Physalin F, a seco-steroid from Physalis angulata L., has immunosuppressive activity in peripheral blood mononuclear cells from patients with HTLV1-associated myelopathy

Pinto, Lorena Ana; Meira, Cássio Santana; Villarreal, Cristiane Flora; Vannier-Santos, Marcos A.; Souza, Claudia Valeria Campos de; Ribeiro, Ícaro José Santos; Tomassini, Therezinha Coelho Barbosa; Galvão–Castro, Bernardo; Soares, Milena Botelho Pereira; Grassi, Maria Fernanda Rios

doi:10.1016/j.biopha.2016.01.041

Cited by 31 publications

(24 citation statements)

References 30 publications

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“…Traditionally, this herb is considered to be antidiuretic, anti-inflammatory, antipyretic, and antinociceptive (6,7). Biological active extracts isolated from Physalis species are called physalins, which have been reported regulatory in immune system (8,9). Moreover, anti-inflammatory, antimalarial, and antinociceptive effects of physalins have been reported (10)(11)(12).…”

Section: Introductionmentioning

confidence: 99%

Physalin D inhibits RANKL-induced osteoclastogenesis and bone loss via regulating calcium signaling

Ding

Cui

et al. 2020

BMB Rep.

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We investigated the effects of physalin A, B, D, and F on osteoclastogenesis induced by receptor activator of nuclear factor B ligand (RANKL). The biological functions of different physalins were first predicted using an in silico bioinformatic tool (BATMAN-TCM). Afterwards, we tested cell viability and cell apoptosis rate to analyze the cytotoxicity of different physalins. We analyzed the inhibitory effects of physalins on RANKL-induced osteoclastogenesis from mouse bone-marrow macrophages (BMMs) using a tartrate-resistant acid phosphatase (TRAP) stain. We found that physalin D has the best selectivity index (SI) among all analyzed physalins. We then confirmed the inhibitory effects of physalin D on osteoclast maturation and function by immunostaining of F-actin and a pit-formation assay. On the molecular level, physalin D attenuated RANKLevoked intracellular calcium ([Ca(2＋)](i)) oscillation by inhibiting phosphorylation of phospholipase C2 (PLC2) and thus blocked the downstream activation of Ca2＋/calmodulindependent protein kinases (CaMK)IV and cAMP-responsive element-binding protein (CREB). An animal study showed that physalin D treatment rescues bone microarchitecture, prevents bone loss, and restores bone strength in a model of rapid bone loss induced by soluble RANKL. Taken together, these results suggest that physalin D inhibits RANKL-induced osteoclastogenesis and bone loss via suppressing the PLC2-CaMK-CREB pathway. [BMB Reports 2020; 53(3): 154-159] BMB Rep. 2020; 53(3): 154-159 www.bmbreports.org 157 http://bmbreports.org BMB Reports

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Section: Introductionmentioning

confidence: 99%

Physalin D inhibits RANKL-induced osteoclastogenesis and bone loss via regulating calcium signaling

Ding

Cui

et al. 2020

BMB Rep.

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“…It is noteworthy that this structure displayed cytoplasmic content including glycogen granules, presumably comprising autophagosomes. These membranes may be derived from ER, nucleoplasmic reticulum [37] or mitochondrial membranes [38].…”

Section: Discussionmentioning

confidence: 99%

Photodynamic therapy effect on the ultrastructure of Trichomonas vaginalis trophozoites and their effectiveness in experimentally infected animals

Fonseca

Gomes²,

Alacoque

et al. 2018

Preprint

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2 Background 3 Trichomonas vaginalis is an amitochondrial parasitic that causes human trichomoniasis, 4 the most common non-viral sexually transmitted infection in the world. The therapy of 5 choice is metronidazole (MTZ). Despite MTZ effectiveness, resistant cases are 6 becoming more frequent. Another point to emphasize are the side effects that may result 7 in treatment discontinuation, leading to further spread of infection and emergence of 8 resistant strains. This scenario reveals the need to develop new therapeutic options. 9 Photodynamic therapy (PDT) is an experimental treatment that involves the activation 10 of photosensitive substances and the generation of cytotoxic oxygen species and free 11 radicals to promote the selective destruction of target tissues. A previous study, from 12 our group, identified an excellent in vitro PDT activity using methylene blue and light 13 emitting diode against MTZ sensitive and resistant strains of T. vaginalis. The aim of 14 this study was to evaluate the efficacy of PDT in vivo and clarify its high 15 trichomonicidal potential by evaluating its action upon T. vaginalis trophozoites through 16 transmission electron microscopy (TEM). 17 18 Methodology 19 Seven-week-old female Balb/c mice were infected intravaginally with T. vaginalis 20 trophozoites. On the third day of infection, methylene blue was introduced into the 21 vaginal canal of the animals, which then received 68.1 J / cm 2 of radiation for 35.6 sec. 22 Control groups without infection and infected, treated with metronidazole were also 23 included for comparison. Twenty-four hours after treatment the vaginal canal of the 24 animals was scraped and the samples processed by the immunocytochemistry technique.3 3 25 After in vitro photodynamic treatment, T. vaginalis trophozoites were processed for 26 TEM. Ultrathin sections were collected in 400-mesh copper grids, contrasted with 5% 27 uranyl acetate and 3% lead citrate, in aqueous solutions for 20 and 5 min., respectively 28 and observed in a Jeol JEM 230 transmission electron microscope. 29 30 Results 31 TEM showed morphological changes such as centripetal displacement of organelles, 32 cannibalism, hydrogenosomal damage, intense cytoplasmic vacuolization, dilated 33 endoplasmic reticulum cisternae and membrane discontinuity, in both resistant and 34 sensitive strains, suggesting that trichomonicidal activity is mainly due to necrosis. 35 PDT significantly reduced infection in animals treated with a single therapy session, 36 compared to control groups, being statistically as efficient as MTZ. 37 38 Conclusions 39 Our results demonstrated high trichomonicidal activity of PDT with morphological 40 alterations compatible with necrosis. Therefore these results indicate that PDT 41 represents not only an alternative therapy for refractory trichomoniasis, but also 42 routinely for this important neglected parasitic disease.43 44

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“…investigated the effects of physalin F ( 221 ) on peripheral blood mononuclear cells (PBMC) of HAM/TSP subjects. It decreased the proliferation and cytokine production caused by HTLV‐1 infection (IC 50 = 0.97 ± 0.11 μ m ) as evaluated by 3H‐thymidine uptake, suggesting that 221 has an immunosuppressive activity in peripheral blood mononuclear cells from patients with HTLV1‐associated myelopathy …”

Section: Pharmacological Effectsmentioning

confidence: 99%

Withanolides from the genus Physalis: a review on their phytochemical and pharmacological aspects

Huang

et al. 2019

Journal of Pharmacy and Pharmacology

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Objectives Withanolides are a group of modified C28 ergostane‐type steroids with a C‐22, C‐26 δ‐lactone side chain or a C‐23, C‐26 γ‐lactone side chain. They enjoy a limited distribution in the plant kingdom and predominantly occur in several genera of Solanaceae. Of which, the genus Physalis is an important resource for this type of natural molecules. The present review aims to comprehensively illustrate the structural characteristics and classification of withanolides, and particularly focus on the progression on phytochemical and pharmacological aspects of withanolides from Physalis ranging from January 2015 to June 2019. Key findings Approximately 351 natural withanolides with novel and unique structures have so far been identified from genus Physalis, mainly isolated from the species of P. angulata and P. peruviana. Withanolides demonstrated diverse biological activity, such as anticancer, anti‐inflammatory, antimicrobial, immunoregulatory, trypanocidal and leishmanicidal activity. Their observed pharmacological functions supported the uses of Physalis species in traditional or folk medicines. Summary Due to their unique structure skeleton and potent bioactivities, withanolides are regarded to be promising drug candidates, particularly for developing anticancer and anti‐inflammatory agents. Further investigations for discovering novel withanolides of genus Physalis, exploiting their pharmacological values and evaluating their potency as therapeutic agents are significant work.

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Physalin F, a seco-steroid from Physalis angulata L., has immunosuppressive activity in peripheral blood mononuclear cells from patients with HTLV1-associated myelopathy

Cited by 31 publications

References 30 publications

Physalin D inhibits RANKL-induced osteoclastogenesis and bone loss via regulating calcium signaling

Physalin D inhibits RANKL-induced osteoclastogenesis and bone loss via regulating calcium signaling

Photodynamic therapy effect on the ultrastructure of Trichomonas vaginalis trophozoites and their effectiveness in experimentally infected animals

Withanolides from the genus Physalis: a review on their phytochemical and pharmacological aspects

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