Donald A. MacKenzie, Laurent C.G. Gendron, David J. Jeenes, David B. Archer
Abstract:Physiological factors affecting hen eggwhite lysozyme and native glucoamylase production by Aspergillus niger have been examined in batch culture. Expression of the genes encoding both proteins was controlled by the glucoamylase promoter. In standard expression medium (ACMS/N/P), secreted lysozyme yields were found to be maximal at 20-25 degrees C (8-10 mg l-1) and markedly reduced at 30-37 degrees C (3-5 mg l-1). Production of lysozyme exhibited similar induction or repression profiles to that of endogenous g…
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