Phytochemical and Pharmacognostic Study of Catharnthus Roseus Using Deep Eutectic Solvents

Abstract

Medicinal plant derived compound have an important role in the development of many clinically useful agents. Catharanthus roseus is a significant medicinal plant of the Apocynaceae family. Catharanthus roseus plant is used for different disease treatment such as skin cancer, Hodgkin's disease, breast cancer, and lymphoblastic leukemia. The aim of this study is conferring the comparative abundances of different phytochemicals, physiochemical and fluorescence analysis from Catharanthus roseus. Some of the chemical constituent's tests have been performed for phytochemical analysis. Fluorescence analysis was done by using dried powder mixed with different chemicals and have been observed under UV light (365 nm) and ordinary light. Total ash, acid insoluble ash, and moisture have been selected for physiochemical analysis. The precipitate formation or colour change was used as analytical answers for phytochemical analysis. The result of fluorescence analysis observed under UV light (365nm) and ordinary light by observing colour changes in sample. The percentage of loss of weight on drying, total ash and acid insoluble ash were obtained by employing standard methods of analysis for the physiochemical test. Extraction of Catharanthus roseus plant using deep eutectic solvent gave positive results.

Biological isolation and characterization of Catharanthus roseus (L.) G. Don methanolic leaves extracts and their assessment for antimicrobial, cytotoxic, and apoptotic activities

“…The phytochemicals present in methanolic leaf extracts of the C. roseus plants had exposed the existence of seven phytochemicals for example alkaloids, carbohydrates, flavonoids, glycosides, phenols, steroids as well as tannins indicating that the rich presence of auxiliary metabolites. This is in understanding that of prior investigations by different workers [21,22,40,41,63]. Comparable phytoconstituents present in the raw extracts were recorded in the C. roseus with different plant parts like leaves by Govindasamy and Srinivasan [17] and Wagay et al [63], stem by Pham et al [49], flower by Govindasamy and Srinivasan [17], root by Govindasamy and Srinivasan [17], and stem bark by Rose and Priya [55].…”

Biological Isolation and Characterization of Catharanthus roseus (L.) G. Don Methanolic Leaf Extracts and their Assessment of Antimicrobial, Cytotoxic and Apoptotic Activities

“…The methanolic extract of C. roseus plant produces the typical light blue colour when seen under UV light (365 nm), and it was contrasted with colours oscillated from brown to green observed under visible light. This is in accordance with the previous discoveries of Nisar et al (2016aNisar et al ( , 2016b. It is very utilized in the biochemical investigation, for example, partition or seclusion of plant metabolites from the methanolic extracts of C. roseus plants.…”

“…The phytochemicals present in the methanolic leaf extracts of the C. roseus plants had exposed the existence of seven phytochemicals such as alkaloids, carbohydrates, avonoids, glycosides, phenols, steroids and tannins indicate that the rich presence of auxiliary metabolites. This is in understanding that of prior investigations by different workers (Wagay et al 2013;Kabesh et al 2015;Nisar et al 2016aNisar et al , 2016bKale et al 2018). Comparable phytoconstituents present in the raw extracts were recorded in the C. roseus with different plant parts like leaves by Govindasamy and Srinivasan (2012) and Wagay et al (2013), stem by Pham et al (2018), ower by Govindasamy and Srinivasan (2012), root by Govindasamy and Srinivasan (2012), and stem bark by Rose and Priya (2017).…”

“…Catharanthus roseus is one of the signi cant therapeutic plants, very much perceived in Ayurveda and other customary treatment system. It is recognized for its anti-tumour, anti-diabetic, anti-microbial, anti-oxidant and anti-mutagenic effects (Nisar et al 2016a(Nisar et al , 2016b. As per Govindasamy and Srinivasan (2012), the antibacterial, antitumor, anti-in ammatory and antimicrobial properties of the plant extracts are because of the presence of alkaloids.…”

Deep Eutectic Solvents: An Overview of its Application as a “Green” Extractant

“…However, it was found that there are little available information with regards to the polarity of DESs in the literature. In their studies, Nisar et al, [45] have used deep eutectic solvent composed of choline chloride and glycerol to extract some phytochemicals from Catharanthus roseus roots, stems, leaves, and flower petal. The results indicated the presence of many active chemical constituents including alkaloids, saponins, steroids, terpenoids, phenol, flavanoids and reducing sugar.…”