Phytotherapy in a rat model of hyperoxaluria: the antioxidant effects of quercetin involve serum paraoxonase 1 activation

Abstract

Serum paraoxonase 1 (PON1) has been reported to be an important contributor to the antioxidant and anti-inflammatory activities of HDL, avoiding LDL oxidation. The activity of this enzyme is reduced in patients with renal insufficiency, caused by elevated oxidative stress and disturbances of apolipoprotein metabolism. Therapeutic utilization of antioxidants to control renal oxidative stress may be an effective therapy in renal protection. The aim was to investigate the protective effects of several antioxidant compounds against the oxidative stress associated to renal failure induced by ethylene glycol (EG), focusing on the possible role of serum PON1 activity. Fifty-four male Wistar rats were randomly assigned to six groups (n = 9): an untreated control (C) group, an EG-treated group, a catechin (CAT)-treated group, an epicatechin (EPI)-treated group, a quercetin (QUE)-treated group and a folk herbal extract (FHE)-treated group. After 16 d of treatment, calcium oxalate lithiasis was induced in the rats using EG. After eight days (treatment + EG), the animals were sacrificed. EG treatment impaired kidney composition, increased oxidative damage, and decreased serum paraoxonase and arylesterase activities. CAT, QUE and the FHE Fagolitos improved oxidative status by enhancing antioxidant defenses - superoxide dismutase and PON1 activities - and reducing oxidative damage, thus reinforcing the idea of a possible role of PON1 in the protective effects of QUE against the deleterious consequences of oxidative stress in kidney.

Benfotiamine Enhances Antioxidant Defenses and Protects against Cisplatin‐Induced DNA Damage in Nephrotoxic Rats

“…The formation of a metal sulfhydryl complex decreases the activities of such enzymes . The decrease in PON1 activity by cisplatin treatment is in agreement with the study of Amengual‐Cladera et al., . PON1 has free thiol residues that are essential for its activity .…”

“…PON1 exerts paraoxonase and arylesterase activities, since the enzyme hydrolyzes both paraoxon and aromatic esters . The activity of PON1 is reduced in renal insufficiency caused by elevated oxidative stress .…”

Protective effect of Lysimachia christinae against acute alcohol-induced liver injury in mice

“…The main chemical components in L. christinae are flavonoids (37). As for the flavonoids in L. christinae, quercetin is relatively abundant (37) and has been found to have antioxidant activity in the previous study (38). However, it was still not clear until now that quercetin is the main hepatoprotective chemical compound of L. christinae.…”

Paraoxonase 1 in Chronic Kidney Failure

“…In a study with using the model of renal failure induced by ethylene glycol, it was reported that treatment impaired kidney composition, increased oxidative damage, and decreased serum paraoxonase and arylesterase activities [64]. Quercetin and cathechins enhanced antioxidant defenses—superoxide dismutase and PON1 activities, reducing oxidative damage suggesting that PON1 mediates the protective effects of flavonoids against kidney damage by oxidative stress [64].…”

“…Quercetin and cathechins enhanced antioxidant defenses—superoxide dismutase and PON1 activities, reducing oxidative damage suggesting that PON1 mediates the protective effects of flavonoids against kidney damage by oxidative stress [64]. …”