PHYSICO-CHEMICAL ASSESSMENT OF NATURAL SWEETENERS STEVIOSIDES PRODUCED FROM STEVIA Rebudiana bertoni PLANT

Abstract

Stevia plants are a good source of carbohydrates (61.93 % d.w.), protein (11.41 % d.w.), crude fiber (15.52 % d.w.), minerals (K, 21.15; Ca, 17.7; Na, 14.93 & Mg, 3.26 mg/100g d.w. and Cu, 0.73; Mn, 2.89; Fe, 5.89 & Zn, 1.26 mg/100g d.w.) also essential amino acids were found in amounts higher than those recommended by FAO and WHO for adults as well as non-essential amino acids. Stevioside was extracted and purified from the dried Stevia leaves by three methods. The first one, extraction by hot water (65 °C) at different ratios of leaves to water (1:15 to 1:75). The optimum ratio was 1:35 in which the maximum stevioside content was obtained (7.53 %), recovery of stevioside was 80.21 % and purity related to depigmentation was 97.56 %. The second method, extraction by methanol at ratio 4:1 methanol/leaves and purification, recovery of stevioside was 94.90 % and purity was 81.53 %. The third method, extraction by mixture of methanol/water (4:1) and purification, recovery of stevioside and purity were 92.34 and 79.81 %, respectively. Physical and chemical characteristics of the extracted stevioside were studied. Stevioside extractions were analyzed by HPLC in which the highest amount of stevioside (23.20 %) was obtained in methanol extract. Physical, chemical and organolyptic characteristics of extractions indicate that this sweetener has been applied as substitutes for sucrose in different drinks and baking products.

Relationship of colour with the phytocompounds present in Cornus mas cultivars

“…The assay was conducted according to the Abou-Arab et al method. [10] The obtained extracts were subjected to multi-wavelength absorbance measurement in the SPECORD S40 apparatus (Analytic Jena, Germany). Absorbance was read for the following wavelengths: 440 nm (A 440 ); 644 nm (A 644 ) and 662 nm (A 662 ).…”

Stevia rebaudiana Bertoni - chemical composition and functional properties

“…Carbohydrates are the main sources of energy and they are found as structural components of cellular elements (Lemus-Modaca et al, 2012). Carbohydrate contents in dry leaves of sweetleaf ranged from 35.2 to 61.9 g•100 g -1 product (Abou-Arab et al, 2010;Boonkaewwan et al, 2006). Their positive action is connected with prebiotic properties promoting proliferation of benefi cial intestinal microfl ora.…”

“…Fat content in dry matter of stevia leaves amounts to 1.9-4.34 g•100 g -1 d.m. (Abou-Arab et al, 2010;Siddique et al, 2014). The chemical composition of this plant is also affected by the method of leaf drying (Gasmalla et al, 2014).…”

Sensory Attributes, Microbial Activity, Fatty Acid Composition and Meat Quality Traits of Hanwoo Cattle Fed a Diet Supplemented with Stevioside and Organic Selenium

“…Previous studies [ 8 , 9 ] reported that the stevioside exhibits numerous immunoregulation activities and has antibacterial properties, anti-inflammation properties, and maintenance of the blood lipid content. Previous studies showed that the stevia extract contains amino acids, polyphenols, and flavonoids and has antioxidant properties [ 10 , 11 , 12 , 13 ]. A few studies have been conducted on nonruminants, including pigs and poultry [ 14 , 15 ].…”