PIBF (progesterone induced blocking factor) is overexpressed in highly proliferating cells and associated with the centrosome

Lachmann, Margit; Gelbmann, Dieter; Kálmán, Endre; Polgar, Beata; Buschle, Michael; Gabain, Alexander von; Szekeres-Barthó, Júlia; Nagy, Eszter

doi:10.1002/ijc.20326

Cited by 71 publications

(112 citation statements)

References 33 publications

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“…Recently, overexpression of this gene was associated with breast cancer and highly proliferating cells. The PIBF1 protein is associated with the centrosome (28,29). Notably, loss of progesterone receptor in meningioma patients is correlated with tumor grade and a poor prognosis (30,31).…”

Section: Discussionmentioning

confidence: 99%

Complex humoral immune response against a benign tumor: Frequent antibody response against specific antigens as diagnostic targets

Comtesse

Zippel

Walle

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

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There are numerous studies on the immune response against malignant human tumors. This study was aimed to address the complexity and specificity of humoral immune response against a benign human tumor. We assembled a panel of 62 meningiomaexpressed antigens that show reactivity with serum antibodies of meningioma patients, including 41 previously uncharacterized antigens by screening of a fetal brain expression library. We tested the panel for reactivity with 48 sera, including sera of patients with common-type, atypical, and anaplastic meningioma, respectively. Meningioma sera detected an average of 14.6 antigens per serum and normal sera an average of 7.8 antigens per serum (P ‫؍‬ 0.0001). We found a decline of seroreactivity with malignancy with a statistical significant difference between common-type and anaplastic meningioma (P < 0.05). We detected 17 antigens exclusively with patient sera, including 12 sera that were reactive against KIAA1344, 9 against natural killer tumor recognition (NKTR), and 7 against SRY (sex determining region Y)-box2 (SOX2). More than 80% of meningioma patients had antibodies against at least one of the antigens KIAA1344, SC65, SOX2, and C6orf153. Our results show a highly complex but specific humoral immune response against a benign tumor with a distinct serum reactivity pattern and a decline of complexity with malignancy. The frequent antibody response against specific antigens offers new diagnostic and therapeutic targets for meningioma. We developed a statistical learning method to differentiate sera of meningioma patients from sera of healthy donors. meningioma

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Section: Discussionmentioning

confidence: 99%

Complex humoral immune response against a benign tumor: Frequent antibody response against specific antigens as diagnostic targets

Comtesse

Zippel

Walle

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

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“…Sequencing analysis showed that the 52 clones represented 30 distinct antigens (Table 1). Of these 30 antigens, one is the CT antigen CAGE protein, and the other 13 antigens, which had previously been reported to be involved in tumorigenesis or suspected to be involved in tumour progression, including CDC37 (Stepanova et al, 2000), MIF (Li et al, 2004), galectin 4 (Huflejt and Leffler, 2004), galectin 8 (Bidon-Wagner and Le Pennec, 2004), PINCH (WangRodriguez et al, 2002), SPRY2 (Lee et al, 2004a), HSPCA (Becker et al, 2004;Huang et al, 2004b), transgelin 2 (Shields et al, 2002), HDAC2 , H factor (Junnikkala et al, 2000), AAT (Huang et al, 2004a), B factor (Perou et al, 1999), PIBF (Lachmann et al, 2004). Three other antigens (SR140 protein, SFRS2IP, RNPC2) may be involved in the regulation of alternative mRNA splicing, PSMA7 is related to hepatitis B and hepatitis C viral replication (Zhang et al, 2000;Kruger et al, 2001), and the remaining 12 antigens have no known association with cancer or hepatitis B or hepatitis C.…”

Section: Serex Defined Hcc-associated Antigensmentioning

confidence: 99%

Identification and analysis of tumour-associated antigens in hepatocellular carcinoma

Wang

et al. 2005

Br J Cancer

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To identify tumour and tumour-associated antigens in patients with hepatocellular carcinoma (HCC) one may find potential diagnostic markers and immunotherapeutic targets. In the current study, 30 distinct antigens reactive with serum IgG from HCC patients were identified by serological analysis of cDNA expression libraries (SEREX). The mRNA expression patterns of 14 of these 30 antigens were altered in cancer as further revealed by cDNA microarray, with upregulation for nine and downregulation for five antigens. One of the upregulated antigens was cancer-testis (CT) antigen (CAGE), which had been previously reported to be expressed exclusively in normal gametogenic tissues and aberrantly expressed in a variety of cancer cells. In our study, CAGE mRNA was expressed in 39.4% of HCC patients, 73.3% of patients with gastric cancer and 30.8% of patients with colorectal cancer. Antibodies against CAGE protein were detected in approximately 5.1% of the sera from HCC patients, 8.3% of that from gastric cancer patients and 7.3% of that from colorectal cancer patients. The relative high incidence of CAGE in cancer cells makes it a potential target for vaccine design. Another antigen of great interest is transgelin 2. The overexpression of transgelin 2 mRNA in a large per cent (69%) of HCC points to its potential as a diagnostic marker for HCC.

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“…There is an immunomodulatory protein known as the progesterone induced blocking factor (PIBF) that is overexpressed in highly proliferating cells [1]. The parent compound, which measures 90 kDa, resides in the nucleus at a centrosomal position [1].…”

Section: Introductionmentioning

confidence: 99%

“…The parent compound, which measures 90 kDa, resides in the nucleus at a centrosomal position [1]. This protein seems to be unique in that it shows no significant amino acid sequence homology with any known protein [2].…”

Section: Introductionmentioning

confidence: 99%

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Evidence that exposure to progesterone alone is a sufficient stimulus to cause a precipitous rise in the immunomodulatory protein the progesterone induced blocking factor (PIBF)

Cohen

Check

Dougherty

2015

J Assist Reprod Genet

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Purpose To determine if exposure to progesterone alone is sufficient to increase the production of the immunomodulatory protein known as the progesterone induced blocking factor (PIBF). Also to determine what method of progesterone delivery or form of P best stimulates PIBF secretion. Methods Serum samples from patients with infertility and paid volunteers were evaluated for both PIBF and progesterone at various times during the follicular phase and the luteal phase in both natural cycles and cycles involving embryo transfer after endogenous and exogenous progesterone exposure and after various synthetic progestins. PIBF was measured by a non-commercial research ELISA assay. Comparisons were made of serum PIBF before and after exposure to progesterone, 17-hydroxyprogesterone, and oral contraceptives. PIBF was also measured before and after transfer of embryos.Results Progesterone alone without exposure to the fetal allogeneic stimulus was able to produce a marked increase in s e r u m P I B F. N e i t h e r a s y n t h e t i c p r o g e s t i n (19-nortestosterone derivative) nor 17-hydroxyprogesterone caused an increase in PIBF. Some PIBF is generally detected even in the follicular phase. Conclusions A previous concept considered that an allogeneic stimulus, e.g., from the fetal semi-allograft, was necessary to induce de novo progesterone receptors in gamma delta T cells, which, in turn, when exposed to a high concentration of progesterone, would secrete high levels of PIBF. These data show that exposure to an allogeneic stimulus is not needed to cause a marked rise in PIBF, merely progesterone alone is sufficient.

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PIBF (progesterone induced blocking factor) is overexpressed in highly proliferating cells and associated with the centrosome

Cited by 71 publications

References 33 publications

Complex humoral immune response against a benign tumor: Frequent antibody response against specific antigens as diagnostic targets

Complex humoral immune response against a benign tumor: Frequent antibody response against specific antigens as diagnostic targets

Identification and analysis of tumour-associated antigens in hepatocellular carcinoma

Evidence that exposure to progesterone alone is a sufficient stimulus to cause a precipitous rise in the immunomodulatory protein the progesterone induced blocking factor (PIBF)

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