Phytochemical Screening, Antibacterial, Antifungal, Antiviral, Cytotoxic, and Anti-Quorum-Sensing Properties of Teucrium polium L. Aerial Parts Methanolic Extract

Abstract

The chemical profile of Teucrium polium L. (T. polium) methanolic extract was tested using liquid chromatography coupled with high resolution mass spectrometry (HR-LCMS). Disc diffusion and microdilution assays were used for the antimicrobial activities. Coxsackievirus B-3 (CVB3) and Herpes simplex virus type 2 (HSV-2) were used for the antiviral activities. Chromobacterium violaceum (ATCC 12472 and CV026) and Pseudomonas aeruginosa PAO1 were used as starter strains for the anti-quorum sensing tests. Isoprenoids are the main class of compounds identified, and 13R-hydroxy-9E,11Z-octadecadienoic acid, valtratum, rhoifolin, sericetin diacetate, and dihydrosamidin were the dominant phytoconstituents. The highest mean diameter of growth inhibition zone was recorded for Acinetobacter baumannii (19.33 ± 1.15 mm). The minimal inhibitory concentrations were ranging from 6.25 to 25 mg/mL for bacterial strains, and from 6.25 to 25 mg/mL for Candida species. The 50% cytotoxic concentration on VERO (African Green Monkey Kidney) cell lines was estimated at 209 µg/mL. No antiviral activity was recorded. Additionally, T. polium extract was able to inhibit P. aeruginosa PAO1 motility in a concentration-dependent manner. However, the tested extract was able to inhibit 23.66% of the swarming and 35.25% of swimming capacities of PAO1 at 100 µg/mL. These results highlighted the role of germander as a potent antimicrobial agent that can interfere with the virulence factors controlled by the quorum-sensing systems.

Teucrium polium (L.): Phytochemical Screening and Biological Activities at Different Phenological Stages

“…It has been reported that T. polium compounds have anti-profilative, anti-diabetic, anticancer, and pro-apoptotic activities [ 15 , 29 ]. Recently, a lot of studies, focusing on the biological and pharmacological activity of plants in folk medicine, including the plant of Teucrium polium L., mostly have indicated significant differences in chemical composition (total flavonoids, total phenolic contents) and biological activity due to differences in the geographical location of the plant material but not differences due to its phenological stage [ 30 , 31 ].…”

Phytochemistry, Bioactivities, Pharmacokinetics and Toxicity Prediction of Selaginella repanda with Its Anticancer Potential against Human Lung, Breast and Colorectal Carcinoma Cell Lines

“…Several examples of plant-based drugs include nicotine (antimicrobial), nordihydroguaiaretic acid (antioxidant), vinblastine (anticancer), aescin (anti-inflammatory), acetyldigoxin (cardiotonic), l -dopa (antiparkinsonism), etc. In spite of the discovery of innumerable drugs of plant origin, the exploration of novel bioactive compounds is yet requisite to enhance the range access and to search less toxic and more efficacious drugs [ 4 , 5 , 6 ]. Medicinal and economic importance of higher plants, specifically angiosperms, has been explored thoroughly.…”

Anti-Adhesion and Antibiofilm Activity of Eruca sativa Miller Extract Targeting Cell Adhesion Proteins of Food-Borne Bacteria as a Potential Mechanism: Combined In Vitro-In Silico Approach

“…In recent years, antimicrobial agents derived from different natural resources gained higher interest. Amongst medicinal plants are the most important as they possess diverse type of phytochemicals, which can significantly target different sites or pathways of the bacterial pathogens hence stop or inhibiting the growth of pathogens [ 12 , 24 , 25 , 26 , 27 , 28 , 29 ]. Numerous biologically active molecules from plants proffer a repository of antimicrobial compounds and have drawn substantial research interest.…”