Physicochemical characterization of dual action liposomal formulations: anticancer and antimicrobial

Abstract

Background: Cancer till date remains one of the world's most life threatening disease accompanied by risk of secondary infections. Therefore formulations carrying anticancer drugs which can also decrease the risk of secondary infection are inevitable. Chemotherapeutic drug doxorubicin along with flavonoids quercetin and epigallocatechin gallate (EGCG) is simultaneously loaded on liposomal formulation exploiting the amphiphilic property of the liposomes. Results: Atomic force microscope imaging reveal the size of liposomal formulation loaded with doxorubicin, quercetin and EGCG to be greater than void liposome confirming the presence of drugs. Liposomal stability is improved by PEGylation; adding to the drug release time in vitro. The charge of phosphatidylcholine is rendered positive by coating the formulation with histone. The average size of the formulation is 342 nm. The encapsulation efficiency of doxorubicin, quercetin and EGCG is found to be 65.8%, 96.8% and 98% respectively. The above formulation demonstrated both anticancer and antimicrobial activity. Conclusion: The formulation will provide dual anticancer and antimicrobial therapy thereby evading secondary infection in cancer patients along with chemotherapy.

Formulation and characterization of Ciprofloxacin encapsulated liposomes: in vitro antimicrobial activity against multi drug resistant Salmonella typhi

“…Similar explanation of dark and light area were explained in the previous studies (Ruozi et al, 2011 ;Topal et al, 2018). In one of the previous study the flattening of liposome was observed which is thought to be considered because of their high surface density preventing the tip from direct contact with the liposomal surface (Das et al, 2019). Moreover, AFM was being used in current study to examine the lipid bilayers and to observe the significant differences of liposomes and ciprofloxacin encapsulated liposomes.…”

Liposomal Encapsulation of Phenolic Compounds for Augmentation of Bio‐Efficacy: A Review

“…Liposome co‐loading resveratrol and curcumin exhibited greater EE (80.42 %) compared to single loaded PCs (resveratrol loaded liposome: 59.46 % and curcumin loaded liposome: 68.16 %). Das et al [60] . also obtained a high EE of 96.8 % and 98 % for quercetin and EGCE, respectively, using the co‐encapsulation method.…”

“…also attempted a similar co‐encapsulation of curcumin and resveratrol to enhance the stability, antioxidant efficacy, and encapsulation efficacy of the system. Again, Das et al [60] . have simultaneously loaded polyphenol quercetin and EGCG along with anticancer drug doxorubicin to enhance the effectiveness of the total system.…”

“…have also developed stealth liposomal systems using PEG to deliver flavonoid quercetin. Das et al [60] . have additionally coated PEGylated liposome formulations with histone during co‐encapsulation of quercetin, EGCG, and doxorubicin.…”

“…The order of antioxidant activity provided as: EGCG loaded liposomes (IC 50 : 24.71±1.03 mg/L)<Quercetin loaded liposomes (IC 50 : 23.13±0.99 mg/L)<EGCG/quercetin loaded liposomes (IC 50 : 21.68±0.94 mg/L). However, slight modification by introducing doxorubicin in the co‐loading system along with EGCG and quercetin, by Das et al., [60] resulted in interesting observations. Simultaneous loading of quercetin, EGCG, and doxorubicin in histone‐coated PEGylated liposome showed effective anticancer activity against leukemic cells (K562) with 93.77 % apoptotic population, compared to unloaded (apoptotic population: 0.14 %) and quercetin loaded liposome (apoptotic population: 63.87 %).…”