Physiological, histopathological and cellular immune effects of Pergularia tomentosa extract on Locusta migratoria nymphs

Miladi, Meriam; Abdellaoui, Khemais; Hamouda, Amel Ben; Boughattas, Iteb; Mhafdhi, Mouna; Acheuk, Fatma; Halima-Kamel, Monia Ben

doi:10.1016/s2095-3119(19)62704-8

Cited by 10 publications

(9 citation statements)

References 37 publications

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“…All of the tested compounds showed AchE inhibition. According to Abdellaoui et al (2019), the AchE activity in the 5 th instar nymphs of the migratory locust Locusta migratoria was reduced after treatment with methanolic extract of olive leaves. Treatment of newly moulted 4 th and 5 th instar nymphs of L. migratoria with methanolic extract of Pergularia tomentosa resulted in a reduction of the activity of AchE (Miladi et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

“…According to Abdellaoui et al (2019), the AchE activity in the 5 th instar nymphs of the migratory locust Locusta migratoria was reduced after treatment with methanolic extract of olive leaves. Treatment of newly moulted 4 th and 5 th instar nymphs of L. migratoria with methanolic extract of Pergularia tomentosa resulted in a reduction of the activity of AchE (Miladi et al, 2019). AchE activity in adults of Sitophilus oryzae and Tribolium castaneum was inhibited after treatment with the Mentha piperita essential oil (Rajkumar et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

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Disturbance of the Acetylcholinesterase Activity in Haemolymph and Fat Bodies of The Desert Locust, Schistocerca gregaria (Orthoptera: Acrididae) by Margosan-O® (0.3% Azadirachtin)

Basiouny

2021

Egyptian Academic Journal of Biological Sciences. A, Entomology

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Disturbance of the Acetylcholinesterase Activity in Haemolymph and Fat Bodies of The Desert Locust, Schistocerca gregaria (Orthoptera: Acrididae) by Margosan-O® (0.3% Azadirachtin)

Basiouny

2021

Egyptian Academic Journal of Biological Sciences. A, Entomology

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“…The ethanolic solution (20 μL) was applied to a freshly emerged adult female (0 to 12 h post emergence) on their dorsum with a microapplicator at concentrations of 0.24% and 0.96% designed respectively AFPt C1 and AFPt C2. Concentrations were chosen in accordance with amounts used in previous work (Miladi et al ., 2019) when testing the effects of P. tomentosa crude extract against Locusta migratoria (Acrididae) nymphs. Four days later, the locusts were treated again with the same solution.…”

Section: Methodsmentioning

confidence: 99%

Effects of Pergularia tomentosa active fraction on the oogenesis of the desert locust, Schistocerca gregaria: ovarian biochemical composition, and effects on the ecdysteroid pathway

et al. 2020

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Fractionation of the methanolic extract of Pergularia tomentosa, using column chromatography, resulted in the separation of 10 fractions and only the active one (that gave mortality >70%) was kept and denoted the active fraction (AFPt). Adult female Schistocerca gregaria were treated with two concentrations of AFPt, 0.24% and 0.96%, to investigate the effect on the female reproductive potential. Both concentrations of AFPt significantly increased the duration between adult emergence and first oviposition, as well as affected fecundity and fertility. Furthermore, the effects of AFPt on vitellogenesis and ecdysteroid titres were examined. Biochemical analyses revealed a significant decrease in protein, lipid and carbohydrate contents in ovaries. This study also showed that AFPt induced a significant decrease in the ovarian ecdysteroids levels, as well as the haemolymph ecdysteroid titres. The transcript levels of the ecdysteroid synthesis enzymes Spook and Shadow were significantly decreased when treated with the highest concentration of AFPt, while transcript levels of the ecdysteroid synthesis enzymes Phantom and Disembodied increased significantly in animals treated with AFPt. Treatment with AFPt also resulted in a significantly higher transcript level of both the ecdysone receptor (EcR) and its partner ultraspiracle/retinoid‐X‐receptor (USP/RXR).

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“…It was also found that the PME extract induced cellular immune reactions by affecting the number and morphology of differential haemocytes. (Miladi et al, 2019). The phytochemical constituents of P.tomentosa plants grown in the district of Hail and other geographical regions.…”

Section: Evaluation Of Cytotoxic Effects Of Methanolic Extract Of Permentioning

confidence: 99%

Evaluation of Cytotoxic Effects of Methanolic Extract of Pergularia tomentosa L Growing Wild in KSA

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Alshammari

2021

Asian Pac J Cancer Prev

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Background: Pergularia tomentosa is a member of the Apocynaceae family found in a wide geographical region including the Gulf region, North Africa and the Middle East. It is known as Fattaka, Ghalqa or Am Lebina in Saudi Arabia, It is used as a remedy for the treatment of skin sores, asthma, and bronchitis. Objective: This study aims to investigate the cytotoxic effects of methanolic extract and Latex (milky secretion) extract. Methods: The stem of Pergularia tomentosa was cut, air dried and soaked for 72 h with methanol repeatedly three times. The crude latex (milk extract) was collected from healthy stem parts of P. tomentosa L by cutting the petiole of leaves, and left to flow where a thick white liquid (Milky) were secreted, collected in amber glass tube and extracted with methanol. Further, the methanolic extract was fractionated by subsequent extraction with various solvents, viz. n-hexane, ethyl acetate and methanol. The cytotoxic effects of Pergularia tomentosa L were evaluated using three cancer cell lines of colon carcinoma (HCT-116), hepatocellular carcinoma (HepG2) and breast carcinoma (MCF-7). The cytotoxic effects of Pergularia tomentosa L extracts against HCT-116, HepG2, and MCF-7 were determined by crystal violet staining method. Results: The potency of plant extract to decrease the cell viability of human cancer cells was arranged in descending order as follows: Methanol extract (IC50 = 10.2 μg/ml, 13.6 μg/ml and 29.5μg/ml, respectively). > Milky secretion extract (IC50 = 52.6 μg/ml, 58.6 μg/ml and 120 µg/ml, respectively). Methanolic extract was strong cytotoxic activity against HCT-116 and HepG2 (IC50= 10.2, 13.6 µg/ml, respectively) and moderately activity against MCF-7 (IC50= 29.5 µg/ml). The Milky extract exhibited moderate activity against HCT-116 and HepG2 (IC50= 52.6-58.6 µg/ ml, respectively) and weak activity against MCF-7 (120.0 µg/ml). Conclusion: The methanol extract of Pergularia tomentosa L showed higher cytotoxic effect as compared to the Latex (Milky secretion) extract. These extracts can be used as natural antitumor. In Future modern chromatographic separations are needed to get more quantity of metabolites. Further detailed investigation of the isolated metabolites is required to identify the phytoconstituents responsible for antioxidant and cytotoxic effects.

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Physiological, histopathological and cellular immune effects of Pergularia tomentosa extract on Locusta migratoria nymphs

Cited by 10 publications

References 37 publications

Disturbance of the Acetylcholinesterase Activity in Haemolymph and Fat Bodies of The Desert Locust, Schistocerca gregaria (Orthoptera: Acrididae) by Margosan-O® (0.3% Azadirachtin)

Disturbance of the Acetylcholinesterase Activity in Haemolymph and Fat Bodies of The Desert Locust, Schistocerca gregaria (Orthoptera: Acrididae) by Margosan-O® (0.3% Azadirachtin)

Effects of Pergularia tomentosa active fraction on the oogenesis of the desert locust, Schistocerca gregaria: ovarian biochemical composition, and effects on the ecdysteroid pathway

Evaluation of Cytotoxic Effects of Methanolic Extract of Pergularia tomentosa L Growing Wild in KSA

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