Physiological, biochemical, and fluorescence parameters of senescing sugar beet leaves in the vegetative phase of growth

Romanova, A. K.; Semenova, Galina; Novichkova, N. S.; Ignat’ev, A. R.; Mudrik, V. A.; Иванов, Б. Н.

doi:10.1134/s1021443711020178

Cited by 11 publications

(14 citation statements)

References 15 publications

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“…Plastids of T cells were not as aberrant in ultrastructure as those of HNO cells, but these were smaller than N plastids and did not contain starch grains. Starch grains were observed in sugar beet leaf chloroplasts, and the regulation of starch size in the chloroplast could be influenced by the cytokinin BA (Paramonova et al 2002;Romanova et al 2011). When ultrathin sections are cut for electron microscopy, starch grains occurring as robust inclusions in the stroma of plastids can be pushed out of the section, leaving a void.…”

Section: Discussionmentioning

confidence: 99%

Morphological and proteomic analyses of sugar beet cultures and identifying putative markers for cell differentiation

Pavoković

Poljuha²,

Horvatić

et al. 2011

Plant Cell Tiss Organ Cult

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Normal (N), habituated nonorganogenic (HNO), and tumour (T) sugar beet cell lines were analysed in order to establish specific patterns of extracellular proteins and identify protein markers that might explain the distinct phenotypical characteristics. Electron microscopy showed that the ultrastructure of N cells corresponds to that of parenchyma cells, and that these cells contain plastids with large starch grains. HNO and T cells had enlarged, lobed nuclei with an increased number of nucleoli; the number of nuclei in HNO cells was greater than in T cells. The T plastids were elongated, with reduced thylakoids and abundant phytoferritin deposits, while HNO plastids were small and vacuolated, with an irregular, underdeveloped thylakoid system. The extracellular proteome of the cells was separated by sodium dodecyl sulphate polyacrylamide gel electrophoresis. Greater differences in protein expression were observed between the HNO and N lines than between the T and N lines. Sixteen of the most prominent bands differentially expressed among the cell lines were cut out from the gel and analyzed by mass spectrometry. Cell wall-modifying enzymes were identified, including a peroxidase whose expression was twofold higher in N and T tissue than in HNO tissue; pectinesterase, which was expressed at a level threefold lower in the T line than in the other cell lines; and xyloglucan endotransglucosylase, which was expressed at a level sixfold higher in HNO and T tissue. Three proteins belonged to the chitinase gene family and their expression was higher in HNO and T tissue than in N tissue. The differential expression of these proteins suggests that these play a role in cell line-specific cell wall composition and cell-to-cell adhesion.

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Section: Discussionmentioning

confidence: 99%

Morphological and proteomic analyses of sugar beet cultures and identifying putative markers for cell differentiation

Pavoković

Poljuha²,

Horvatić

et al. 2011

Plant Cell Tiss Organ Cult

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“…1 и 2). Лист среднего яруса отличался высоким уровнем хлорофилла, растворимых белков и Рубиско, что указывало на высокую активность в нем метаболических процессов, а нижний лист -пониженным уровнем хлорофилла, растворимых белков и Рубиско, что является индикатором процессов старения, которые начались в этом органе [Romanova et al, 2011).…”

Section: Discussionunclassified

“…Возможное участие ауксинов также представляло интерес в связи с их ролью в регуляции роста листьев [Веселов и др., 2008]. Наряду с содержанием сахаров мы также оценили содержание хлорофилла и белков, поскольку полученные ранее данные свидетельствовали об информативности этих характеристик как показателей созревания и старения листьев и усиления их донорных функций [Romanova et al, 2011].…”

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Hormone Content and Abscisic Acid Localization in Leaves of Different Age of Sugar Beet

Romanova¹,

Novichkova²,

Vysotskaya³

et al. 2018

ÈKOBIOTEH

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“…It is known that these parameters are affected by stress factors [3][4][5][6] and undergo changes during natural senescence of plant leaves [5,[7][8][9][10][11][12]. The age dependence of chlorophyll fluorescence parameters is a well established fact.…”

Section: Introductionmentioning

confidence: 99%

“…The age dependence of chlorophyll fluorescence parameters is a well established fact. However, the extent of this dependence for individual parameters remains poorly examined; in many cases researchers were satisfied by simple comparison of fluorescence characteristics in mature and senescent leaves using rough estimates of leaf age [7][8][9][10][11]13]. We showed ear lier that the ratio F v /F m , characterizing the maximal quantum efficiency of PSII photochemistry, under goes only slight (~8%) age dependent changes under normal growth conditions [12,14], whereas the ratio F p /F t (F p and F t are fluorescence intensities at the peak level P and under steady state, respectively [1]) exhib its age dependent changes with an amplitude of at least 40% [14].…”

Section: Introductionmentioning

confidence: 99%

Chlorophyll fluorescence as an indicator of age-dependent changes in photosynthetic apparatus of wheat leaves

Nesterenko

Shikhov

Tikhomirov

2015

Russ J Plant Physiol

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Wheat (Triticum sativus L.) seedlings of various ages (2 to 16 day old plants) were used to study age dependent changes in the chlorophyll fluorescence induction (CFI) at various light intensities during flu orescence measurements. Plants were raised in a growth chamber using hydroponics with expanded clay, con trolled environmental conditions, and 690 µmol/(m 2 s) photon flux density (PFD) of photosynthetically active radiation (PAR). Parameters of CFI were determined under actinic PFD of 380, 580, 820, and 1340 µmol/(m 2 s) PAR. The fifth leaf from the stem base, exposed to uniform lighting, was sampled for mea surements. This leaf emerged at the plant age of 16 days. Based on fluorescence data, we calculated the max imal photochemical quantum yield of photosystem II (F v /F m ), the effective photochemical quantum yield of PSII (Yield), parameters of photochemical (qP) and non photochemical (qN and NPQ) quenching of chlo rophyll fluorescence, the F p /F t ratio, and the "vitality index" (fluorescence decrease ratio, R fd ). At moderate actinic PFD, applied commonly in PAM fluorometers (about 380 µmol/(m 2 s)), age dependent changes in NPQ, F p /F t , and R fd were observed. Analysis of CFI parameters in wheat leaves of different ages at PFD increasing from 380 to 820 µmol/(m 2 s) revealed that R fd , NPQ, and qN are the most sensitive markers of the leaf age among all parameters tested. These suitable indicators can be used for rapid assessment of the leaf age.

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Physiological, biochemical, and fluorescence parameters of senescing sugar beet leaves in the vegetative phase of growth

Cited by 11 publications

References 15 publications

Morphological and proteomic analyses of sugar beet cultures and identifying putative markers for cell differentiation

Morphological and proteomic analyses of sugar beet cultures and identifying putative markers for cell differentiation

Hormone Content and Abscisic Acid Localization in Leaves of Different Age of Sugar Beet

Chlorophyll fluorescence as an indicator of age-dependent changes in photosynthetic apparatus of wheat leaves

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