Picosecond time-resolved laser spectrometer with expanded delay range

Johnson, Carey K.; Bostick, J. M.; Mounter, Sarah A.; Ratzlaff, K.; Schloemer, David E.

doi:10.1063/1.1139911

Cited by 10 publications

(4 citation statements)

References 6 publications

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“…The energy of the probe pulses was limited to less than 0.01 ftJ with neutral optical density filters. The laser system (Johnson et al, 1988) generates pump and probe pulses of about 50 ps duration with time delays between pump and probe variable from the picosecond to the second time scales. The pulse repetition rate of 30 Hz was selected so that the time between two sequential pump pulses was longer than the photocycle completion time.…”

Section: Methodsmentioning

confidence: 99%

Reorientations in the Bacteriorhodopsin Photocycle

Qin¹,

Harms²,

Wan³

et al. 1994

Biochemistry

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Reversible photoinduced reorientations of bacteriorhodopsin have been detected in suspensions of the purple membrane of Halobacterium salinarium. The anisotropy in bacteriorhodopsin during the nanosecond through millisecond stages of the photocycle was measured by time-resolved linear dichroism and transient absorption measurements. From these measurements the anisotropies of the K, L, M, and O intermediates were determined and related to the chromophore orientation with respect to the initially selected orientation. The anisotropies of the K and L states are 0.38 +/- 0.01 and 0.35 +/- 0.01, respectively. Further anisotropy decay after formation of the M intermediate in about 0.5 ms is evidence of orientational motion at this stage in the photocycle. A constant anisotropy with a value of 0.39 +/- 0.02 in the O intermediate demonstrates a recovery of the initial protein orientation with the formation of the O state. These results demonstrate that reorientations in BR are photoinduced and reversible. Similar measurements for L and M were carried out for purple membrane in polyacrylamide gels, where the anisotropies in the L and M states are 0.38 +/- 0.014 and 0.36 +/- 0.01, respectively. These results show that reorientations also occur in BR immobilized in gels. Anisotropy decay in the M state after formation of the M intermediate was not detected in the gels, in contrast to the M intermediate in suspensions. Orientational changes are observed for BR in purple membrane suspensions in the K state, during the K-->L step, in the M state possibly related to an M1-->M2 transition, and in the O state, where an almost complete return to the original orientation occurs.(ABSTRACT TRUNCATED AT 250 WORDS)

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Section: Methodsmentioning

confidence: 99%

Reorientations in the Bacteriorhodopsin Photocycle

Qin¹,

Harms²,

Wan³

et al. 1994

Biochemistry

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“…In instruments that use pulsed-detection, usually optical delay lines (mechanical delay stages) are used to achieve time-delays spanning fs-ns timescales, whilst phase-shifting methods can be used to achieve ps-ns time-delays, and pulsepicking and multiple-probe methods are typically used to extend the range of ultrafast instruments to 4ns timescales. 21,[24][25][26][27][28] In TAS/TRIR, the intensity of the visible/IR probe passing through the sample with and without photoexcitation is detected, from which the change in absorption (DA) caused by photoexcitation can be calculated. The transient signal generally has contribution from multiple components.…”

Section: Fundamentals Of Tas and Trirmentioning

confidence: 99%

Charge carrier dynamics and reaction intermediates in heterogeneous photocatalysis by time-resolved spectroscopies

Miao

Tang

2022

Chem. Soc. Rev.

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“…[4][5][6][7] Modern time-resolved spectrometers typically achieve their time resolution in two ways, electronic control of light sources or detectors (> ns) 8,9 and translational control of ultrafast laser beam path lengths (< ns). 10 Crossing this barrier is possible using a combination of translation stages and electronic timing control with ultrafast lasers, [11][12][13][14] and is important to be able to reliably compare data from experiments which demonstrate dynamic processes in both regions, rather than collecting data with multiple instruments.…”

Section: Introductionmentioning

confidence: 99%

Time-resolved multiple probe spectroscopy

Greetham¹,

Solé²,

Clark³

et al. 2012

Review of Scientific Instruments

119

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Time-resolved multiple probe spectroscopy combines optical, electronic, and data acquisition capabilities to enable measurement of picosecond to millisecond time-resolved spectra within a single experiment, using a single activation pulse. This technology enables a wide range of dynamic processes to be studied on a single laser and sample system. The technique includes a 1 kHz pump, 10 kHz probe flash photolysis-like mode of acquisition (pump-probe-probe-probe, etc.), increasing the amount of information from each experiment. We demonstrate the capability of the instrument by measuring the photolysis of tungsten hexacarbonyl (W(CO)(6)) monitored by IR absorption spectroscopy, following picosecond vibrational cooling of product formation through to slower bimolecular diffusion reactions on the microsecond time scale.

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Picosecond time-resolved laser spectrometer with expanded delay range

Cited by 10 publications

References 6 publications

Reorientations in the Bacteriorhodopsin Photocycle

Reorientations in the Bacteriorhodopsin Photocycle

Charge carrier dynamics and reaction intermediates in heterogeneous photocatalysis by time-resolved spectroscopies

Time-resolved multiple probe spectroscopy

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