Phytochemicals, Biological Activities, Molecular Mechanisms, and Future Prospects of <i>Plantago asiatica</i> L.

Wen, Shi-Yuan; Wei, Bingyan; Ma, Jieqiong; Wang, Li; Chen, Yanyan

doi:10.1021/acs.jafc.2c07735

Cited by 12 publications

(1 citation statement)

References 310 publications

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“…They also possess antioxidant properties and can improve inflammation caused by HP in the stomach. Plantago asiatica [ 41 ] is reported to have antioxidant, anti-inflammatory, and antibacterial effects. In vitro , Atractylodes lancea extracts inhibit HP NCTC11637 strain growth and biofilm formation[ 42 ].…”

Section: Discussionmentioning

confidence: 99%

Effect and mechanism of Qingre Huashi decoction on drug-resistant Helicobacter pylori

Lin,

Yang,

Huang

et al. 2024

World J Gastroenterol

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BACKGROUND Helicobacter pylori (HP), the most common pathogenic microorganism in the stomach, can induce inflammatory reactions in the gastric mucosa, causing chronic gastritis and even gastric cancer. HP infection affects over 4.4 billion people globally, with a worldwide infection rate of up to 50%. The multidrug resistance of HP poses a serious challenge to eradication. It has been de-monstrated that compared to bismuth quadruple therapy, Qingre Huashi decoction (QHD) combined with triple therapy exhibits comparable eradication rates but with a lower incidence of adverse reactions; in addition, QHD can directly inhibit and kill HP in vitro. AIM To explore the effect and mechanism of QHD on clinically multidrug-resistant and strong biofilm-forming HP. METHODS In this study, 12 HP strains were isolated in vitro after biopsy during gastroscopy of HP-infected patients. In vitro , the minimum inhibitory concentration (MIC) values for clinical HP strains and biofilm quantification were determined through the E-test method and crystal violet staining, respectively. The most robust biofilm-forming strain of HP was selected, and QHD was evaluated for its inhibitory and bactericidal effects on the strain with strong biofilm formation. This assessment was performed using agar dilution, E-test, killing dynamics, and transmission electron microscopy (TEM). The study also explored the impact of QHD on antibiotic resistance in these HP strains with strong biofilm formation. Crystalline violet method, scanning electron microscopy, laser confocal scanning microscopy, and (p)ppGpp chromatographic identification were employed to evaluate the effect of QHD on biofilm in strong biofilm-forming HP strains. The effect of QHD on biofilm and efflux pump-related gene expression was evaluated by quantitative polymerase chain reaction. Non-targeted metabolomics with UHPLC-MS/MS was used to identify potential metabolic pathways and biomarkers which were different between the NC and QHD groups. RESULTS HP could form biofilms of different degrees in vitro , and the intensity of formation was associated with the drug resistance of the strain. QHD had strong bacteriostatic and bactericidal effects on HP, with MICs of 32-64 mg/mL. QHD could inhibit the biofilm formation of the strong biofilm-forming HP strains, disrupt the biofilm structure, lower the accumulation of (p)ppGpp, decrease the expression of biofilm-related genes including LuxS , Spot , glup (HP1174), NapA , and CagE , and reduce the expression of efflux pump-related genes such as HP0605, HP0971, HP1327 , and HP1489 . Based on metabolomic analysis, QHD induced oxidative stress in HP, enhanced metabolism, and potentially inhibited relevant signaling pathways by upregulating adenosine monophosphate (AMP), thereby affecting HP growth, metabolism, and protein synthesis. CONCLUSION QHD exerts bacteriostatic and bactericidal effects on HP, and reduces HP drug resistance by inhibiting HP biofilm formation, destroying its biofilm structure, inhibiting the expression of biofilm-related genes and efflux pump-related genes, enhancing HP metabolism, and activating AMP in HP.

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Section: Discussionmentioning

confidence: 99%

Effect and mechanism of Qingre Huashi decoction on drug-resistant Helicobacter pylori

Lin,

Yang,

Huang

et al. 2024

World J Gastroenterol

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Geniposidic Acid Attenuates Chronic Tubulointerstitial Nephropathy Through Regulation of the NF‐ƙB/Nrf2 Pathway Via Aryl Hydrocarbon Receptor Signaling

Wang,

Li,

Wang

et al. 2024

Phytotherapy Research

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Renal fibrosis is an outcome of chronic kidney disease, independent of the underlying etiology. Renal fibrosis is caused primarily by oxidative stress and inflammation. We identified the components of Plantaginis semen and elucidated their anti‐fibrotic and anti‐inflammatory mechanisms. The renoprotective components and underlying molecular mechanisms of P. semen were investigated in rats with adenine‐induced chronic tubulointerstitial nephropathy (TIN) and in idole‐3‐acetic acid (IAA)–stimulated NRK‐52E cells. Acetate and n‐butanol extracts were found to be the bioactive fractions of P. semen. A total of 65 compounds including geniposidic acid (GPA), apigenin (APG), and acteoside (ATS) were isolated and identified. Among the seven main extract components, treatment with GPA, APG, and ATS reduced the serum levels of creatinine and urea in TIN rats. Mechanistically, GPA ameliorated renal fibrosis through repressing aryl hydrocarbon receptor (AHR) signaling and regulating redox signaling including inhibiting proinflammatory nuclear factor kappa B (NF‐ƙB) and its target gene products as well as activated antioxidative nuclear factor‐erythroid‐2‐related factor 2 (Nrf2) and its downstream target gene products in both TIN rats and IAA‐stimulated NRK‐52E cells. The inhibitory effect of GPA on AHR, NF‐Ƙb, and Nrf2 signaling were partially abolished in IAA‐stimulated NRK‐52E cells treated with CH223191 compared with untreated IAA‐stimulated NRK‐52E cells. These data demonstrated that GPA alleviates oxidative stress and inflammation partly by suppressing AHR signaling.

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Is the suppression of CD36 a promising way for atherosclerosis therapy?

Wen,

Zhi,

Liu

et al. 2024

Biochemical Pharmacology

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Phytochemicals, Biological Activities, Molecular Mechanisms, and Future Prospects of Plantago asiatica L.

Cited by 12 publications

References 310 publications

Effect and mechanism of Qingre Huashi decoction on drug-resistant Helicobacter pylori

Effect and mechanism of Qingre Huashi decoction on drug-resistant Helicobacter pylori

Geniposidic Acid Attenuates Chronic Tubulointerstitial Nephropathy Through Regulation of the NF‐ƙB/Nrf2 Pathway Via Aryl Hydrocarbon Receptor Signaling

Is the suppression of CD36 a promising way for atherosclerosis therapy?

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