Phytochemical Analysis and Antioxidant, Antimicrobial, and Antiaging Activities of Ethanolic Seed Extracts of Four Mucuna Species

Theansungnoen, Tinnakorn; Nitthikan, Nichcha; Wilai, Mayuramas; Chaiwut, Phanuphong; Kiattisin, Kanokwan; Intharuksa, Aekkhaluck

doi:10.3390/cosmetics9010014

Cited by 20 publications

(15 citation statements)

References 61 publications

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“…The flow rate was 0.1 µL/min, and detection was performed at 210 nm. L-DOPA analysis of MW, ME, and MA was identified by comparing the retention time of L-DOPA, a standard material, under the same analysis conditions [18,19].…”

Section: Ultra-performance Liquid Chromatography (Uplc)mentioning

confidence: 99%

Anti-Inflammatory Activity of Velvet Bean (Mucuna pruriens) Substances in LPS−Stimulated RAW 264.7 Macrophages

Han

2022

Molecules

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In this study, Mucuna pruriens extracts were used to verify their application as a natural-based raw material with anti-inflammatory function. A nitric oxide inhibition activity assay showed that M. pruriens extracted with hot water (MW), M. pruriens extracted with 70% ethanol (ME), and M. pruriens extracted with 70% acetone (MA) presented NO inhibition activity; among them, MW and ME demonstrated the best activity and were selected for Western blot analysis. After identifying the expression patterns of inflammation-related proteins, such as inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), c-jun N-terminal kinase (JNK), extracellular signal-regulated kinase (ERK), and nuclear factor kappa-light-chain-enhancer of activated B cell (NF-κB), through Western blots, both MW and ME showed inhibition patterns. As a result of analyzing L-DOPA contained in M. pruriens through ultra-performance liquid chromatography (UPLC), high L-DOPA content was detected in MW, ME, and MA. Therefore, it can be concluded that M. pruriens extracts have the potential for use as an anti-inflammatory material.

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Section: Ultra-performance Liquid Chromatography (Uplc)mentioning

confidence: 99%

Anti-Inflammatory Activity of Velvet Bean (Mucuna pruriens) Substances in LPS−Stimulated RAW 264.7 Macrophages

Han

2022

Molecules

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“…Jimoh et al reported that M. pruriens seed aqueous extract contained 12 mg GAE/g extract and 3.18 mg QE/g extract [ 31 ]. Theansungnoen et al reported that the total phenolic compounds in Thai mucuna seed extract was 21.78 ± 3.58 mg GAE/g extract and total flavonoid content found in this plant seed was 423.48 ± 6.71 mg QE/g extract [ 32 ].…”

Section: Resultsmentioning

confidence: 99%

Development of Jelly Loaded with Nanogel Containing Natural L-Dopa from Mucuna pruriens Seed Extract for Neuroprotection in Parkinson’s Disease

et al. 2022

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The first line therapy of patients with Parkinson’s disease, a neurodegenerative disorder caused by the degeneration of dopaminergic neurons, is levodopa (L-dopa) given orally. Recently, the presence of natural L-dopa in the seed of Mucuna pruriens, a tropical legume in the Fabaceae family, was reported and it showed superior efficiency compared with synthetic L-dopa. Therefore, this study aimed to examine the phytochemical compounds, particularly for natural L-dopa, in M. pruriens seed extract and subsequently prepare a nanogel containing the extract prior to incorporation into a jelly formulation for use as a functional food in elderly patients with Parkinson’s disease. The results show that M. pruriens seed extract contains phenolic compounds, flavonoids, tannins, alkaloids, terpenoids, and saponins. The quantitative analysis performed by the HPLC method revealed that spray-dried M. pruriens seed extract contained 5.59 ± 0.21% L-dopa. M. pruriens seed extract possesses a ferric-reducing antioxidant power and shows free-radical scavenging activity, determined by DPPH and ABTS methods, suggesting a distinctive antioxidant ability of the extract. M. pruriens seed extract at 10 ng/mL did not show cytotoxicity against a neuronal cell line (SH-SY5Y cells), kidney cells (HEK293 cells), or Caco-2 cells. Nanogel of M. pruriens seed extract prepared by ionic gelation had the hydrodynamic diameter, polydispersity index and zeta potential value of 384.53 ± 11.24 nm, 0.38 ± 0.05, and −11.23 ± 1.15 mV, respectively. The transepithelial transport of L-dopa in M. pruriens seed-extract nanogel through Caco-2 cells was measured. Nanogel containing M. pruriens seed extract at the concentration of 10 ng/mL exhibited neuroprotective activity. A jelly formulation containing M. pruriens seed-extract nanogel was successfully developed. The prepared jelly exhibited the acceptable physical and microbiological stabilities upon 6 months of the stability test. The half-life of natural L-dopa in jelly were 3.2, 0.9, and 0.6 years for storage conditions at 4, 30, and 40 °C, respectively, indicating the thermal degradation of natural L-dopa. The prepared jelly containing natural L-dopa from M. pruriens seed extract with the prominent antioxidant activity is a promising option for elderly patients suffering from Parkinson’s disease.

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“…The four peaks consist of OH stretch (3300-3200 cm -1 ), Aromatic C-H sp 2 bend (3100-3000 cm -1 ), C=O stretch (1700-1600 cm -1 ), and Alkoxy C-O stretch (1100-1000 cm -1 ) (Beauchamp, 2017). A particular compound that have abundant antioxidant activity in GTE is flavonoid monomers which is known as catechins in the form of epigallocatechin-3-gallate and epicatechin-3-gallate compounds (Theansungnoen et al, 2022). The catechin compound is a polyphenol that contains an OH-group, C-H sp 2 aromatic group, carbonyl group (C=O), an alkoxy group (C-O).…”

Section: The Chemical Properties Of Edible Film Fortified With Gtementioning

confidence: 99%

Edible Film Modification Based-on Mucuna Pruriens with Crosslink Method Incorporated with Gelatin, Sodium Alginate, and Green Tea Extract

Handayani

Soewito²,

F³

et al. 2023

Reaktor

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Health and environmental problems related to plastic food packaging are general problems. Edible film as food packaging from organic and edible materials can be a solution by increasing its structure and content. Modification of edible film from the protein of Velvet bean (Mucuna pruriens) with gelatin and alginate to modify the structure of the film and the addition of green tea leaf extract as an antioxidant. Edible films are made by combining constituent materials with the addition of a crosslink method. The addition of green tea leaf antioxidant concentration on nutritional properties, chemical properties, and shelf life of edible films through the DPPH method and antimicrobial tests showed good changes. The addition of gelatin, alginate, and green tea leaves to the mechanical and morphological properties of the film showed good indications. The effect before and after the addition of green tea leaves on the chemical structure of the edible film (OH‑ group) using FTIR analysis shows indications through changes in spectra. Modified velvet bean film with gelatin, alginate, and green tea leaves can potentially be applied in the food industry as food packaging.

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Phytochemical Analysis and Antioxidant, Antimicrobial, and Antiaging Activities of Ethanolic Seed Extracts of Four Mucuna Species

Cited by 20 publications

References 61 publications

Anti-Inflammatory Activity of Velvet Bean (Mucuna pruriens) Substances in LPS−Stimulated RAW 264.7 Macrophages

Anti-Inflammatory Activity of Velvet Bean (Mucuna pruriens) Substances in LPS−Stimulated RAW 264.7 Macrophages

Development of Jelly Loaded with Nanogel Containing Natural L-Dopa from Mucuna pruriens Seed Extract for Neuroprotection in Parkinson’s Disease

Edible Film Modification Based-on Mucuna Pruriens with Crosslink Method Incorporated with Gelatin, Sodium Alginate, and Green Tea Extract

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