Phytochemical study and anti-inflammatory and antioxidant potential of Spondias mombin leaves

Cabral, Bárbara; Siqueira, Emerson M.S.; Bitencourt, Mariana Angélica Oliveira; Lima, Maíra C.J.S.; Lima, Ana Karolina Oliveira Laurentino de; Ortmann, Caroline Flach; Chaves, Vítor Clasen; Fernandes-Pedrosa, Matheus de Freitas; Rocha, Hugo Alexandre Oliveira; Scortecci, Kátia Castanho; Reginatto, Flávio Henrique; Giordani, Raquel Brandt; Zucolotto, Silvana Maria

doi:10.1016/j.bjp.2016.02.002

Cited by 66 publications

(63 citation statements)

References 29 publications

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“…In folk medicine, various parts of the S. purpurea are used to treat gastric disorders, as antidiarrheals and as diuretics [ 17 , 21 , 22 ]. These activities may be related to the antioxidant properties already reported for this species and for the genus [ 23 , 24 ] and to the phenolic compound content present in S. purpurea [ 17 ].…”

Section: Introductionmentioning

confidence: 72%

Spondias purpurea L. (Anacardiaceae): Antioxidant and Antiulcer Activities of the Leaf Hexane Extract

Almeida

Brito

Santana

et al. 2017

Oxidative Medicine and Cellular Longevity

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Spondias purpurea is used in folk medicine to treat diarrhea and diuresis. The objective of this study was to evaluate the phytochemical profile and antioxidant and antiulcer activities of the hexane extract of the leaves of S. purpurea (SpHE). Phytochemical profile was evaluated via thin layer chromatography (TLC) and HPLC. SpHE was screened for antioxidant activities using DPPH, ABTS, FRAP, and phosphomolybdenum assays. To determine its antiulcer properties, animals were pretreated with injured control, lansoprazole, ranitidine, carbenoxolone, or SpHE (12.5, 25, and 50 mg/kg) and were screened; acute ulcers were induced by HCl/ethanol, absolute ethanol, and nonsteroidal anti-inflammatory drug (NSAID). TLC revealed the presence of flavonoids, whereas HPLC analysis showed the presence of caffeic acid and epigallocatechin. The phenolic compounds and in vitro assays showed antioxidant activity. After gastric ulcer induction by using HCl/ethanol, SpHE reduced the area of ulcerative lesions by 82, 91, and 88%, respectively. In ethanol, SpHE reduced the area of ulcerative lesions by 77, 93, and 92%, respectively. In the NSAID, the percentages of protection were 70, 76, and 78%, respectively. SpHE promoted the minimization of ulcers, increased the levels of reduced glutathione, and decreased tumor necrosis factor. S. purpurea has antioxidant and antiulcer properties.

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Section: Introductionmentioning

confidence: 72%

Spondias purpurea L. (Anacardiaceae): Antioxidant and Antiulcer Activities of the Leaf Hexane Extract

Almeida

Brito

Santana

et al. 2017

Oxidative Medicine and Cellular Longevity

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“…mombin L. The fruits of S . mombin L. are known by many different popular names, the best known of which in Brazil are cajá, cajá-mirim, and taperebá [7, 8]. …”

Section: Introductionmentioning

confidence: 99%

Evaluation of gastroprotective and ulcer healing activities of yellow mombin juice from Spondias mombin L.

et al. 2018

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Spondias mombin L. (yellow mombin) is a tree with a nutritional fruit that is commonly consumed in the North and Northeast of Brazil, as the juice of its pulp is rich in antioxidant compounds. This study aimed to investigate the gastroprotective and ulcer healing activities of yellow mombin juice (YMJ) in Wistar rats, and to elucidate the possible involved mechanisms. Phytochemical characterization of the lyophilized fruit juice was performed by high-performance liquid chromatography (HPLC). The gastroprotective activity of YMJ was investigated in ethanol (25, 50, and 100% YMJ) and indomethacin (100% YMJ) models of acute gastric ulcer in rats. Then, the effect of YMJ on mucus production and gastric secretions, and the involvement of non-protein sulfhydryl groups and prostaglandins in the gastroprotective process were examined. Moreover, the ulcer healing effect of YMJ was investigated in a model of acetic acid-induced chronic ulcer through histological and immunohistochemical analyses. HPLC results identified the presence of epicatechin (7.1 ± 1.6 μg/mL) and quercetin (17.3 ± 2.5 μg/mL) in YMJ. Ethanol-induced gastric lesions were inhibited by YMJ (25, 50, and 100%) by 42.42, 45.09, and 98.21% respectively, and indomethacin-induced lesions were inhibited by YMJ (100%) by 58.96%, compared to control group. Moreover, YMJ reduced gastric content and total acidy by 57.35 and 71.97%, respectively, compared to the control group. Treatment with YMJ also promoted healing of chronic ulcer, regeneration of the gastric mucosa, and restoration of mucus levels in glandular cells, as confirmed by histological analysis. It also increased cellular proliferation, as demonstrated by high reactivity to Ki-67 and bromodeoxyuridine. In conclusion, YMJ was found to possess gastroprotective and ulcer healing activities that are correlated to its antisecretory action. These results support the commercial exploration of YMJ as a functional food.

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“…Geraniin has already been identified in Spondias mombin leaves extract [15]. Other phenolic compounds, such as chlorogenic acid, derivatives of caffeic acid, ellagic acid, rutin, isoquercitrin, and quercetin, have been identified in different leaves extracts of this species [14,18,30].…”

Section: Resultsmentioning

confidence: 97%

“…The plant is popularly known as cajá, taperebá, and cajá-mirim, among others [12,13]. Extracts from S. mombin leaves have exhibited antiviral, antimicrobial, anti-inflammatory and antioxidant activities in previous studies [14][15][16]. Literature has reported that S. mombin leaves are active in in vitro antiviral assays against HSV-1 [15].…”

Section: Introductionmentioning

confidence: 99%

“…Additionally, it is necessary to standardize and/or quantify the chemical markers in the extract in order to develop an herbal product with S. mombin [17]. The presence of flavonoids, phenolic acids and tannins have already been identified in the extract of S. mombin leaves [14,18], such as geraniin, an ellagitannin with previous evidence of in vitro antiviral activity [15,19].…”

Section: Introductionmentioning

confidence: 99%

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Antiviral Potential of Spondias mombin L. Leaves Extract Against Herpes Simplex Virus Type-1 Replication Using In Vitro and In Silico Approaches

et al. 2020

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Spondias mobin leaves have been traditionally used for treating cold sores. The study investigated the mechanism of antiherpes action of S. mombin extract, fractions, and geraniin. Different concentrations of samples were used to evaluate the in vitro antiherpes activity (anti-HSV-1) in virucidal, post-infection, attachment, and penetration assays. The mechanism of action of geraniin was investigated considering the glycoproteins gB and gD of HSV-1 surface as potential molecular targets. Molecular docking simulations were carried out for both in order to determine the possible binding mode position of geraniin at the activity sites. The binding mode position was posteriorly optimized considering the flexibility of the glycoproteins. The chemical analysis of samples was performed by LC-MS and revealed the presence of 22 substances, which are hydrolysable tannins, O-glycosylated flavonoids, phenolic acids, and a carbohydrate. The extract, tannin-rich fraction and geraniin showed important in vitro virucidal activity through blocking viral attachment but showed no relevant inhibition of viral penetration. The in silico approaches demonstrated a high number of potential strong intermolecular interactions as hydrogen bonds between geraniin and the activity site of the glycoproteins, particularly the glycoprotein gB. In silico experiments indicated that geraniin is at least partially responsible for the anti-herpes activity through interaction with the viral surface glycoprotein gB, which is responsible for viral adsorption. These results highlight the therapeutic potential of S. mombin anti-herpes treatment and provides support for its traditional purposes. However, further studies are required to validate the antiviral activities in vivo, as well as efficacy in humans.

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Phytochemical study and anti-inflammatory and antioxidant potential of Spondias mombin leaves

Cited by 66 publications

References 29 publications

Spondias purpurea L. (Anacardiaceae): Antioxidant and Antiulcer Activities of the Leaf Hexane Extract

Spondias purpurea L. (Anacardiaceae): Antioxidant and Antiulcer Activities of the Leaf Hexane Extract

Evaluation of gastroprotective and ulcer healing activities of yellow mombin juice from Spondias mombin L.

Antiviral Potential of Spondias mombin L. Leaves Extract Against Herpes Simplex Virus Type-1 Replication Using In Vitro and In Silico Approaches

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