PHYSIOLOGIC ROLE OF COLD‐INSOLUBLE GLOBULIN IN SYSTEMIC HOST DEFENSE: IMPLICATIONS OF ITS CHARACTERIZATION AS THE OPSONIC α₂‐SURFACE‐BINDING GLYCOPROTEIN*

Abstract

UNANUE. E. R. 1972. The regulatory role of macrophages in antigenic stimulation. Adv. Immunol. 15: 95-165. SABA, T. M. & N. R. DI LUZIO. 1969. Reticuloendothelial blockade and recovery as a function of opsonic activity. Amer. J. Physiol. 216: 197-205. 2.3. 4. .

Circulating immunoreactive and bioassayable opsonic plasma fibronectin during experimental tumour growth

“…Before the immunoassay became available, it was assumed that deficiency of opsonic activity during advanced cancer represented depletion as observed after RE blockade (Blumenstock et al, 1977a;Saba & Di Luzio, 1969) or surgery and trauma (Saba et al, 1978a,b;Scovill et at., 1978). Confirmation of depletion after blockade (Blumenstock et al, 1977b) or trauma (Saba & Cho, 1979;Saba et al, 1978b) has been obtained by immunoassay, but the present data suggest an alternate mechanism during cancer. Tumour growth and spread may produce either inhibition and/ or proteolytic destruction of biologically active plasma fibronectin, resulting in immunoreactive fragments without biological activity.…”

“…An immunoabsorbent consisting of glutaraldehyde-cross-linking opsonin-deficient serum can be used for preparation of monospecific antiserum as previously documented (Blumenstock et al, 1977b). However, with affinity chromatography for antigen isolation, the antiserum developed is often monospecific, as verified by immunoelectrophoresis, which is similar to the results with human protein (Blumenstock et al, 1979;Saba et al, 1978b). …”

Expression Profiling of Galectin-3-Depleted Melanoma Cells Reveals its Major Role in Melanoma Cell Plasticity and Vasculogenic Mimicry

“…Fibronectin is involved in cell adhesion and migration processes, including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. [65][66][67][68] Interference with tumor cell binding to fibronectin substrate interferes with cell functions such as adhesiveness, motility, and invasiveness in the cellular adhesive process of metastasis. 67 EDG-1 protein is also highly expressed in endothelial cells, where it binds the ligand sphingosine-1-phosphate, and is suggested to be involved in the processes that regulate differentiation of endothelial cells.…”

The role of the spleen in experimental pneumococcal bacteremia.

“…However, we were unable to observe any differences between Ty 7 and Ty 12 in their ability to cause complement depletion either in vivo or in vitro. It is possible that other opsonins, such as fibronectin (37), are involved, or even that metabolic differences among the Pn, such as the rate of division in vivo, are important factors in the way the bacteria interact with the RES. Nonetheless, these experiments indicate a key role for the spleen in the clearance of virulent or poorly opsonized bacteria.…”