Physiology of the Enterococci as Related to Their Taxonomy

Deibel, R. H.; Lake, Donald E.; Niven, C. F.

doi:10.1128/jb.86.6.1275-1282.1963

Cited by 78 publications

(38 citation statements)

References 24 publications

(20 reference statements)

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“…Some of the S.faecalis and S. faecium strains were atypical in arabinose and melezitose fermentation. Other workers (2) have reported that the fermentation reactions of these two sugars are not always appropriate for the differentiation of S. faecalis from S. faecium.…”

Section: Identification Of Fecal Streptococcimentioning

confidence: 92%

“…The broth was sterilized filtration and dispensed in 3-ml amounts into test tubes (13 by 100 mm). The methods of growth in 0.1 % methylene blue milk (8), reduction of 2,3,5-triphenyltetrazoliumchloride (TTC) (34), hydrolysis of starch and gelatin (6), polysaccharide (levan or dextran) production from 5% sucrose (6), growth in 0.04% tellurite (34), NHs production from arginine (2), and hydrolysis of hippurate (9) have been described previously.…”

Section: Methodsmentioning

confidence: 99%

“…One hundred milliliters of a 10% solution of each carbohydrate and 1 ml of indicator solution (1.6 g of bromcresol purple in 100 ml of95% ethanol) were added to 900 ml of the broth (6). Energy utilization tests were carried out according to the methods described by Deibel et al (2).…”

Section: Methodsmentioning

confidence: 99%

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Studies on Streptococci: I. Distribution of Fecal Streptococci in Man

Watanabe¹,

Shimohashi²,

Kawai³

et al. 1981

Microbiology and Immunology

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To understand the significance to the host of streptococci as part of the intestinal microflora, we first tried to investigate the distribution of human fecal streptococci on the species level. Of the selective media compared, KMN agar was more effective than the other media for the isolation of streptococci from human feces. We made an effort to improve streptococcal classification. Especially we used utilization of 1% pyruvate, 1% arginine, and 1% citrate for differentiation between Streptococcus faecalis and S. faecium. In a tellurite tolerance test, S. faecalis was distinguished more clearly from S. faecium in the medium containing 0.16 or 0.32% tellurite. We devised methods of presumptive identification of fecal streptococci from the results of the characteristics of 1,442 isolates. These methods enabled us to identify many strains rapidly. Different results in the distribution of species of streptococci between children and adults were observed. S. faecalis and S. faecium were isolated constantly from both groups. S. bovis and S. avium were isolated frequently from the feces of children. On the other hand, "viridans" streptococci, e.g. S. salivarius, S. mitis and S. MG-intermedius were present at a high frequency in, and no S. avium could be isolated from, the feces of adults.

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Section: Identification Of Fecal Streptococcimentioning

confidence: 92%

Section: Methodsmentioning

confidence: 99%

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Studies on Streptococci: I. Distribution of Fecal Streptococci in Man

Watanabe¹,

Shimohashi²,

Kawai³

et al. 1981

Microbiology and Immunology

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“…The tests used were selected from those outlined by Smith & Shattock (1962); Deibel, Lake & Niven (1963); Deibel (1964) ;Cowan & Steel (1965); Whittenbury (1965); Sharpe, Fryer & Smith (1966) ;Jones, Sackin & Sneath (1972), and Buchanan & Gibbons (1974). Inocula were prepared from active cultures (24 h old) in yeast-glucose broth (YGB) of the following composition (g/lOO ml): nutrient broth granules (Oxoid no.…”

Section: Examination Of Streptococcimentioning

confidence: 99%

The Application of Miniaturized Methods for the Characterization of Various Organisms Isolated from the Animal Gut

Jayne‐Williams

1976

Journal of Applied Bacteriology

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“…The metabolic and nutritional characteristics of the group D streptococci have been widely studied and reviewed by Deibel (1964). The nutrition of Strep.…”

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confidence: 99%

A Selective Medium for the Enumeration of Streptococcus bovis by Membrane Filtration

Oragui

Mara

1981

Journal of Applied Bacteriology

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A new selective medium (rnembrane-bovis agar) for the detection and enumeration of Streptococcus bovis is described. It has been successfully used to quantify this organism in polluted waters, sewage and faeces of humans and farm animals. This medium is based on the ability of Strep. bovis to utilize ammonium sulphate as its sole source of nitrogen. Streptococcus.fueculis, Strep. fuecium. Strep. eyuinus, Strep. sulivurius, Strep. mitis and other bacteria commonly found in water, sewage and faeces are completely inhibited.Streptococcus bovis appear to be the predominant faecal streptococci in the faeces of farm animals and absent in the faeces of humans. A total of 541 characteristic colonies (on m-BA), isolated from various sources were identified to species level. Over 97% proved to be Strep. bouis. Therefore, routine confirmatory tests on colonies growing on this medium would appear to be unnecessary. Of this proportion, 9% were non-starch hydrolysing strains of Streptococcus bovis. 9 z m B 0 the rumen. Journal of General Microbiology 18, 273-284. discharge and receiving waters.

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Physiology of the Enterococci as Related to Their Taxonomy

Cited by 78 publications

References 24 publications

Studies on Streptococci: I. Distribution of Fecal Streptococci in Man

Studies on Streptococci: I. Distribution of Fecal Streptococci in Man

The Application of Miniaturized Methods for the Characterization of Various Organisms Isolated from the Animal Gut

A Selective Medium for the Enumeration of Streptococcus bovis by Membrane Filtration

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