Physical Mapping of Amplified Copies of the 5-Enolpyruvylshikimate-3-Phosphate Synthase Gene in Glyphosate-Resistant <i>Amaranthus tuberculatus</i>

Dillon, Andrew J.; Varanasi, Vijay K.; Danilova, Tatiana; Koo, Dal Hoe; Nakka, Sridevi; Peterson, Dallas E.; Tranel, Patrick J.; Friebe, Bernd; Gill, Bikram S.; Jugulam, Mithila

doi:10.1104/pp.16.01427

Cited by 56 publications

(68 citation statements)

References 49 publications

(78 reference statements)

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“…Thus, aneuploidy is likely a form of stress-inducible 198 mutation in eukaryotes (Chen et al, 2012) but genome adaptation through aneuploidy may also 199 occur spontaneously. This, and our earlier results (Dillon et al, 2017), are the first reports of spindle assembly checkpoint and chromatid cohesion are known to cause aneuploidy (Gordon et 207 al., 2012). Chromosome loss after polyploidization also is reported to lead to high levels of 208 aneuploidy in Drosophila rectal cells (Schoenfelder et al, 2014).…”

supporting

confidence: 51%

“…J.D.Sauer (Dillon et al, 2017). Plants 97 with the extra chromosome were correlated with increased expression of the EPSPS gene (Dillon 98 et al, 2017).…”

mentioning

confidence: 99%

“…Production of F 1 progeny by crossing glyphosate susceptible (GS) and GR A. tuberculatus Fluorescence in situ hybridization and immunofluorescence 327 328 Molecular cytogenetic mapping of EPSPS gene and telomeric DNA followed published 329 protocols (Koo and Jiang, 2009;Dillon et al 2017). To prepare the EPSPS FISH probe, the were carried out as described (Jin et al, 2004 copies had 2n=32 + 1 ECCAE (Fig.…”

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confidence: 99%

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Gene Duplication and Aneuploidy Trigger Rapid Evolution of Herbicide Resistance in Common Waterhemp

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confidence: 51%

“…J.D.Sauer (Dillon et al, 2017). Plants 97 with the extra chromosome were correlated with increased expression of the EPSPS gene (Dillon 98 et al, 2017).…”

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confidence: 99%

mentioning

confidence: 99%

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Gene Duplication and Aneuploidy Trigger Rapid Evolution of Herbicide Resistance in Common Waterhemp

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“…Therefore, detoxification or metabolism of auxinic herbicides via cyt-P450 can be considered as the possible mechanism involved in the resistance for the studied biotypes. Shikimate accumulation is a known marker of EPSPS inhibition in populations with sensitivity to glyphosate [27]. The inhibition of the shikimic acid metabolic pathway were confirmed in all biotypes of A. hybridus except in SJT1 biotype.…”

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confidence: 70%

First Report of Amaranthus hybridus with Multiple Resistance to 2,4-D, Dicamba, and Glyphosate

et al. 2018

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In many countries, Amaranthus hybridus is a widespread weed in agricultural systems. The high prolificacy and invasive capacity as well as the resistance of some biotypes to herbicides are among the complications of handling this weed. This paper reports on the first A. hybridus biotypes with resistance to auxinic herbicides and multiple resistance to auxinic herbicides and the EPSPs inhibitor, glyphosate. Several dose response assays were carried out to determine and compare sensitivity of six population of A. hybridus to glyphosate, 2,4-D, and dicamba. In addition, shikimic acid accumulation and piperonil butoxide effects on 2,4-D and dicamba metabolism were tested in the same populations. The results showed four populations were resistant to dicamba and three of these were also resistant to 2,4-D, while only one population was resistant to glyphosate. The glyphosate-resistant population also showed multiple resistance to auxinic herbicides. Pretreatment with piperonil butoxide (PBO) followed by 2,4-D or dicamba resulted in the death of all individual weeds independent of herbicide or population.

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“…All other ALS‐resistant plants had only the Trp574Leu mutation. Should there be more resistant plants with increased ALS gene copies in the future, gene amplification should be verified using southern blotting or fluorescence in situ hybridization as done in the case of EPSPS . Recently in Japan, one accession of shortawn foxtail ( Alopecurus aequalis var.…”

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confidence: 99%

Target‐site mutation accumulation among ALS inhibitor‐resistant Palmer amaranth

Singh

Salas-Perez

et al. 2018

Pest Management Science

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BACKGROUND Palmer amaranth (Amaranthus palmeri S. Wats) is one of the most common and troublesome weeds in the USA. Palmer amaranth resistance to acetolactate synthase (ALS) inhibitors is widespread in the USA, as in Arkansas. The cross‐resistance patterns and mechanism of resistance are not known. Experiments were conducted to determine cross‐resistance to ALS inhibitors and identify target‐site mutations in 20 Palmer amaranth localities from 13 counties in Arkansas. RESULTS All Palmer amaranth localities tested had plants cross‐resistant to imazethapyr, flumetsulam, primisulfuron, pyrithiobac and trifloxysulfuron. The dose of trifloxysulfuron that caused 50% control was 21–56‐fold greater for resistant accessions than for susceptible ones. All but three resistant plants analyzed had one or two relative copies of ALS; one plant had seven relative copies. All resistant plants tested (18 localities) carried the Trp574Leu mutation, which is known to confer broad resistance to ALS inhibitors, supporting the cross‐resistance pattern observed. Besides the Trp574Leu mutation, 30% of localities had individuals with one additional resistance‐conferring mutation including Ala122Thr, Pro197Ala or Ser653Asn. CONCLUSION The Trp574Leu mutation in ALS is the primary mechanism of resistance to ALS inhibitors in Palmer amaranth from Arkansas, USA. In some localities, multiple mutations have accumulated in one plant. All localities tested contained plants with resistance to five families of ALS inhibitors. Localities with extremely high resistance to ALS inhibitors, and those outside the subset we studied, may harbor non‐target site resistance mechanisms. ALS inhibitors are generally no longer effective on Palmer amaranth in these localities from the US mid‐south. © 2018 Society of Chemical Industry

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Physical Mapping of Amplified Copies of the 5-Enolpyruvylshikimate-3-Phosphate Synthase Gene in Glyphosate-Resistant Amaranthus tuberculatus

Cited by 56 publications

References 49 publications

Gene Duplication and Aneuploidy Trigger Rapid Evolution of Herbicide Resistance in Common Waterhemp

Gene Duplication and Aneuploidy Trigger Rapid Evolution of Herbicide Resistance in Common Waterhemp

First Report of Amaranthus hybridus with Multiple Resistance to 2,4-D, Dicamba, and Glyphosate

Target‐site mutation accumulation among ALS inhibitor‐resistant Palmer amaranth

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