Phytochemical Profile of Asplenium aethiopicum (Burm. f.) Becherer Using HPTLC

Abstract

The present study was aimed to validate the phenolic, flavonoids, alkaloids and tannins profile of Aspleniuma ethiopicum (Burm. f.) Becherer methanolic extracts using HPTLC (High-performance thin-layer chromatography). The chromatographic separation was performed using the standard method. The separation methodology was optimized and phytoconstituents of A. aethiopicum methanolic extracts were separated. The methanolic extract of A. aethiopicum showed several bands identified as known phenolic compounds. The obtained flavonoids profiles were identified. HPTLC alkaloids profile of A. aethiopicum revealed 11 types of alkaloids. The tannin profile of methanolic extracts of A. aethiopicum demonstrated 11 different types of tannins. The observed profiles will be used as phytomarker to identify the chemical constituents of A. aethiopicum methanolic extracts. These profiles will act as a fingerprint of A. aethiopicum and help us to distinguish from its adulterants. The observed profile will help us to identify the crude drugs and improve the therapeutic potentials of A. aethiopicum.

Applications of Chromatographic Techniques in Food and Environmental Analysis

“…Different techniques were applied for the characterization of food and plants with nutritional or pharmaceutical interest. In this sense, it is worth mentioning the work of Antonysamy Johnson et al [6], who used HPTLC combined with UV detection for the phytochemical characterization of Asplenium aethiopicum in order to evaluate the potential therapeutic character of this plant. The results helped to identify phenolics, tannins, alkaloids, and flavonoid profiles that confirmed the antioxidant and cytotoxicity potential of this plant and, additionally, provided a powerful tool for their identification and differentiation from other adulterants, which could be used with the same aim.…”