Physical, mechanical, and biological properties of collagen membranes for guided bone regeneration: a comparative in vitro study

Abstract

Background To provide a reference for clinical selection of collagen membranes by analyzing the properties of three kinds of collagen membranes widely used in clinics: Bio-Gide membrane from porcine dermis (PD), Heal-All membrane from bovine dermis (BD), and Lyoplant membrane from bovine pericardium (BP). Methods The barrier function of three kinds of collagen membranes were evaluated by testing the surface morphology, mechanical properties, hydrophilicity, and degradation rate of collagen membranes in collagenase and artificial saliva. In addition, the bioactivity of each collagen membrane as well as the proliferation and osteogenesis of MC3T3-E1 cells were evaluated. Mass spectrometry was also used to analyze the degradation products. Results The BP membrane had the highest tensile strength and Young’s modulus as well as the largest water contact angle. The PD membrane exhibited the highest elongation at break, the smallest water contact angle, and the lowest degradation weight loss. The BD membrane had the highest degradation weight loss, the highest number of proteins in its degradation product, the strongest effect on the proliferation of MC3T3-E1 cells, and the highest expression level of osteogenic genes. Conclusions The PD membrane is the best choice for shaping and maintenance time, while the BD membrane is good for osteogenesis, and the BP membrane is suitable for spatial maintenance. To meet the clinical requirements of guided bone regeneration, using two different kinds of collagen membranes concurrently to exert their respective advantages is an option worth considering.

Development, Characterization, and Antibacterial Analysis of the Selenium-Doped Bio-Glass-Collagen-Gelatin Composite Scaffold for Guided Bone Regeneration

“…The surface roughness due to lamellar structure and fibrillar arrangements could be attributed to the presence of collagen and gelatin in the membrane. Similar surface properties were observed in studies analyzing the collagen-based GBR membranes revealing marked differences in the microstructures with loosely arranged bundles of collagen [18]. As collagen is a ubiquitous compound present in the extracellular matrix of human tissues, it plays a significant role in biomimicry during the healing process leading to tissue regeneration.…”

Assembled collagen films modified using polyacrylic acid with improved mechanical properties via mineralization

“…4F, PAA–COL + CPAM–SBF has a higher strength and modulus than those of other collagen-based materials based on our knowledge. 64–76…”

Optimization of Polydimethylsiloxane-Modified Composite Synthesis and Its Impact on Collagen Interactions: Perspectives for Biomedical Applications

“…The finding that collagen retains its native conformation on the PDMS-modified silica composite’s surface is particularly relevant. This suggests that the composite does not denature the protein, which is crucial for maintaining its biological functionality in potential applications like guided bone regeneration or wound healing, where the bioactivity of collagen is a key factor [ 23 ]. The results are also supported by the fluorescence microscopy observations, which provided visual confirmation of the protein’s presence and distribution on the composite’s surface.…”