Physiological and biochemical analysis of barley (Hordeum vulgare) genotypes with contrasting salt tolerance

Ouertani, Rim Nefissi; Abid, Ghassen; Chikha, Mariem Ben; Boudaya, Oumaima; Mejri, Samiha; Karmous, Chahine; Ghorbel, Abdelwahed

doi:10.1007/s11738-022-03388-5

Cited by 7 publications

(3 citation statements)

References 38 publications

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“…Further, APX and CAT enzymes are involved in catabolizing hydrogen peroxides into H 2 O and O 2 in plants. Higher activities of SOD (shoot), CAT (root) and APX (root and shoot both) strengthen antioxidant potential at combined-stage salinity in CSR36 compared with that of Jaya, which coincides with earlier observation wherein increased SOD, APX and CAT activities have been shown to contribute to enhanced salt tolerance [ 32 , 33 ]. PCA analysis validated CAT and APX activities in root as the major drivers for salt tolerance in CSR36 ( Figure 7 C,E,F).…”

Section: Discussionsupporting

confidence: 88%

Antioxidant Defense and Ionic Homeostasis Govern Stage-Specific Response of Salinity Stress in Contrasting Rice Varieties

Kumar,

Srivastava,

Sharma

et al. 2024

Plants

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Salt stress is one of the most severe environmental stresses limiting the productivity of crops, including rice. However, there is a lack of information on how salt-stress sensitivity varies across different developmental stages in rice. In view of this, a comparative evaluation of contrasting rice varieties CSR36 (salt tolerant) and Jaya (salt sensitive) was conducted, wherein NaCl stress (50 mM) was independently given either at seedling (S-stage), tillering (T-stage), flowering (F-stage), seed-setting (SS-stage) or throughout plant growth, from seedling till maturity. Except for S-stage, CSR36 exhibited improved NaCl stress tolerance than Jaya, at all other tested stages. Principal component analysis (PCA) revealed that the improved NaCl stress tolerance in CSR36 coincided with enhanced activities/levels of enzymatic/non-enzymatic antioxidants (root ascorbate peroxidase for T- (2.74-fold) and S+T- (2.12-fold) stages and root catalase for F- (5.22-fold), S+T- (2.10-fold) and S+T+F- (2.61-fold) stages) and higher accumulation of osmolytes (shoot proline for F-stage (5.82-fold) and S+T+F- (2.31-fold) stage), indicating better antioxidant capacitance and osmotic adjustment, respectively. In contrast, higher shoot accumulation of Na+ (14.25-fold) and consequent increase in Na+/K+ (14.56-fold), Na+/Mg+2 (13.09-fold) and Na+/Ca+2 (8.38-fold) ratio in shoot, were identified as major variables associated with S-stage salinity in Jaya. Higher root Na+ and their associated ratio were major deriving force for other stage specific and combined stage salinity in Jaya. In addition, CSR36 exhibited higher levels of Fe3+, Mn2+ and Co3+ and lower Cl− and SO42−, suggesting its potential to discriminate essential and non-essential nutrients, which might contribute to NaCl stress tolerance. Taken together, the findings provided the framework for stage-specific salinity responses in rice, which will facilitate crop-improvement programs for specific ecological niches, including coastal regions.

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Section: Discussionsupporting

confidence: 88%

Antioxidant Defense and Ionic Homeostasis Govern Stage-Specific Response of Salinity Stress in Contrasting Rice Varieties

Kumar,

Srivastava,

Sharma

et al. 2024

Plants

View full text Add to dashboard Cite

show abstract

“…Further, APX and CAT enzymes are involved in catabolizing hydrogen peroxides into H 2 O and O 2 in plants. Higher activities of SOD (shoot), CAT (root) and APX (root and shoot both) strengthen antioxidant potential at combined-stage salinity in CSR36 compared with that of Jaya, which coincides with earlier observation wherein increased SOD, APX and CAT activities have been shown to contribute to enhanced salt tolerance [32,33]. PCA analysis validated CAT and APX activities in root as the major drivers for salt tolerance in CSR36 (Figure 7C,E,F).…”

Section: Nacl-induced Toxicity Is Developmental Stage-specificsupporting

confidence: 88%

Antioxidant Defense and Ionic Homeostasis Govern Stage-Specific Response of Salinity Stress in Contrasting Rice Varieties

Kumar,

Srivastava,

Sharma

et al. 2024

Preprint

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Salt stress is one of the most severe environmental stresses limiting the productivity of crops, including rice. However, there is a lack of information on how salt-stress sensitivity varies across different developmental stages in rice. In view of this, a comparative evaluation of contrasting rice varieties CSR36 (salt tolerant) and Jaya (salt sensitive) was conducted, wherein NaCl stress (50 mM) was independently given either at seedling (S-stage), tillering (T-stage), flowering (F-stage), seed-setting (SS-stage) or throughout the plant growth, from seedling till maturity. Except for S-stage, CSR36 exhibited improved NaCl stress tolerance than Jaya, at all other tested stages. Principal component analysis (PCA) revealed that the improved NaCl stress tolerance in CSR36 coincided with enhanced activities/levels of enzymatic/non-enzymatic antioxidants (root ascorbate peroxidase for T- and S+T stages and root catalase for F-, S+T and S+T+F-stages) and higher accumulation of osmolytes (shoot proline for F-stage and S+T+F-stage), indicating better antioxidant capacitance and osmotic adjustment, respectively. In contrast, higher shoot accumulation of Na+ and consequent increase in Na+/K+, Na+/Ca+2 and Na+/Mg+2 ratio in root and shoot, were identified as major variables associated with NaCl stress induced growth reduction in Jaya. In addition, CSR36 exhibited higher levels of Fe3+, Mn2+ and Co3+ and lower Cl- and SO42-, suggesting its potential to discriminate essential and non-essential nutrients, which might contribute to NaCl stress tolerance. Taken together, the findings provided the frame-work for stage-specific salinity responses in rice, which will facilitate crop-improvement programs for specific ecological niches including the coastal regions. Rice, salt stress, stage-specific salinity, antioxidant defense, ionic homeostasis, salt tolerance

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“…The principal limits to grain yield formation in the Mediterranean basin are in continuous increase year-to-year as a consequence of the climate change and abiotic factors (Boussakouran et al 2021). Salinity is one of the osmotic stressors that have affected plant growth and development, as shown by the reduction in biomass output and shoots expansion (Nefissi Ouertani et al 2022). In addition, under salt stress, plants demonstrate a wide range of physiological, biochemical, and molecular processes (Narimani et al 2020).…”

Section: Discussionmentioning

confidence: 99%

Biomass partitioning and physiological responses of four Moroccan barley varieties subjected to salt stress in a hydroponic system

Bouhraoua,

Ferioun,

Nassira

et al. 2023

J Plant Biotechnol

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A hydroponics experiment was performed to study the physiological and biochemical changes in Moroccan barley (Hordeum vulgare L.) varieties cultivated under salt stress conditions. Four barley varieties were grown under exposure to three salt concentrations, including 0, 200, and 300 mM NaCl. The ANOVA for both salt stress-sensitive and resistant varieties indicated that salt treatment represented the main source of variability in all studied traits. Salt treatment significantly reduced root and shoot dry weight (RDW and SDW), relative water content (RWC), and chlorophyll content (Chl a, Chl b, and Chl T). However, increases in electrolyte leakage (EL) along with proline and total soluble sugar (TSS) contents were recorded. In addition, large variations in all measured traits were found between varieties. The 'Massine' and 'Laanaceur' varieties displayed relatively higher RDW and SDW values. The 'Amira' and 'Adrar' varieties showed lower RWC values and Chl contents than those of the controls indicating their relative sensitivity to salt stress. Principal component analysis revealed that most of the variation was captured by PC1 (72% of the total variance) which grouped samples into three categories according to salt treatment. Correlation analyses highlighted significant associations between most parameters. Positive relationships were found between RDW, SDW, RWC, Chl content, and soluble proteins contents, while all of these parameters were negatively associated with EL intensity, proline content, and TSS content. The results from this study showed that the 'Massine' and 'Laanaceur' varieties were relatively salt-tolerant. These two salt-tolerant varieties present a good genetic background for breeding of barley varieties showing high salt tolerance.

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Physiological and biochemical analysis of barley (Hordeum vulgare) genotypes with contrasting salt tolerance

Cited by 7 publications

References 38 publications

Antioxidant Defense and Ionic Homeostasis Govern Stage-Specific Response of Salinity Stress in Contrasting Rice Varieties

Antioxidant Defense and Ionic Homeostasis Govern Stage-Specific Response of Salinity Stress in Contrasting Rice Varieties

Antioxidant Defense and Ionic Homeostasis Govern Stage-Specific Response of Salinity Stress in Contrasting Rice Varieties

Biomass partitioning and physiological responses of four Moroccan barley varieties subjected to salt stress in a hydroponic system

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