60%

“…the type IV pilus (Ayers et al, 2009). Studies have shown that disruption of the pilP gene, results in a loss of surface type IV pili and a deficiency in twitching motility (Ayers et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

Divergence of a strain of Pseudomonas aeruginosa during an outbreak of ovine mastitis

Wright

Lorenzo

Trappetti

et al. 2015

Veterinary Microbiology

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Bacterial infections causing mastitis in sheep can result in severe economic losses for farmers. A large survey of milk samples from ewes with mastitis in Sardinia, Italy, indicated an increasing prevalence of Pseudomonas aeruginosa infections. It has been shown previously that during chronic, biofilm-associated infections P. aeruginosa populations diversify. We report the phenotypic and genomic characterisation of two clonal P. aeruginosa isolates (PSE305 and PSE306) from a mastitis infection outbreak, representing distinct colony morphology variants. In addition to pigment production, PSE305 and PSE306 differed in phenotypic characteristics including biofilm formation, utilisation of various carbon and nitrogen sources, twitching motility. We found higher levels of expression of genes associated with biofilm formation (pelB) and twitching motility (flgD) in PSE305, compared to the biofilm and twitching-defective PSE306. Comparative genomics analysis revealed single nucleotide polymorphisms (SNPs) and minor insertion/deletion variations between PSE305 and PSE306, including a SNP mutation in the pilP gene of PSE306. By introducing a wild-type pilP gene we were able to partially complement the defective twitching motility of PSE306. There were also three larger regions of difference between the two genomes, indicating genomic instability. Hence, we have demonstrated that P. aeruginosa population divergence can occur during an outbreak of mastitis, leading to significant variations in phenotype and genotype, and resembling the behaviour of P. aeruginosa during chronic biofilm-associated infections. ß

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PilM/N/O/P Proteins Form an Inner Membrane Complex That Affects the Stability of the Pseudomonas aeruginosa Type IV Pilus Secretin

Cited by 116 publications

References 81 publications

Structure and assembly of an inner membrane platform for initiation of type IV pilus biogenesis

Structure and assembly of an inner membrane platform for initiation of type IV pilus biogenesis

The Platform Protein Is Essential for Type IV Pilus Biogenesis

Divergence of a strain of Pseudomonas aeruginosa during an outbreak of ovine mastitis

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