Physicochemical studies on the interaction of gold(III) trichlorophenanthridine complex with calf thymus DNA

Moradi, Sasan; Ajloo, Davood; Lashkarbolouki, Taghi; Alizadeh, Robabeh; Saboury, Ali Akbar

doi:10.1007/s00706-013-1032-5

Cited by 11 publications

(3 citation statements)

References 27 publications

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“…Hyperchromism occurs due to the breakage of the secondary structure of DNA after the interaction with compounds and DNA, which derogates the interaction forces maintaining the DNA double-helix structure. Thus, the solution had many bases in free helix form and did not form hydrogen bonds with complementary bases, causing heightened absorbance [46,47]. The obtained results clearly show a groove-binding mode of binding of the free ligands and VO-PNZ complex to DNA.…”

Section: Kinetic Studies Of the Tgamentioning

confidence: 83%

Theoretical Investigation by DFT and Molecular Docking of Synthesized Oxidovanadium(IV)-Based Imidazole Drug Complexes as Promising Anticancer Agents

et al. 2022

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Vanadium compounds have been set in various fields as anticancer, anti-diabetic, anti-parasitic, anti-viral, and anti-bacterial agents. This study reports the synthesis and structural characterization of oxidovanadium(IV)-based imidazole drug complexes by the elemental analyzer, molar conductance, magnetic moment, spectroscopic techniques, as well as thermal analysis. The obtained geometries were studied theoretically using density functional theory (DFT) under the B3LYP level. The DNA-binding nature of the ligands and their synthesized complexes has been studied by the electronic absorption titrations method. The biological studies were carried with in-vivo assays and the molecular docking method. The EPR spectra asserted the geometry around the vanadium center to be a square pyramid for metal complexes. The geometries have been confirmed using DFT under the B3LYP level. Moreover, the quantum parameters proposed promising bioactivity of the oxidovanadium(IV) complexes. The results of the DNA-binding revealed that the investigated complexes bind to DNA via non-covalent mode, and the intrinsic binding constant (Kb) value for the [VO(SO4)(MNZ)2] H2O complex was promising, which was 2.0 × 106 M−1. Additionally, the cytotoxic activity of the synthesized complexes exhibited good inhibition toward both hepatocellular carcinoma (HepG-2) and human breast cancer (HCF-7) cell lines. The results of molecular docking displayed good correlations with experimental cytotoxicity findings. Therefore, these findings suggest that our synthesized complexes can be introduced as effective anticancer agents.

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Section: Kinetic Studies Of the Tgamentioning

confidence: 83%

Theoretical Investigation by DFT and Molecular Docking of Synthesized Oxidovanadium(IV)-Based Imidazole Drug Complexes as Promising Anticancer Agents

et al. 2022

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“…As is already known, there are two possible ways of binding to DNA, covalent (replacement of a labile ligand with, for example, N7 atom of guanine) or non‐covalent (intercalation, electrostatic, or groove binding) interactions. [ 65,66 ]…”

Section: Resultsmentioning

confidence: 99%

“…covalent (replacement of a labile ligand with, for example, N7 atom of guanine) or non-covalent (intercalation, electrostatic, or groove binding) interactions. [65,66] The binding affinity of the complex was evaluated by following the changes in the spectra of the complex upon increasing the concentration of the CT DNA (Figure S8, SI). The addition of a certain amount of CT DNA caused a moderate hyperchromic effect in the main absorption band.…”

Section: Absorption Spectroscopic Studiesmentioning

confidence: 99%

New gold(III) chlorophenyl terpyridine complex: Biomolecular interactions and anticancer activity against human oral squamous cell carcinoma

Radisavljević

Kesić

Ćoćić

et al. 2022

Applied Organom Chemis

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In this work we synthesized new monofunctional gold(III) complex [Au(Cl‐Ph‐tpy)Cl]Cl2 (Cl‐Ph‐tpy = 4′‐[4‐chlorophenyl]‐2,2′:6′, 2″‐terpyridine). This complex was characterized by UV–Vis, NMR, IR, and ESI‐MS spectrometry. The kinetic study of the substitution reactions of the Au‐Cl‐Ph‐tpy complex with biomolecules showed that the rate constants depend on the nature of the entering nucleophile. Based on the calculated values of entropy (∆H≠ > 0) and enthalpy (∆S≠ < 0) the proposed substitution mechanism is associative. Additionally, the relative stability and thermodynamic properties of Au‐Cl‐Ph‐tpy complex were compared with the analogue Au‐tpy complex by the B3LYP/def2‐svp method. DNA/BSA binding studies showed that Au‐Cl‐Ph‐tpy complex interacts with CT DNA through the intercalation and moderately quenches the fluorescence of tryptophan residues in serum albumin (BSA). Molecular docking confirmed results obtained by spectroscopic experiments and suggested site I (subdomain IIA) for binding of Au complex to BSA. We demonstrated that the Au chlorophenyl terpyridine complex possessed significant in vitro cytotoxic activity against human oral squamous carcinoma cells (CAL‐27), induced apoptosis, inhibited proliferation of CAL‐27 cells, and induced cell cycle disturbance. Treatment of CAL‐27 cells with the Au complex enhanced expression of cyclin‐dependent kinase inhibitors p21 and p27, resulting in cell cycle arrest in the G1 phase, reduced the percentage of CAL‐27 cells in S phase and decreased expression of Ki‐67. Additionally, Au complex reduced expression of phosphorylated STAT3 and downstream regulated molecules associated with cancer stemness, NANOG, and Sox2 protein.

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Changes of lipidemia after one month of creatine supplementation

2014

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Physicochemical studies on the interaction of gold(III) trichlorophenanthridine complex with calf thymus DNA

Cited by 11 publications

References 27 publications

Theoretical Investigation by DFT and Molecular Docking of Synthesized Oxidovanadium(IV)-Based Imidazole Drug Complexes as Promising Anticancer Agents

Theoretical Investigation by DFT and Molecular Docking of Synthesized Oxidovanadium(IV)-Based Imidazole Drug Complexes as Promising Anticancer Agents

New gold(III) chlorophenyl terpyridine complex: Biomolecular interactions and anticancer activity against human oral squamous cell carcinoma

Changes of lipidemia after one month of creatine supplementation

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