Phytochemical Analysis and Free Radical Scavenging Activity of Medicinal Plants Gnidia glauca and Dioscorea bulbifera

Abstract

Gnidia glauca and Dioscorea bulbifera are traditional medicinal plants that can be considered as sources of natural antioxidants. Herein we report the phytochemical analysis and free radical scavenging activity of their sequential extracts. Phenolic and flavonoid content were determined. Scavenging activity was checked against pulse radiolysis generated ABTS•+ and OH radical, in addition to DPPH, superoxide and hydroxyl radicals by biochemical methods followed by principal component analysis. G. glauca leaf extracts were rich in phenolic and flavonoid content. Ethyl acetate extract of D. bulbifera bulbs and methanol extract of G. glauca stem exhibited excellent scavenging of pulse radiolysis generated ABTS•+ radical with a second order rate constant of 2.33×106 and 1.72×106, respectively. Similarly, methanol extract of G. glauca flower and ethyl acetate extract of D. bulbifera bulb with second order rate constants of 4.48×106 and 4.46×106 were found to be potent scavengers of pulse radiolysis generated OH radical. G. glauca leaf and stem showed excellent reducing activity and free radical scavenging activity. HPTLC fingerprinting, carried out in mobile phase, chloroform: toluene: ethanol (4: 4: 1, v/v) showed presence of florescent compound at 366 nm as well as UV active compound at 254 nm. GC-TOF-MS analysis revealed the predominance of diphenyl sulfone as major compound in G. glauca. Significant levels of n-hexadecanoic acid and octadecanoic acid were also present. Diosgenin (C27H42O3) and diosgenin (3á,25R) acetate were present as major phytoconstituents in the extracts of D. bulbifera. G. glauca and D. bulbifera contain significant amounts of phytochemicals with antioxidative properties that can be exploited as a potential source for herbal remedy for oxidative stress induced diseases. These results rationalize further investigation in the potential discovery of new natural bioactive principles from these two important medicinal plants.

High-Performance Thin Layer Chromatography (Hptlc) Fingerprinting Pattern of Mangrove Avicennia Marina

“…The mobile phase which was optimized for this study was also previously used in some very recent studies related to the characterization and analysis the phytochemistry of medicinal plants [42][43], comparison of HPTLC pattern assessment of plants [44][45] also with slightly modifications in selected mobile phase [46], study the autograph and drug formulation [47] as well as quantification of specific bioactive compound [48] and produce fingerprints [49]. Here this mobile phase gave very reasonable Rf and resolution of the separated bands and capable of generating a specific fingerprint for leaf, stem, and root parts of this mangrove separately in a quick/single run.…”

Endophytes from medicinal plants and their potential for producing indole acetic acid, improving seed germination and mitigating oxidative stress

“…A superoxide anion radical scavenging assay was executed for the endophytic cultures as described by Ghosh et al (2013) using non-enzymatic phenazine methosulfate-nicotinamide adenine dinucleotide (PMS-NADH) system. The samples' superoxide anion scavenging activities measured at 560 nm were calculated according to the equation: percentage scavenging (%)=1−(absorbance of sample at 560 nm/ absorbance of control at 560 nm)×100%.…”

Novel platinum–palladium bimetallic nanoparticles synthesized by Dioscorea bulbifera: anticancer and antioxidant activities

“…[24][25][26][27] Similarly, it has been extensively used for synthesis of metal nanoparticles like gold, silver, and gold core silver shell (Au core Ag shell NPs) bimetallic nanoparticles. [28][29][30] Hereby, as a part of our growing interest in nanobiotechnology, herein we report for the first time, the synthesis of Pt-PdNPs by a medicinal plant, D. bulbifera.…”

“…24 In short, 100 µL of PtNPs, PdNPs, and Pt-PdNPs were added separately to 400 µL of phosphate buffer, followed by addition of 100 µL of ethylenediaminetetraacetic acid (1.04 mM), 100 µL of FeCl 3 (1.0 mM), and 100 µL of 2-deoxyribose (60 mM). Initiation of reaction was achieved by addition of 100 µL of ascorbic acid (2 mM) and 100 µL of H 2 O 2 (10 mM) into the mixture keeping in water bath at 37°C.…”