Physicochemical Properties of Protoporphyrin IX by Metal Ions in Acetonitrile-Water Mixture Solution

Bark, Ki‐Min; Yang, Jeong-Im; Lee, Ho-Suk; Lee, Jee-Bum; Park, Chul Ho; Park, Hyoung-Ryun

doi:10.5012/bkcs.2010.31.6.1633

Cited by 4 publications

(2 citation statements)

References 19 publications

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“…We measured fluorescence on the same instrument by exciting at 399 nm and measuring emission at 450 nm to 699 nm with 1 nm step. The spectra were compared with those shown in Bark et al (2010) .…”

Section: Methodsmentioning

confidence: 99%

Systematic bacterialization of yeast genes identifies a near-universally swappable pathway

Kachroo

Laurent

Akhmetov

et al. 2017

eLife

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Eukaryotes and prokaryotes last shared a common ancestor ~2 billion years ago, and while many present-day genes in these lineages predate this divergence, the extent to which these genes still perform their ancestral functions is largely unknown. To test principles governing retention of ancient function, we asked if prokaryotic genes could replace their essential eukaryotic orthologs. We systematically replaced essential genes in yeast by their 1:1 orthologs from Escherichia coli. After accounting for mitochondrial localization and alternative start codons, 31 out of 51 bacterial genes tested (61%) could complement a lethal growth defect and replace their yeast orthologs with minimal effects on growth rate. Replaceability was determined on a pathway-by-pathway basis; codon usage, abundance, and sequence similarity contributed predictive power. The heme biosynthesis pathway was particularly amenable to inter-kingdom exchange, with each yeast enzyme replaceable by its bacterial, human, or plant ortholog, suggesting it as a near-universally swappable pathway.DOI: http://dx.doi.org/10.7554/eLife.25093.001

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Section: Methodsmentioning

confidence: 99%

Systematic bacterialization of yeast genes identifies a near-universally swappable pathway

Kachroo

Laurent

Akhmetov

et al. 2017

eLife

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“…This further confirms that protoporphyrins have been metalized. [28] The connection of Ni to the N-aromatic ring of protoporphyrin was proved by spectral characterization FTIR. As shown in Figure 2a, significant changes in the position of peak displacements for carbonyl group stretching (1708 to 1628 cm À 1 ) and CÀ N stretching (1111 to 1092 cm À 1 ) vibrations were observed [29] Moreover, EDX analysis was used to confirm the metalation of protoporphyrin which contained all of the predicted constituents, (Figure 2b).…”

Section: Characterization Of Ni-ppix Complexmentioning

confidence: 99%

Porphysome Engineered With Specific Protein Binding Sites as a Multimodal Theranostic Nanocarrier for Targeted Protein Delivery

Ayyari,

Vaezi,

Ashin

et al. 2024

Chemistry & Biodiversity

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The immobilization of proteins on the surface of carriers is challenging due to the loss of protein structure and function in this process. Here, we report the development of the protein immobilization on the surface of the metallated‐porphyrin complex in the porphysome nanocarrier. The conjugated Ni‐porphyrin to fatty acid (as a tail) has been synthesized and independently placed at the depth of the bilayer center of Dipalmitoylphosphatidylcholine (DPPC) in which the Ni‐porphyrin was at the polar region of the membrane and is thus superficial. This porphysome (DPPC: Ni‐porphyrin, 4:1 mole ratio) was formed by supramolecular self‐assembly with a diameter of 173±7 nm and zeta potential ‐8.5±3.4 mv, which exhibited no significant toxicity at the experimental concentrations and acceptable cellular uptake on MCF‐7 cells. The physicochemical properties and specific protein binding sites of the firefly luciferase as a model protein into the porphysome (1:2 mole ratio) show the conjugation efficiency about 80% and the conformation of protein was completely maintained. Furthermore, bioluminescence assay and SDS‐PAGE confirmed the preservation of protein function. The stabilized platform of porphyrin‐lipid structure can potentially improve the efficacy of protein functionality for a particular display, shifting porphysomes from a simple carrier to a therapeutic agent.

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Hyperspectral Determination of Fluorescence Wavebands for Multispectral Imaging Detection of Multiple Animal Fecal Species Contaminations on Romaine Lettuce

Cho

Kim

Kim³

et al. 2018

Food Bioprocess Technol

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Physicochemical Properties of Protoporphyrin IX by Metal Ions in Acetonitrile-Water Mixture Solution

Abstract: The UV-vis absorption spectrum of protoporphyrin IX shows a very sharp and strong absorption maximum peak at 398 nm in acetonitrile-water mixture solution

Cited by 4 publications

References 19 publications

Systematic bacterialization of yeast genes identifies a near-universally swappable pathway

Systematic bacterialization of yeast genes identifies a near-universally swappable pathway

Porphysome Engineered With Specific Protein Binding Sites as a Multimodal Theranostic Nanocarrier for Targeted Protein Delivery

Hyperspectral Determination of Fluorescence Wavebands for Multispectral Imaging Detection of Multiple Animal Fecal Species Contaminations on Romaine Lettuce

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