Physiological studies on supercooled killifish (<i>Fundulus heteroclitus</i>). IV. Carbohydrate metabolism in hypophysectomized killifish at subzero temperatures

Umminger, Bruce L.

doi:10.1002/jez.1401810208

Cited by 9 publications

(2 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…F. heteroclitus has been shown to survive for extended periods in temperatures ranging from -1.5°C (Umminger 1972) to 36°C (Garside & Chin-Yuen-Kee 1972). C. variegatus has been found in waters with salinities ranging from 0 to 142 ppt (Simpson & Gunter 1956, Nordlie 1985, Dunson et al 1998) and F. heteroclitus from 0 to 120 ppt (Griffith 1974).…”

Section: Summer Structuring Mechanismsmentioning

confidence: 99%

Seasonally varying importance of abiotic and biotic factors in marsh-pond fish communities

Layman¹,

Smith²,

Herod³

2000

Mar. Ecol. Prog. Ser.

View full text Add to dashboard Cite

A series of field and laboratory experiments examined the factors that control fishassemblage structure in barrier islands ponds on the Eastern Shore of Virginia. We investigated why surf-zone fishes, which are introduced to the island ponds during periodic wash-over events, are unable to persist for extended periods. We simulated over-wash events by collecting fishes from the shallow surf-zone and introducing them into 0.56 m 2 field enclosures. During summer months, surfzone fishes typically died within 24 h. Levels of dissolved oxygen and temperature frequently exceeded the tolerance limits of the surf-zone fishes (demonstrated in a series of laboratory experiments), suggesting that these variables were responsible for fish mortality. In non-summer months, when dissolved oxygen and temperature stresses were ameliorated, surf-zone fishes could survive for months in the ponds. We conducted field-enclosure experiments at 3 fish densities in order to evaluate the survival and condition of a common surf-zone fish, Membras martinica (the rough silverside):

show abstract

Section: Summer Structuring Mechanismsmentioning

confidence: 99%

Seasonally varying importance of abiotic and biotic factors in marsh-pond fish communities

Layman¹,

Smith²,

Herod³

2000

Mar. Ecol. Prog. Ser.

View full text Add to dashboard Cite

show abstract

“…This slightly larger difference in temperature (5°C) between the upper portion of ditches and the upper portion of creeks could be a contributing factor to the observed nekton differences between ditches and creeks as a whole in less tolerant marsh species. However, killifish (and perhaps other marsh residents) are known to tolerate a wide range of both temperature and salinity, especially Fundulus heteroclitus (Garside & Chin-Yuen-Kee 1972, Umminger 1972, Griffith 1974.…”

Section: Within-ditch and Within-creek Nekton Patternsmentioning

confidence: 99%

Comparison of salt marsh creeks and ditches as habitat for nekton

Corman

Roman²

2011

Mar. Ecol. Prog. Ser.

View full text Add to dashboard Cite

Salt marshes are dynamic systems supporting a diverse assemblage of resident and transient nekton (free-swimming fish and decapod crustaceans). Within a marsh, many sub-habitats are used by nekton, including ditches and natural creeks. While the use of natural creeks is well documented, the role of ditches as habitat for nekton remains less well known. The present study describes the nekton-support function of this prolific marsh sub-habitat, and compares it to tidal creeks. Sampling was conducted in the summer of 2008 in a microtidal, polyhaline salt marsh on Fire Island, NY, USA. Ditches and creeks were found to have different nekton communities. Creeks had significantly higher species richness and represented nearly all ditch species. Prolonged anoxia (up to 18 h) in ditches is suggested as a significant factor contributing to differences in the creek and ditch nekton community. Upper portions of ditches and creeks differed for resident nekton species, with higher species richness and density of the mummichog Fundulus heteroclitus in the upper portion of creeks than in the upper portion of ditches. Additional differences were seen in the nekton community composition of open and naturally plugged ditches, where the densities of individual species (such as F. heteroclitus, which was more abundant in open ditches) also differed. Ditches are a major habitat type in salt marshes of the northeastern USA, such as at the Fire Island marsh site studied here, and as efforts are considered to remove ditches from marshes as a habitat restoration technique, their nekton support function should be considered.

show abstract

Role of islet tissue in the cold‐induced hyperglycemia of the killifish, Fundulus heteroclitus

Umminger

Bair

1973

J. Exp. Zool.

View full text Add to dashboard Cite

Islet tissue histology and serum glucose concentrations were examined in salt-water-adapted killifish, Fundulus heteroclitus, acclimated to 20°C and to -1.5"C for one and ten days. Exposure to subzero temperatures produced a hyperglycemia accompanied by hypertrophy and degranulation of alpha cells in the islets and by atrophy of the islet beta cells with no changes in granulation. The cold-induced hyperglycemia presumably resulted from an increase in circulating levels of glucagon and from a failure of the beta cells to release insulin.Exposure to subzero temperatures elicits a marked hyperglycemia in the killifish, Fundulus heteroclitus, which persists for several weeks (Umminger, '69, '70). This cold-induced hyperglycemia results from a breakdown of hepatic glycogen (Umminger, '70) produced by a n increase in the specific activity of hepatic glycogen phosphorylase (Benziger and Umminger, '72). Previous studies have demonstrated that the hormonal regulation of this hyperglycemic response is not under pituitary control and the suggestion has been made that the pancreatic islets are probably involved (Umminger, '71, '72). The present investigation provides histological evidence that the islets of the killifish are indeed implicated in the elicitation of the cold-induced hyperglyceniia. MATERIALS AND METHODSAdult male F. heteroclitus were obtained by air freight from the Supply Department of the Marine Biological Laboratory in Woods Hole, Massachusetts, on October 27, 1970. The fish were subsequently maintained under a regime of 12 hours of light per day i n Instant Ocean aquaria (salinity 33"/00) at the University of Cincinnati. The killifish were fed daily ad libitum with Rangen's Quality Trout Feed, but were not fed the day of autopsy. One group of five fish was kept at 20°C for 52 weeks. A second group of five fish was maintained at 10°C for 28 weeks and at 5°C for 24 weeks; at the end of this acclimation period, the fish were transferred to a cold room held at -1.5"C and the water at 5°C was allowed to cool gradually to -1.5"C in six hours; the fish remained at -1.5" C for one day before autopsy. A third group of three fish was maintained at 10°C for 28 weeks, at 5°C for 24 weeks and at -1.5"C for ten days.At autopsy, the tails of fish lightly anesthetized with tricaine methanesulfonate (MS 222) were severed and the free-flowing blood was collected from the caudal artery in microhematocrit tubes. The blood was allowed to clot before centrifugation and the serum from individual fish was stored in tightly corked vials at -20°C. The following day the samples were briefly thawed for withdrawal of microliter samples of serum for the chemical deterniination of glucose. Serum glucose was determined using a n ultramicro adaptation (Pickford et al., '69) of the Glucostat (Worthington Biochemic a1 Corp .) enzymatic method, following the procedure of Saifer and Gerstenfeld ('58).The large principal islets (Brockmann bodies) at the base of the gallbladder near the cystic duct were fixed i n Bouin's fluid at the t...

show abstract

Physiological studies on supercooled killifish (Fundulus heteroclitus). IV. Carbohydrate metabolism in hypophysectomized killifish at subzero temperatures

Cited by 9 publications

References 13 publications

Seasonally varying importance of abiotic and biotic factors in marsh-pond fish communities

Seasonally varying importance of abiotic and biotic factors in marsh-pond fish communities

Comparison of salt marsh creeks and ditches as habitat for nekton

Role of islet tissue in the cold‐induced hyperglycemia of the killifish, Fundulus heteroclitus

Contact Info

Product

Resources

About