Physicochemical characterization of chitosan derivatives

Seoane, Annabelle; Abuín, Alicia García; Gómez‐Díaz, Diego; Dafonte, José Manuel Navaza

doi:10.1080/19476337.2012.722565

Cited by 16 publications

(13 citation statements)

References 34 publications

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“…This result was in cope with the previously reported works on CHN analysis of chitosan. 30 The ATR spectra of commercial chitosan and isolated chitosan are shown in Figure 1. Nine major peaks were obtained for chitosan prepared from shrimp shell and were almost similar as that of commercial chitosan.…”

Section: Resultsmentioning

confidence: 99%

Evaluating the biocompatibility of marine-derived chitosan–collagen polymeric blends for biomedical applications

Raghavankutty

Jose

Mohsin

et al. 2017

Journal of Bioactive and Compatible Polymers

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The capability of biomaterials such as chitosan and collagen to support cell growth and proliferation makes them promising candidates in biomedical applications. Chitosan and collagen from marine world have already been proved to be better alternatives to those from terrestrial world. In the current study, chitosan and collagen were isolated from shrimp shell and fish skin, respectively. The polymers were characterized by ultraviolet-visible spectra analysis, Fourier transform infraredattenuated total reflectance analysis, CHN analysis, and sodium dodedcyl sulfate polyacrylamide gel electrophoresis analysis. Interpenetrating blends of these polymers were synthesized in the form of films in two different ratios. Glutaraldehyde was used as an additional cross-linker to provide more stability to the blends. The polymeric blends were also characterized by Fourier transform infrared-attenuated total reflectance, scanning electron microscopy analysis, and swelling studies. The biocompatibility evaluation included hemocompatibility and cytocompatibility studies. Fourier transform infrared-attenuated total reflectance analysis of films confirmed the presence of characteristic functional groups and molecular interactions of the two polymers in the two blends. Homogenous blending of the two biopolymers in both film compositions was confirmed by the smooth surface images in scanning electron microscopy analysis. The swelling study revealed that both the films can effectively transfer water across it, hence nutrients and waste materials. During hemocompatibility evaluations, no red blood cell aggregation was observed and both the films adsorbed plasma proteins, predominantly albumin, when they made contact with blood. Although one of the films showed slightly higher hemolysis, the value was within the acceptable range. More than 90% viability obtained in 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay shows the non-toxic nature of the two films. No sign of morphological changes

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Section: Resultsmentioning

confidence: 99%

Evaluating the biocompatibility of marine-derived chitosan–collagen polymeric blends for biomedical applications

Raghavankutty

Jose

Mohsin

et al. 2017

Journal of Bioactive and Compatible Polymers

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“…The viscosity average molecular weight ( M v ) of sulfate and phosphate functionalized derivatives and native chitosan was estimated based on the Mark–Houwink equation. Mark–Houwink constants, K and a , were considered to be 8.2 × 10 –4 and 0.72, respectively, for CH in a 0.3 M AcOH–0.2 M NaOAc solvent system, 1.75 × 10 –5 and 0.93, respectively, for CS in a 0.1 M NaCl solvent system, whereas they were 1.81 × 10 –5 and 0.98, respectively, for CP in 0.1 M AcOH–0.2 M NaCl …”

Section: Methodsmentioning

confidence: 99%

Osteochondral Defects Healing Using Extracellular Matrix Mimetic Phosphate/Sulfate Decorated GAGs-Agarose Gel and Quantitative Micro-CT Evaluation

Kapat¹,

Rameshbabu²,

Maity

et al. 2018

ACS Biomater. Sci. Eng.

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Tissue engineering has a major emphasis in creating tissue specific extracellular ambiance by altering chemical functionalities of scaffold materials. Heterogeneity of osteochondral tissue necessitates tailorable bone and cartilage specific extracellular environment. Carboxylate- and sulfate-functionalized glycosaminoglycans (GAGs) in cartilage extracellular matrix (ECM) create an acidic ambience to support chondrogenic activity, whereas phosphate-rich environment in bone enables chelation of calcium leading to the formation of mineralized matrix along with an alkaline environment to support osteogenesis. In this study, chitosan, a naturally occurring GAGs, was functionalized with phosphate/sulfate groups analogous to bone/cartilage ECM and incorporated in thermogelling agarose hydrogel for delivery to osteochondral defects. In vitro studies revealed significantly higher adhesion and proliferation of adipose derived mesenchymal stem cells (ADMSCs) with blended hydrogels as compared to that of native agarose. Cell differentiation and RT-PCR studies of the phosphorylated hydrogels revealed higher osteogenic potential, while sulfated hydrogels demonstrated enhanced chondrogenic activity in comparison to agarose. Recovery of osteochondral defects after delivery of the thermoresponsive agarose-based hydrogels decorated with phosphorylated derivatives showed significantly higher bone formation. On the other hand, cartilage formation was significant with chitosan sulfate decorated hydrogels. The study highlights the role of chitosan derivatives in osteochondral defect healing, especially phosphorylated ones as bone promoter, whereas sulfated ones act as cartilage enhancer, which was quantitatively distinguished through micro-CT-based noninvasive imaging and analysis.

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“…Two transition points, corresponding to excess HCl (x) and protonated chitosan (y) were taken from the titration curve. [26,27] The difference in the amount of NaOH consumed is equivalent to the amount of free deacetylated amino groups and it is given by equation (2).…”

Section: Estimation Of Degree Of Deacetylation (Ds Da )mentioning

confidence: 99%

Antibacterial Polyelectrolytic chitosan derivatives conjugated natural rubber latex films with minimized bacterial adhesion

Arakkal

Aazem

Honey

et al. 2020

J of Applied Polymer Sci

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Water soluble polyelectrolytic derivatives of chitosan (CDs) with carboxyl (COO −) and quaternary ammonium (NH 4 +) groups were synthesized and characterized by NMR, FT-IR, TGA and zeta potential analyses; while degree of substitution was determined by potentiometric titrations. Both CDs in the as-synthesized form exhibited antibacterial zones against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). The CD conjugated vulcanized natural rubber latex (NRL) films were investigated for understanding the level of bacterial adhesion and bactericidal properties as a function of the polyelectrolytic groups present. NRL Films modified at ambient conditions were found effective against Pseudomonas aeruginosa (P. aeruginosa). The biofilm assay results demonstrate that CD conjugated samples exhibit significantly reduced adhesion (63.8%) against P. aeruginosa. Further, alterations observed in cellular morphology of attached bacteria indicate that the modified surface of films is bacteriocidal while presence of significantly higher level of extra cellular polymer substances (EPS) were detected on control NRL films.

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Physicochemical characterization of chitosan derivatives

Cited by 16 publications

References 34 publications

Evaluating the biocompatibility of marine-derived chitosan–collagen polymeric blends for biomedical applications

Evaluating the biocompatibility of marine-derived chitosan–collagen polymeric blends for biomedical applications

Osteochondral Defects Healing Using Extracellular Matrix Mimetic Phosphate/Sulfate Decorated GAGs-Agarose Gel and Quantitative Micro-CT Evaluation

Antibacterial Polyelectrolytic chitosan derivatives conjugated natural rubber latex films with minimized bacterial adhesion

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