Phytochemical analysis and antioxidant properties of organic extracts obtained from Cynoglossum cheirifolium L.

Bensaid, Ilhem; Bekkara, Fawzia Atik; Haci, Imad Abdelhamid El; Belarbi, Karima; Beddou, Fawzia; Bekhechi, Chahrazed

doi:10.1007/s13596-017-0291-9

Cited by 6 publications

(2 citation statements)

References 21 publications

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“…However, the positive effects may vary according to the species of the plant and its used part. It is common to find studies that demonstrate, through different chromatographic analyses, different concentrations of flavonoids, tannins, and triterpenes in the bark and leaves of the same plant species [82–84], and that the protective effect of an extract should consider the part of the plant, possibly associated with its antioxidant effect [85]. In this review, we observed that approximately a quarter of the studies do not provide the scientific name of the species or the part of the plant used, which reduces the accuracy and reproducibility of these studies.…”

Section: Discussionmentioning

confidence: 99%

Plant Extracts in the Bone Repair Process: A Systematic Review

Miranda

Guimarães-Lopes

Altoé

et al. 2019

Mediators of Inflammation

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Bone lesions are an important public health problem, with high socioeconomic costs. Bone tissue repair is coordinated by an inflammatory dynamic process mediated by osteoprogenitor cells of the periosteum and endosteum, responsible for the formation of a new bone matrix. Studies using antioxidant products from plants for bone lesion treatment have been growing worldwide. We developed a systematic review to compile the results of works with animal models investigating the anti-inflammatory activity of plant extracts in the treatment of bone lesions and analyze the methodological quality of the studies on this subject. Studies were selected in the PubMed/MEDLINE, Scopus, and Web of Science databases according to the PRISMA statement. The research filters were constructed using three parameters: animal model, bone repair, and plant extracts. 31 full-text articles were recovered from 10 countries. Phytochemical prospecting was reported in 15 studies (48.39%). The most common secondary metabolites were flavonoids, cited in 32.26% studies (n = 10). Essential criteria to in vivo animal studies were frequently underreported, suggesting publication bias. The animals treated with plant extracts presented positive results in the osteoblastic proliferation, and consequently, this treatment accelerated osteogenic differentiation and bone callus formation, as well as bone fracture repair. Possibly, these results are associated with antioxidant, regenerative, and anti-inflammatory power of the extracts. The absence or incomplete characterization of the animal models, treatment protocols, and phytochemical and toxicity analyses impairs the internal validity of the evidence, making it difficult to determine the effectiveness and safety of plant-derived products in bone repair.

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Section: Discussionmentioning

confidence: 99%

Plant Extracts in the Bone Repair Process: A Systematic Review

Miranda

Guimarães-Lopes

Altoé

et al. 2019

Mediators of Inflammation

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“…The observed antioxidant activity of the methanolic extract from Cynoglossum tubiflorus leaves can be attributed to its abundant content of phenolic compounds and flavonoids, as suggested by previous studies [ 42 ]. In a previous research investigation, Ilhem et al worked on a species from the genus Cynoglossum and reported good antioxidant activity for all the tested fractions, with IC50s ranging from 0.078 to 0.483 mg/mL [ 43 ]. However, in comparison with these results, Cynoglossum tubiflorus was found to be even more active in terms of antioxidant activity due to its richness in phenolic compounds, especially flavonoids.…”

Section: Resultsmentioning

confidence: 99%

HPLC-HESI-MS/MS Analysis of Phenolic Compounds from Cynoglossum tubiflorus Leaf Extracts: An Assessment of Their Cytotoxic, Antioxidant, and Antibacterial Properties

Dallali,

Fakhfakh,

Paris

et al. 2024

Plants

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The current study aimed to investigate the chemical composition, antioxidant, antibacterial, and cytotoxic properties of three extracts (hexane, dichloromethane, and methanol) from Cynoglossum tubiflorus. The composition of the methanolic extract was elucidated using HPLC-HESI-MS/MS analysis. The antioxidant effect was examined using NO, DPPH, FRAP, and TAC assays. Antimicrobial activity was evaluated by broth microdilution using various bacterial strains such as S. aureus, S. epidermidis, P. aeruginosa, E. coli, and K. pneumoniae. Structural disruptions in Gram-positive bacteria were visualized using scanning electron microscopy (SEM). Cytotoxic effects were evaluated on human MRC-5 in culture according to the MTT assay. The outcomes suggest that methanol extract contained a high amount of phenolic compounds (254.35 ± 0.360 mg GAE/g DE and 211.59 ± 0.939 mg QE/g DE). By applying the HPLC-HESI-MS/MS analysis, 32 compounds were identified, including phenolic acids, flavonoids, lignans, and fatty acids. This extract showed strong antioxidant (IC50 = 0.043 ± 0.001 mg/mL) and antimicrobial (MIC = 156 µg/mL) activities. The SEM suggests that cells exhibited membrane distortions characterized by surface depressions and alterations in bacterial shape, including dents, when compared to untreated cells. The in vitro cytotoxicity effect on human MRC-5 cells showed no toxicity effects at a concentration of 600 µg/mL. In silico analysis predicted low toxicity for all tested compounds across four different administration routes. This research indicates that this plant could be explored as a powerful source of natural drugs to target pathogens, with applications in the food, pharmaceutical, and medical industries.

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