PI3K inhibition highlights new molecular interactions involved in the skeletogenesis of Paracentrotus lividus embryos

Chiaramonte, Marco; Russo, Roberta; Costa, Caterina; Bonaventura, Rosa; Zito, Francesca

doi:10.1016/j.bbamcr.2019.118558

Cited by 10 publications

(18 citation statements)

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“…In parallel with the cytoskeleton reorganisation and the initiation of the endocytic and phagocytic processes that lead to the digestion of pathogen organisms, bacterial LPS can also initiate protective strategies in which the histone proteins can act as antimicrobial proteins. The establishment of a stress process also assists this defense mechanism [58][59][60][61][62][63][64]. Indeed, our study has shown the activation of stress processes in the Mediterranean P. lividus sea urchin, at 1HPLT, through the presence of a protein-protein cluster basically constituted of HSP proteins (Fig 3).…”

Section: Discussionmentioning

confidence: 85%

Changes in the proteome of sea urchin Paracentrotus lividus coelomocytes in response to LPS injection into the body cavity

et al. 2020

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BackgroundThe immune system of echinoderm sea urchins is characterised by a high degree of complexity that is not completely understood. The Mediterranean sea urchin Paracentrotus lividus coelomocytes mediate immune responses through phagocytosis, encapsulation of nonself particles, and production of diffusible factors including antimicrobial molecules. Details of these processes, and molecular pathways driving these mechanisms, are still to be fully elucidated.

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Section: Discussionmentioning

confidence: 85%

Changes in the proteome of sea urchin Paracentrotus lividus coelomocytes in response to LPS injection into the body cavity

et al. 2020

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“…The primer sequences used for quantitative PCR (qPCR) were previously used: Pl-alx1 and Pl-tbr [22], Pl-fra1 [23], Pl-jun [20], Pl-foxO [25], Pl-pks1 and Pl-gcm [26]. The qPCR was run as reported by Chiaramonte et al [22]. Expression levels of the analysed genes are shown as fold change, compared to control embryos developed in DMSO/aMFSW without inhibitor, assumed as 1.…”

Section: Rna Isolation and Real-time Quantitative Pcrmentioning

confidence: 99%

Carbonic anhydrases in development: morphological observations and gene expression profiling in sea urchin embryos exposed to acetazolamide

et al. 2023

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Carbonic anhydrases (CANs) are conserved metalloenzymes catalysing the reversible hydration of carbon dioxide into protons and bicarbonate, with important roles in cells physiology. Some CAN-coding genes were found in sea urchin genome, although only one involved in embryonic skeletogenesis was described in Paracentrotus lividus . Here, we investigated gene expression patterns of P. lividus embryos cultured in the presence of acetazolamide (AZ), a CAN inhibitor, to combine morphological defects with their molecular underpinning. CAN inhibition blocked skeletogenesis, affected the spatial/temporal expression of some biomineralization-related genes, inhibited embryos swimming. A comparative analysis on the expression of 127 genes in control and 3 h/24 h AZ-treated embryos, using NanoString technology, showed the differential expression of genes encoding for structural/regulatory proteins, with different embryonic roles: biomineralization, transcriptional regulation, signalling, development and defence response. The study of the differentially expressed genes and the signalling pathways affected, besides in silico analyses and a speculative ‘interactomic model’, leads to predicting the presence of various CAN isoforms, possibly involved in different physiological processes/activities in sea urchin embryo, and their potential target genes/proteins. Our findings provide new valuable molecular data for further studies in several biological fields: developmental biology (biomineralization, axes patterning), cell differentiation (neural development) and drug toxicology (AZ effects on embryos/tissues).

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“…In addition to the SM genes, the MSP130 family of genes are a well-known component of echinoderm skeletogenesis (Figure 5). MSP130 is a cell-surface glycoprotein which is found in skeletogenic cells and skeletal tissues of echinoderms across classes and life history stages (Chiaramonte et al, 2020, Mann et al, 2008b, Livingston et al, 2006, Guss and Ettensohn, 1997a, Minokawa et al, 1997, Mann et al, 2010, Mann et al, 2008a. Homologues of MSP130 genes have been identified across numerous biomineralizing metazoans (Ettensohn, 2014, Szabó and Ferrier, 2015, Cameron and Bishop, 2012, Marie et al, 2011, as well as in the genomes, transcriptomes, and proteomes of crinoids, echinoids, ophiuroids and holothurians (Dylus et al, 2018, Livingston et al, 2006, Zhang et al, 2017, Li et al, 2020.…”

Section: Differentiation Gene Batteries In the Echinoderm Skeletogeni...mentioning

confidence: 99%

Molecular Paleobiology of the Echinoderm Skeleton

Thompson

2022

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The echinoderms are an ideal group to understand evolution from a holistic, interdisciplinary framework. The genetic regulatory networks underpinning development in echinoderms are some of the best known for any model group. Additionally, the echinoderms have an excellent fossil record, elucidating in in detail the evolutionary changes underpinning morphological evolution. In this Element, the echinoderms are discussed as a model group for molecular palaeobiological studies, integrating what is known of their development, genomes, and fossil record. Together, these insights shed light on the molecular and morphological evolution underpinning the vast biodiversity of echinoderms, and the animal kingdom more generally.

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PI3K inhibition highlights new molecular interactions involved in the skeletogenesis of Paracentrotus lividus embryos

Cited by 10 publications

References 56 publications

Changes in the proteome of sea urchin Paracentrotus lividus coelomocytes in response to LPS injection into the body cavity

Changes in the proteome of sea urchin Paracentrotus lividus coelomocytes in response to LPS injection into the body cavity

Carbonic anhydrases in development: morphological observations and gene expression profiling in sea urchin embryos exposed to acetazolamide

Molecular Paleobiology of the Echinoderm Skeleton

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