Phylogeography and genetic structure of the Mediterranean killifish Aphanius fasciatus (Cyprinodontidae)

Triantafyllidis, Alexander; Leonardos, Ioannis; Bista, Iliana; Kyriazis, Ioannis D.; Stoumboudi, Maria Th.; Kappas, Ilias; Amat, Francisco; Abatzopoulos, Theodore J.

doi:10.1007/s00227-007-0760-7

Cited by 32 publications

(43 citation statements)

References 46 publications

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“…It tolerates a wide range of physico-chemical parameters, such as temperature (5-39ºC) and salinity (0-180 g/L). Although migration of individuals from one habitat to another is possible, it is considered a non-migratory species, as populations from relatively close habitats show evidences for genetic isolation ((Triantafyllidis et al, 2007) and references therein). Therefore, A. fasciatus represents an excellent sentinel species for analysis of environmental impact in coastal lagoons, estuaries and river deltas in the highly anthropized Mediterranean Sea.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of environmental impact on natural populations of the Mediterranean killifish Aphanius fasciatus by quantitative RNA biomarkers

Kessabi

Navarro

Casado

et al. 2010

Marine Environmental Research

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The Mediterranean killifish, Aphanius fasciatus (Valenciennes, 1821) represents an excellent sentinel species for analysis of environmental impact in coastal areas.Quantitative changes on liver mRNA levels of five stress-related genesmetallothionein (metal exposure), vitellogenin, (estrogenic effects), cytochrome P4501A (CYP1A, dioxin-like compounds), superoxide dismutase (oxidative stress) and HSP70 (general stress)-were tested as markers of exposure to pollutants both in natural populations and in experimental treatments with model effectors. Analysis of fish from a metal-contaminated site in the Tunisian coast (Sfax) indicated that fish presenting spinal deformities (eight times more frequent at this site than in reference sites) showed increased levels of hepatic CYP1A mRNA levels compared to non-deformed fish from the same site or from the reference Louza site, suggesting a combined impact by metals and organic pollutants. Due to the strategy used in their design, these quantitative RNA markers will likely be useful to analyze environmental impacts on other related fish species.

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Section: Introductionmentioning

confidence: 99%

Evaluation of environmental impact on natural populations of the Mediterranean killifish Aphanius fasciatus by quantitative RNA biomarkers

Kessabi

Navarro

Casado

et al. 2010

Marine Environmental Research

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“…Hrbek and Meyer (2003) found low genetic variation among A. fasciatus populations, despite its wide distribution and potential subjection to Mediterranean vicariant events, whereas other authors documented marked genetic structuring among populations of this species , Triantafyllidis et al 2007). Hrbek and Meyer (2003) explained this low genetic divergence of A. fasciatus by occasional migration events that prevented differentiation.…”

Section: Introductionmentioning

confidence: 92%

“…It is able to tolerate a wide range of physicochemical parameters, in particular temperature (5-39°C) and salinity (0-180 psu) (Triantafyllidis et al 2007). Due to its life history traits, such as large adhesive eggs, short generation time, high reproductive rate and, especially, the absence of larval dispersal stages, this species is considered to be an ideal organism for investigating the effects of genetic drift, selection and gene flow on population divergence (Triantafyllidis et al 2007). Furthermore, A. fasciatus has no commercial value, so the distribution of genotypes among populations has likely not been affected by human translocations.…”

Section: Introductionmentioning

confidence: 99%

“…Hrbek and Meyer (2003) explained this low genetic divergence of A. fasciatus by occasional migration events that prevented differentiation. Triantafyllidis et al (2007) analysed samples from 13 localities in Greece and Turkey and concluded that the Messinian Salinity Crisis was the most important event in shaping their recent population genetic structure. Bardakci et al (2004) investigated Anatolian Aphanius populations and revealed limited gene flow among populations.…”

Section: Introductionmentioning

confidence: 99%

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Population genetic structure and demographic history of <em>Aphanius fasciatus</em> (Cyprinodontidae: Cyprinodontiformes) from hypersaline habitats in the eastern Adriatic

Buj¹,

Miočić‐Stošić²,

Marčić³

et al. 2015

Sci. Mar.

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Summary:In order to investigate the phylogeography and population genetic structure of the South European toothcarp (Aphanius fasciatus), we analysed gene sequences of two mitochondrial markers (cytochrome b and mtDNA control region) in samples from eight localities along the eastern Adriatic coast and combined them with sequences from other Mediterranean localities. Since the South European toothcarp primarily inhabits hypersaline water bodies, it is a good model species for understanding patterns of colonization and dispersal of species adapted to variable conditions. The eastern Adriatic populations are separated into two groups of genetically related populations. The Northern group contains the populations from Sečovlje, Pag, Dinjiška, Nin and Pantan, whereas the Southern group contains the populations from Ston, Ulcinj and Narta. The majority of divergence events date back to the Pleistocene epoch and it is likely that sea level changes during glacial cycles played a significant role in shaping the recent genetic structure of this species. Our results imply pronounced intraspecific structuring of this species, whereas great environmental variations resulted in a smaller intrapopulational genetic diversity of A. fasciatus than seen in other Mediterranean fishes.Keywords: colonization; conservation priorities; divergence; evolution; genetic diversity; South European toothcarp.Estructura genética poblacional e historia demográfica de Aphanius fasciatus (Cyprinodontidae: Cyprinodontiformes) en hábitats hipersalinos del Adriático oriental Resumen: Con el objetivo de analizar la estructura genética y filogeografía de las poblaciones del fartet oriental (Aphanius fasciatus), se analizaron secuencias de genes de dos regiones mitocondriales (citocromo b y región de control del ADNmt) en individuos de ocho localidades a lo largo de la costa oriental del Adriático y de otras localidades mediterráneas. El fartet oriental habita principalmente masas de agua hipersalinas, y es una buena especie modelo para el estudio de los patrones de colonización y dispersión de especies adaptadas a condiciones variables. Los resultados de nuestro estudio muestran que las poblaciones del Adriático oriental se separan en dos grupos genéticamente relacionados. El grupo del Norte contiene las poblaciones de Sečovlje, Pag, Dinjiška, Nin y Pantan, mientras que el grupo del Sur contiene las poblaciones de Ston, Ulcinj y Narta. La mayoría de los eventos de divergencia pudiera ser que se remontaran a la época del Pleistoceno, y es probable que los cambios del nivel del mar durante los ciclos glaciales jugasen un papel importante en la estructura genética reciente de esta especie. Nuestros resultados revelan diferencias intraespecíficas pronunciadas en el fartet oriental, y por otro lado menor diversidad genética intrapoblacional como resultado de grandes variaciones ambientales en comparación con otros estudios de peces del Mediterráneo.Palabras clave: colonización; prioridades de conservación; divergencia; evolución; diversidad genética; fart...

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“…A. fasciatus therefore serves as model organism in many studies on genetic structures and levels of differentiation based on different methods, i.e. osteological characters (Tigano et al 1999, Ferrito et al 2003, genetic data (Hrbek and Meyer 2003, Triantafyllidis et al 2007, Pappalardo et al 2008, both osteological and genetic data , Tigano et al 2004, and otoliths (Reichenbacher et al 2007). These studies have revealed that A. fasciatus from Italy, Greece and Northern Tunisia is genetically structured by geographic isolation and that a clear link exists between genetic structures and the Pleistocene history of the Mediterranean Sea.…”

Section: Introductionmentioning

confidence: 99%

Inter-population differences in otolith morphology are genetically encoded in the killifish <i>Aphanius fasciatus</i> (Cyprinodontiformes)

Annabi¹,

Saïd²,

Reichenbacher³

2013

Sci. Mar.

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SUMMARY: Inter-population differences in otolith shape, morphology and chemistry have been used effectively as indicators for stock assessment or for recognizing environmental adaptation in fishes. However, the precise parameters that affect otolith morphology remain incompletely understood. Here we provide the first direct support for the hypothesis that inter-population differences in otolith morphology are genetically encoded. The study is based on otolith morphology and two mitochondrial markers (D-loop, 16S rRNA) of three natural populations of Aphanius fasciatus (Teleostei: Cyprinodontidae) from Southeast Tunisia. Otolith and genetic data yielded congruent tree topologies. Divergence of populations likely results from isolation events in the course of the Pleistocene sea level drops. We propose that otolith morphology is a valuable tool for resolving genetic diversity also within other teleost species, which may be important for ecosystem management and conservation of genetic diversity. As reconstructions of ancient teleost fish faunas are often solely based on fossil otoliths, our discoveries may also lead to a new approach to research in palaeontology.Keywords: Cyprinodontidae, phylogeography, gene flow, local adaptation, otolith morphometry, mitochondrial markers. RESUMEN: Las diferencias inter-pobLacionaLes en La morfoLogía deL otoLito de AphAnius fAsciAtus (cyprinodontiformes) se codifican genéticamente. -Las diferencias en la forma del otolito entre poblaciones, la morfología o la química se han utilizado con efectividad como indicadores para la gestión de poblaciones o para el reconocimiento de adaptaciones ambientales en peces. Sin embargo, los parámetros precisos que afectan la morfología del otolito permanecen sin ser entendidos completamente. Aquí nosotros presentamos la primera evidencia directa para la hipótesis de que las diferencias inter-poblacionales en la morfología del otolito están codificadas genéticamente. El estudio se basa en la morfología del otolito y dos marcadores mitocondriales (D-loop, 16S rRNA) de tres poblaciones naturales de Aphanius fasciatus (Teleostei: Cyprinodontidae) del sudeste de Tunez. Los datos de otolitos y genéticos ofrecen tres topologías congruentes. Aparentemente la divergencia entre poblaciones resulta de los procesos de aislamiento durante los descensos del nivel del mar en el Pleistoceno. Proponemos que la morfología del otolito es una herramienta muy valiosa para entender la diversidad genética también con otras especies de peces, que puede ser importante para la gestión de los ecosistemas y la conservación de la diversidad genética. Ya que las reconstrucciones de la fauna antigua de peces teleósteos se basan a menudo en otolitos fósiles, nuestro hallazgo puede también ser importante como nueva aproximación en las investigaciones palentológicas.

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Phylogeography and genetic structure of the Mediterranean killifish Aphanius fasciatus (Cyprinodontidae)

Cited by 32 publications

References 46 publications

Evaluation of environmental impact on natural populations of the Mediterranean killifish Aphanius fasciatus by quantitative RNA biomarkers

Evaluation of environmental impact on natural populations of the Mediterranean killifish Aphanius fasciatus by quantitative RNA biomarkers

Population genetic structure and demographic history of <em>Aphanius fasciatus</em> (Cyprinodontidae: Cyprinodontiformes) from hypersaline habitats in the eastern Adriatic

Inter-population differences in otolith morphology are genetically encoded in the killifish <i>Aphanius fasciatus</i> (Cyprinodontiformes)

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