Physiology and metabolism of tissue-engineered skeletal muscle

Cheng, Cindy; Davis, Brittany N.; Madden, Lauran; Bursac, Nenad; Truskey, George A.

doi:10.1177/1535370214538589

Cited by 48 publications

(60 citation statements)

References 124 publications

(236 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Exogenous biophysical and biochemical stimulations are traditionally applied to mimic natural environmental cues and encourage further cellular growth and have been reviewed in detail elsewhere [71, 72]. Mechanical stimulation, in the form of progressive or cyclic stretch to mimic growth [73] and exercise [74, 75] respectively, has been proven to enhance alignment [73, 75], fusion [73, 75], myofiber hypertrophy [62], and force generation [74, 75] in engineered constructs.…”

Section: Engineering Functional Muscle Constructsmentioning

confidence: 99%

Design, evaluation, and application of engineered skeletal muscle

Juhas

Bursac

2016

Methods

Self Cite

View full text Add to dashboard Cite

For over two decades, research groups have been developing methods to engineer three-dimensional skeletal muscle tissues. These tissues hold great promise for use in disease modeling and pre-clinical drug development, and have potential to serve as therapeutic grafts for functional muscle repair. Recent advances in the field have resulted in the engineering of regenerative muscle constructs capable of survival, vascularization, and functional maturation in vivo as well as the first-time creation of functional human engineered muscles for screening of therapeutics in vitro. In this review, we will discuss the methodologies that have progressed work in the muscle tissue engineering field to its current state. The emphasis will be placed on the existing procedures to generate myogenic cell sources and form highly functional muscle tissues in vitro, techniques to monitor and evaluate muscle maturation and performance in vitro and in vivo, and surgical strategies to both create diseased environments and ensure implant survival and rapid integration into the host. Finally, we will suggest the most promising methodologies that will enable continued progress in the field.

show abstract

Section: Engineering Functional Muscle Constructsmentioning

confidence: 99%

Design, evaluation, and application of engineered skeletal muscle

Juhas

Bursac

2016

Methods

Self Cite

View full text Add to dashboard Cite

show abstract

“…When stimulated electrically or chemically, the engineered skeletal muscle responded much like a normal tissue in terms of releasing calcium ions followed by twitching. The average tetanus force generated by the engineered muscles was far lower than that reported for adult muscle, but was similar to the values measured in foetal human muscle [154,155]. Testing three classes of pharmaceutical drugs revealed that they had similar effects on the engineered muscle as on normal muscle tissue under clinical settings.…”

Section: Drug Screeningmentioning

confidence: 79%

Hydrogel biomaterials and their therapeutic potential for muscle injuries and muscular dystrophies

Lev¹,

Seliktar²

2018

J. R. Soc. Interface.

View full text Add to dashboard Cite

Muscular diseases such as muscular dystrophies and muscle injuries constitute a large group of ailments that manifest as muscle weakness, atrophy or fibrosis. Although cell therapy is a promising treatment option, the delivery and retention of cells in the muscle is difficult and prevents sustained regeneration needed for adequate functional improvements. Various types of biomaterials with different physical and chemical properties have been developed to improve the delivery of cells and/or growth factors for treating muscle injuries. Hydrogels are a family of materials with distinct advantages for use as cell delivery systems in muscle injuries and ailments, including their mild processing conditions, their similarities to natural tissue extracellular matrix, and their ability to be delivered with less invasive approaches. Moreover, hydrogels can be made to completely degrade in the body, leaving behind their biological payload in a process that can enhance the therapeutic process. For these reasons, hydrogels have shown great potential as cell delivery matrices. This paper reviews a few of the hydrogel systems currently being applied together with cell therapy and/or growth factor delivery to promote the therapeutic repair of muscle injuries and muscle wasting diseases such as muscular dystrophies.

show abstract

“…The previous MPS thematic issue in EBM published manuscripts highlighting early progress from both of these efforts. [26][27][28][29][30][31][32][33][34][35][36][37][38][39][40][41][42] It was recognized that MPS might help close the hermeneutic circle of biology, which describes how it is impossible to understand the whole (the compete organism) without understanding the parts (the genome, proteome, metabolome, etc. ), and that understanding the parts requires an understanding of the whole.…”

Section: The Entry Of Mps Onto the Biomedical Scenementioning

confidence: 99%

“…A much more comprehensive battery of techniques is already in regular use in the pharmaceutical industry, including genomics, transcriptomics, and proteomics. In recent MPS studies, we are seeing functional measurements such as contractility, calcium signaling, and electrophysiological responses of cardiac and skeletal muscle, 37,[69][70][71][72][73][74][75] electrical resistance and molecular permeability of barriers, [76][77][78][79][80][81][82] and metabolomic responses to inflammatory challenges. 78 In this issue, the Parker group uses a variety of measures, including gene expression profiling, to quantify factors that affect maturation of neonatal rat cardiomyocytes into a more mature phenotype.…”

Section: Fitting Into the "Grand Scheme"mentioning

confidence: 99%

Fitting tissue chips and microphysiological systems into the grand scheme of medicine, biology, pharmacology, and toxicology

Watson

Hunziker

Wikswo

2017

Exp Biol Med (Maywood)

View full text Add to dashboard Cite

Impact statementMicrophysiological systems (MPS), which include engineered organoids and both individual and coupled organs-on-chips and tissue chips, are a rapidly growing topic of research that addresses the known limitations of conventional cellular monoculture on flat plastic -a wellperfected set of techniques that produces reliable, statistically significant results that may not adequately represent human biology and disease. As reviewed in this article and the others in this thematic issue, MPS research has made notable progress in the past three years in both cell sourcing and characterization. As the field matures, currently identified challenges are being addressed, and new ones are being recognized. Building upon investments by the Defense Advanced Research Projects Agency, National Institutes of Health, Food and Drug Administration, Defense Threat Reduction Agency, and Environmental Protection Agency of more than $200 million since 2012 and sizable corporate spending, academic and commercial players in the MPS community are demonstrating their ability to meet the translational challenges required to apply MPS technologies to accelerate drug development and advance toxicology. AbstractMicrophysiological systems (MPS), which include engineered organoids (EOs), single organ/tissue chips (TCs), and multiple organs interconnected to create miniature in vitro models of human physiological systems, are rapidly becoming effective tools for drug development and the mechanistic understanding of tissue physiology and pathophysiology. The second MPS thematic issue of Experimental Biology and Medicine comprises 15 articles by scientists and engineers from the National Institutes of Health, the IQ Consortium, the Food and Drug Administration, and Environmental Protection Agency, an MPS company, and academia. Topics include the progress, challenges, and future of organs-on-chips, dissemination of TCs into Pharma, children's health protection, liver zonation, liver chips and their coupling to interconnected systems, gastrointestinal MPS, maturation of immature cardiomyocytes in a heart-on-a-chip, coculture of multiple cell types in a human skin construct, use of synthetic hydrogels to create EOs that form neural tissue models, the blood-brain barrier-on-a-chip, MPS models of coupled female reproductive organs, coupling MPS devices to create a body-on-a-chip, and the use of a microformulator to recapitulate endocrine circadian rhythms. While MPS hardware has been relatively stable since the last MPS thematic issue, there have been significant advances in cell sourcing, with increased reliance on human-induced pluripotent stem cells, and in characterization of the genetic and functional cell state in MPS bioreactors. There is growing appreciation of the need to minimize perfusate-to-cell-volume ratios and respect physiological scaling of coupled TCs. Questions asked by drug developers are followed by an analysis of the potential value, costs, and needs of Pharma. Of highest value and lowest switching costs may be the d...

show abstract

Physiology and metabolism of tissue-engineered skeletal muscle

Cited by 48 publications

References 124 publications

Design, evaluation, and application of engineered skeletal muscle

Design, evaluation, and application of engineered skeletal muscle

Hydrogel biomaterials and their therapeutic potential for muscle injuries and muscular dystrophies

Fitting tissue chips and microphysiological systems into the grand scheme of medicine, biology, pharmacology, and toxicology

Contact Info

Product

Resources

About