Phytochemical screening of different Crataegus Oxyacantha extracts

Abstract

ABSTRACT. Crataegus oxyacantha L., the hawthorn, is a well-known medicinal plant with cardiotonic effect. This paper presents a complex phytochemical screening of polyphenols profile, both qualitatively and quantitatively, in three different extracts obtained from fresh berries, flowers with leaves and young shoots. There were used spectral (UV-Vis) and chromatographic (TLC, HPLC) methods to evaluate the total flavonoids, total phenolic acids and qualitative polyphenols profile of the extracts. The antioxidant capacity was evaluated by DPPH, FRAP and NO radical inhibition methods, by UV-Vis spectrophotometry. The total flavonoid content expressed in rutoside ranges from 0.49 to 1.33 mg/ml, the total phenolic acids expressed in caffeic acid from 0.02 to 0.10 mg/ml, the most concentrated being the berries extract. In flowers with leaves extract can be identified and quantified a special flavonoid, the vitexine, 0.37 mg/ml. All three extracts present important antioxidant activity, both on ROS and RNS species. By FRAP method the berries extract was determined as most powerful, having also the best inhibition effect on NO radicals. The DPPH method indicates the special young shoots extract, used in gemmotherapy, as being the most powerful, with significant inhibiting effect on NO radicals. This indicates that the young shoots gemmotherapic extract is a good candidate for a powerful therapeutic tool.

Food Applications and Potential Health Benefits of Hawthorn

“…Flavonoids are the most abundant and wide-ranging class of compounds in hawthorn [ 25 , 26 ]. Currently, more than 60 flavonoids have been isolated from hawthorn [ 22 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 ], including the oxidized (chrysin, rutin, and quercetin) and reduced (proanthocyanidins, i.e., catechin derivatives) types [ 35 ].…”

Teratogen Potential Evaluation of the Aqueous and Hydroalcoholic Leaf Extracts of Crataegus oxyacantha in Pregnancy Rats

“…Used since ancient times mainly in treating cardiovascular diseases [ 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 ], its activity has been described as lipid-lowering [ 24 , 25 , 26 ], immunomodulatory [ 27 , 28 ], hepatoprotective [ 29 , 30 , 31 ], anti-inflammatory [ 32 , 33 ], antioxidant [ 32 , 33 , 34 , 35 , 36 , 37 ], antimicrobial [ 33 , 37 , 38 ], anxiolytic, and antidepressant [ 39 , 40 ]. These activities have been associated with the different types of flavonoids that are present in the leaf, bark, fruit, and flowers of C. oxyacantha .…”

Cytotoxic and Genotoxic Evaluation of the Aqueous and Hydroalcoholic Leaf and Bark Extracts of Crataegus oxyacantha in Murine Model

“…Several studies carried out in recent decades describe the anti-inflammatory [ 3 , 4 , 5 ], gastroprotective [ 4 , 5 ], antimicrobial [ 4 , 5 , 6 , 7 , 8 ], hypolipidemic [ 5 , 9 , 10 , 11 , 12 ], immunomodulatory [ 5 , 13 , 14 ], antioxidant [ 2 , 5 , 15 , 16 , 17 , 18 , 19 ], and cardioprotective [ 2 , 3 , 5 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 ] properties of C. oxyacantha .…”