Physicochemical Characterization of the First World Health Organization International Standard for Low Molecular Weight Heparin Derivatives

Physicochemical Characterization of Low Molecular Weight Heparin

“…Another feature of the NMR spectra is the absence of the characteristic minor signals at 6 5.9 and 5.6 which would be due to the presence of a proportion of a,@-unsaturated acid produced by @-elimination in esterified heparin. 18 The characteristic signals in the 6 5.5-4.5 region for the nitrous acid-prepared material are due to end residues of anhydromannitol that are produced by deamination and subsequent depolymerization .18*23 Raman Spectra-The Raman spectra of the two preparations of low molecular weight heparin are shown in Figure 2A. The major peaks at 1040 and 1060 cm-1 are produced by the symmetric S-0 stretching of the N-and 0-sulfate residues, respectively.…”

Gradient Polyacrylamide Gel Electrophoresis for Determination of Molecular Weights of Heparin Preparations and Low-Molecular-Weight Heparin Derivatives

“…A physiochemical characterization of this WHO standard has recently been published. 8 The M, values of LMW heparin derivatives affect their biological activity and bioavailability. @ The M, value of heparin has been estimated by using several physical methods.…”