Phytoplankton Cell Counting by Flow Cytometry

Marie, Dominique; Simon, Nathalie; Vaulot, Daniel

doi:10.1016/b978-012088426-1/50018-4

Cited by 164 publications

(166 citation statements)

References 45 publications

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“…Data include light scatter and a variety of fluorescence signals (either natural fluorescence from pigments or from staining with fluorescent dyes or probes) emitted by that particle (see Chisholm et al 1986;Marie et al 2005;Olson et al 1991;Shapiro 2003). Generally, a laser exciting at 488 nm is used, although others, such as UV-emitting lasers, are also used (Binder et al 1996;Monger and Landry 1993).…”

Section: Distribution Abundance and Activitiesmentioning

confidence: 99%

Ecology and Diversity of Picoeukaryotes

Worden

Not

2008

Microbial Ecology of the Oceans

140

105

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Section: Distribution Abundance and Activitiesmentioning

confidence: 99%

Ecology and Diversity of Picoeukaryotes

Worden

Not

2008

Microbial Ecology of the Oceans

140

105

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“…Flow Cytometry analyses were performed with an Apogee-A40 flow cytometer (Apogee Flow Systems, UK) equipped with an argon laser (488 nm). Cells were counted following Marie et al (2005). Briefly, heterotrophic bacteria (HB) were detected by their signature in a plot of side scatter (SSC) versus green fluorescence (FL1).…”

Section: Size Fractionation Cell Counts and Species Identificationmentioning

confidence: 99%

“…The samples were later thawed, stained with SYBR-Green I (SYBR-I, 1:30 dilution of commercial stock; Invitrogen, USA; l ex λ 495 nm, l em λ 525 nm) diluted in dimethyl sulfoxide (DMSO, Merck, Germany) (Marie et al, 2005). Ten microliters of fluorochrome was added to 1 mL of bacterial sample.…”

Section: Size Fractionation Cell Counts and Species Identificationmentioning

confidence: 99%

Analysis of the hydrographic conditions and cyst beds in the San Jorge Gulf, Argentina, that favor dinoflagellate population development including toxigenic species and their toxins

Krock

Borel

Barrera

et al. 2015

Journal of Marine Systems

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The overlay of cooler nutrient enriched Beagle-Magellan water with warmer nutrient depleted shelf water and a strong stratification of the water column in the San Jorge Gulf region, Argentina, coincided with relatively high dinoflagellate abundances in April 2012, up to 34,000 cells L −1 . This dinoflagellate proliferation was dominated by Ceratium spp., but environmental conditions also favored to a lesser amount the occurrence of toxigenic dinoflagellates, such as Alexandrium tamarense and Protoceratium reticulatum, whose toxins were hardly detected in any other areas along the expedition transect of the R/V Puerto Deseado between 38 and 56°S (Ushuaia-Mar del Plata) in March/April 2012. Generally vegetative cells of A. tamarense and P. reticulatum co-occurred with their respective phycotoxins in the water column and their cysts in the upper sediment layers. Two strains of A. tamarense were isolated from the bloom sample and morphologically characterized. Their PSP toxin profiles consisted of C1/2, gonyautoxins 1/4 and to a lesser amount of neosaxitoxin and confirmed earlier data from this region. The ratios between autotrophic picoplankton and heterotrophic bacteria were higher in shelf waters in the north than in Beagle-Magellan waters in the south of San Jorge Gulf.

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“…As a drawback, epifluorescence microscopy is time consuming and limits the number of samples that can be processed. Flow cytometry (FC) counting has streamlined the assessment of the abundance and properties of prokaryotes (Gasol and del Giorgio, 2000), picophytoplankton (Marie et al, 2005) and HP as well, since the method optimization was presented years ago (Zubkov and Burkill, 2006;Zubkov et al, 2007) and refined recently (Christaki et al, 2011). However, this approach has not yet been applied routinely to large-scale oceanographic surveys.…”

Section: Introductionmentioning

confidence: 99%

Global abundance of planktonic heterotrophic protists in the deep ocean

et al. 2014

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The dark ocean is one of the largest biomes on Earth, with critical roles in organic matter remineralization and global carbon sequestration. Despite its recognized importance, little is known about some key microbial players, such as the community of heterotrophic protists (HP), which are likely the main consumers of prokaryotic biomass. To investigate this microbial component at a global scale, we determined their abundance and biomass in deepwater column samples from the Malaspina 2010 circumnavigation using a combination of epifluorescence microscopy and flow cytometry. HP were ubiquitously found at all depths investigated down to 4000 m. HP abundances decreased with depth, from an average of 72 ± 19 cells ml À 1 in mesopelagic waters down to 11 ± 1 cells ml À 1 in bathypelagic waters, whereas their total biomass decreased from 280±46 to 50±14 pg C ml À 1 . The parameters that better explained the variance of HP abundance were depth and prokaryote abundance, and to lesser extent oxygen concentration. The generally good correlation with prokaryotic abundance suggested active grazing of HP on prokaryotes. On a finer scale, the prokaryote:HP abundance ratio varied at a regional scale, and sites with the highest ratios exhibited a larger contribution of fungi molecular signal. Our study is a step forward towards determining the relationship between HP and their environment, unveiling their importance as players in the dark ocean's microbial food web.

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Phytoplankton Cell Counting by Flow Cytometry

Cited by 164 publications

References 45 publications

Ecology and Diversity of Picoeukaryotes

Ecology and Diversity of Picoeukaryotes

Analysis of the hydrographic conditions and cyst beds in the San Jorge Gulf, Argentina, that favor dinoflagellate population development including toxigenic species and their toxins

Global abundance of planktonic heterotrophic protists in the deep ocean

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