Phytohormone profiling of the sweet orange (Citrus sinensis (L.) Osbeck) leaves and roots using GC–MS-based method

Nehela, Yasser; Hijaz, Faraj; Elzaawely, Abdelnaser A.; El-Zahaby, Hassan M.; Killiny, Nabil

doi:10.1016/j.jplph.2016.04.005

Cited by 60 publications

(36 citation statements)

References 30 publications

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“…Compared with classic extraction methods, microextraction methods have the advantages of having high extraction efficiency and miniaturization of device and enrichment capacity making them more suitable for the analysis of plant hormone [47]. The extraction and clean-up techniques for trace compounds analysis include liquid-liquid extraction (LLE) [17,21,24,30,[63][64][65], liquid phase microextraction (LPME) [52,56,66], dispersive liquid-liquid microextraction (DLLME) [40,67], polymer monolith microextraction (PMME) [68], solid phase extraction (SPE) [26,38,55,[69][70][71][72], solid phase microextraction (SPME) [25,34], magnetic solid phase extraction (MSPE) [16,23,73], matrix solidphase dispersion (MSPD) [37], dispersive micro solid phase extraction (DMSPE) [74], ion pair stir bar sorptive extraction (SBSE) [1] and electromembrane extraction (EME) [75] (see Fig. 1).…”

Section: Common Pretreatment Methods In Plant Hormone Analysismentioning

confidence: 99%

“…Off-line [30] SA ABA Methanol-water-acetic acid extractionsolution (80: The application of SPME for in vivo analysis not only simplifies the extraction process, but also has the advantages of non-destructive extraction and easy to couple with mass spectrometry. Thus, this method facilitates onsite sampling, pretreatment and the detection of unstable analytes, making it suitable for long-term monitoring of signaling processes of plant hormones.…”

Section: Spme Strategies and Advancesmentioning

confidence: 99%

“…Particularly, combining gas chromatography (GC) or liquid chromatography (LC) with different MS detectors forms a rapid, sensitive and high-throughput method for quantitative analysis of plant hormone [21,[24][25][26]. Furthermore, in order to simultaneous analysis multi-class plant hormones in a single step, a derivatization step is often required before MS-based instrumental analysis because of the nonvolatile and non-ionizable property of some plant hormones [27][28][29][30][31][32]. Thus, derivatization step may promotes comprehensive investigation of the distribution of plant hormone among plant tissues, and hence characterization of metabolic and signaling processes involving plant hormones [33].…”

Section: Introductionmentioning

confidence: 99%

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Recent developments and emerging trends of mass spectrometric methods in plant hormone analysis: a review

et al. 2020

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Plant hormones are naturally occurring small molecule compounds which are present at trace amounts in plant. They play a pivotal role in the regulation of plant growth. The biological activity of plant hormones depends on their concentrations in the plant, thus, accurate determination of plant hormone is paramount. However, the complex plant matrix, wide polarity range and low concentration of plant hormones are the main hindrances to effective analyses of plant hormone even when state-of-the-art analytical techniques are employed. These factors substantially influence the accuracy of analytical results. So far, significant progress has been realized in the analysis of plant hormones, particularly in sample pretreatment techniques and mass spectrometric methods. This review describes the classic extraction and modern microextraction techniques used to analyze plant hormone. Advancements in solid phase microextraction (SPME) methods have been driven by the ever-increasing requirement for dynamic and in vivo identification of the spatial distribution of plant hormones in real-life plant samples, which would contribute greatly to the burgeoning field of plant hormone investigation. In this review, we describe advances in various aspects of mass spectrometry methods. Many fragmentation patterns are analyzed to provide the theoretical basis for the establishment of a mass spectral database for the analysis of plant hormones. We hope to provide a technical guide for further discovery of new plant hormones. More than 140 research studies on plant hormone published in the past decade are reviewed, with a particular emphasis on the recent advances in mass spectrometry and sample pretreatment techniques in the analysis of plant hormone. The potential progress for further research in plant hormones analysis is also highlighted.

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Section: Common Pretreatment Methods In Plant Hormone Analysismentioning

confidence: 99%

Section: Spme Strategies and Advancesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Recent developments and emerging trends of mass spectrometric methods in plant hormone analysis: a review

et al. 2020

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“…We centrifuged the remaining pellets again under the same rotation and collected the supernatants that were added to the respective microtubes. The microtubes were centrifuged at 14,000 g once again for 10 minutes at 4 ° C. From the supernatant of each sample was transferred 300 μL to a 2 mL vial [32,33].…”

Section: Biochemical Investigations On Leaves Attacked By Psyllids Rementioning

confidence: 99%

“…Afterwards, we used the MassHunter software to process the generated mass spectra and the Skyline software to make corrections of the areas and to determine concentration values in nanogram/gram (ng / g) [32,33]. We did all these procedures at the Núcleo de Análise de Biomoléculas (NuBioMol, CCB, UFV).…”

Section: Biochemical Investigations On Leaves Attacked By Psyllids Rementioning

confidence: 99%

The Psyllids Triozoida Limbata Supplant the Defenses of Psidium Guajava Plants to Garentee Feeding and Protection

Picanço

Carmo

Azevedo

et al. 2020

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Background: Herbivores cause biochemical, physiological, and morphological changes on plants. The ultimate effects of the herbivore activities are damages on the plant's body that can lead from local tissues death to the plant death. The intensity of herbivore attack and the plant's capability to defend itself determine the outcomes of herbivory. When the plant under attack has economic importance, such damages can diminish the productivity of the plantation and compromise the economy of families and the local communities. Although the adverse effects of herbivory on plants and economy are entirely known, some biological mechanisms underlying these damages remain unclear, especially at the anatomical and hormonal levels. The psyllid Triozoida limbata (Hemiptera: Triozidae) is a critical guava (Psidium guajava) pest in the Neotropical region, causing productivity plantation losses up to 55%. Psyllids cause visible morphological changes on guava leaves; however, there is a lack of detailed studied of the cellular, anatomical, and hormonal aspects of the subject. Thus, this work aimed to determine the alterations caused in guava leaves by T. limbata through anatomical and hormonal analyzes. Results: We found that the psyllid's nymphs feeding causes cells hypertrophy and hyperplasia determining the formation of the leaf-roll galls. Although there was no anatomical differentiation of the mesophilic tissues, the cells accumulate starch. Along with the anatomical changes, we observed increasing in the jasmonic acid and reduction of zeatin concentrations in the leaves. Conclusions: The alteration of phytohormone contents and the anatomical changes indicates that the feeding of T. limbata activates plant defenses, but it is not active toward the insects off. The insect nymphs live inside the leaf-roll galls until adults' phase, feeding on the guava leaves xylem and phloem. In commercial guava orchard, the control of T. limbata nymphs have been difficult. Thus, the understanding of the composition of this protective layer can help to find the most effective and efficient control solution for this pest.

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Effects of δ‐aminolevulinic acid dehydratase silencing on the primary and secondary metabolisms of citrus

et al. 2018

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δ‐aminolevulinic acid dehydratase (ALAD) is an important enzyme in tetrapyrrole synthesis. ALAD combines two δ‐aminolevulinic acid (δ‐ALA) molecules to form the pyrrole molecule, porphobilinogen, an important precursor for plant pigments involved in photosynthesis, respiration, and nutrient uptake. In this study, we investigated the effects of silencing of ALAD gene on citrus leaf pigments and metabolites. The ALAD enzyme was inhibited using virus‐induced gene silencing (VIGS) technology using citrus tristeza virus (CTV). δ‐ALA accumulated in citrus plants inoculated with the recombinant virus (CTV‐tALAD) to silence ALAD and resulted in discrete yellow spots (yellow islands) and necrosis in leaves and stems. The levels of chlorophylls, starch, sucrose, trans‐ and cis‐violaxanthin, and α‐ and β‐cryptoxanthin were reduced in CTV‐tALAD plants, whereas zeaxanthin was increased. The increase in zeaxanthin and the decrease in its precursors indicated that the reduction in chlorophylls resulted in light damage. Salicylic acid and jasmonic acid levels, as well as emission of (E)‐α‐bergamotene and (E)‐β‐farnesene, increased in CTV‐tALAD plants indicating these plants were under stress. Our results showed that silencing of ALAD induces stress in plants and that VIGS using mild CTV strains is a promising technique to study biological function of citrus genes.

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Phytohormone profiling of the sweet orange (Citrus sinensis (L.) Osbeck) leaves and roots using GC–MS-based method

Cited by 60 publications

References 30 publications

Recent developments and emerging trends of mass spectrometric methods in plant hormone analysis: a review

Recent developments and emerging trends of mass spectrometric methods in plant hormone analysis: a review

The Psyllids Triozoida Limbata Supplant the Defenses of Psidium Guajava Plants to Garentee Feeding and Protection

Effects of δ‐aminolevulinic acid dehydratase silencing on the primary and secondary metabolisms of citrus

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