Phylogeny of Five Species of Nusuttodinium gen. nov. (Dinophyceae), a Genus of Unarmoured Kleptoplastidic Dinoflagellates

Takano, Yoshihito; Yamaguchi, Haruyo; Inouye, Isao; Moestrup, Øjvind; Horiguchi, Takeo

doi:10.1016/j.protis.2014.09.001

Cited by 39 publications

(36 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…); Gymnodinium amphidinioides was transferred to Nusuttodinium because it is characterized by kleptoplastidy (Takano et al. ); Gymnodinium limneticum and Gymnodinium palustre were transferred to Spiniferodinium because they exhibit capsoid cells as predominant life stage (Kretschmann et al. ), and Gymnoxanthella was erected because it is a symbiont of polycystine radiolarians (Yuasa et al.…”

mentioning

confidence: 99%

Morphology, ultrastructure, and molecular phylogeny of Wangodinium sinense gen. et sp. nov. (Gymnodiniales, Dinophyceae) and revisiting of Gymnodinium dorsalisulcum and Gymnodinium impudicum

Luo¹,

Tang

et al. 2018

Journal of Phycology

View full text Add to dashboard Cite

The genus Gymnodinium includes many morphologically similar species, but molecular phylogenies show that it is polyphyletic. Eight strains of Gymnodinium impudicum, Gymnodinium dorsalisulcum and a novel Gymnodinium-like species from Chinese and Malaysian waters and the Mediterranean Sea were established. All of these strains were examined with light microscopy, scanning electron microscopy and transmission electron microscopy. SSU, LSU and internal transcribed spacers rDNA sequences were obtained. A new genus, Wangodinium, was erected to incorporate strains with a loop-shaped apical structure complex (ASC) comprising two rows of amphiesmal vesicles, here referred to as a new type of ASC. The chloroplasts of Wangodinium sinense are enveloped by two membranes. Pigment analysis shows that peridinin is the main accessory pigment in W. sinense. Wangodinium differs from other genera mainly in its unique ASC, and additionally differs from Gymnodinium in the absence of nuclear chambers, and from Lepidodinium in the absence of Chl b and nuclear chambers. New morphological information was provided for G. dorsalisulcum and G. impudicum, e.g., a short sulcal intrusion in G. dorsalisulcum; nuclear chambers in G. impudicum and G. dorsalisulcum; and a chloroplast enveloped by two membranes in G. impudicum. Molecular phylogeny was inferred using maximum likelihood and Bayesian inference with independent SSU and LSU rDNA sequences. Our results support the classification of Wangodinium within the Gymnodiniales sensu stricto clade and it is close to Lepidodinium. Our results also support the close relationship among G. dorsalisulcum, G. impudicum, and Barrufeta. Further research is needed to assign these Gymnodinium species to Barrufeta or to erect new genera.

show abstract

mentioning

confidence: 99%

Morphology, ultrastructure, and molecular phylogeny of Wangodinium sinense gen. et sp. nov. (Gymnodiniales, Dinophyceae) and revisiting of Gymnodinium dorsalisulcum and Gymnodinium impudicum

Luo¹,

Tang

et al. 2018

Journal of Phycology

View full text Add to dashboard Cite

show abstract

“…P. psammophilum and the species of the genus Nusuttodinium lack genuine chloroplasts (Takano et al. , Onuma et al. ).…”

Section: Discussionmentioning

confidence: 99%

Gymnoxanthella radiolariae gen. et sp. nov. (Dinophyceae), a dinoflagellate symbiont from solitary polycystine radiolarians

Yuasa

Horiguchi

Mayama

et al. 2016

Journal of Phycology

Self Cite

View full text Add to dashboard Cite

The symbiotic dinoflagellate Gymnoxanthella radiolariae T. Yuasa et T. Horiguchi gen. et sp. nov. isolated from polycystine radiolarians is described herein based on light, scanning and transmission electron microscopy as well as molecular phylogenetic analyses of SSU and LSU rDNA sequences. Motile cells of G. radiolariae were obtained in culture, and appeared to be unarmored. The cells were 9.1-11.4 μm long and 5.7-9.4 μm wide, and oval to elongate oval in the ventral view. They possessed an counterclockwise horseshoe-shaped apical groove, a nuclear envelope with vesicular chambers, cingulum displacement with one cingulum width, and the nuclear fibrous connective; all of these are characteristics of Gymnodinium sensu stricto (Gymnodinium s.s.). Molecular phylogenetic analyses also indicated that G. radiolariae belongs to the clade of Gymnodinium s.s. However, in our molecular phylogenetic trees, G. radiolariae was distantly related to Gymnodinium fuscum, the type species of Gymnodinium. Based on the consistent morphological, genetic, and ecological divergence of our species with the other genera and species of Gymnodinium s.s., we considered it justified to erect a new, separate genus and species G. radiolariae gen. et sp. nov. As for the peridinioid symbiont of radiolarians, Brandtodinium has been erected as a new genus instead of Zooxanthella, but the name Zooxanthella is still valid. Brandtodinium is a junior synonym of Zooxanthella. Our results suggest that at least two dinoflagellate symbiont species, peridinioid Zooxanthella nutricula and gymnodinioid G. radiolariae, exist in radiolarians, and that they may have been mixed and reported as "Z. nutricula" since the 19th century.

show abstract

“…Currently, 350 species are listed based on morphology (Mertens, Rengefors, Moestrup, & Ellegaard, 2012;Moestrup & Calado, 2018), and continuous efforts to revise them taxonomically have been made in the past years (Moestrup, Lindberg, & Daugbjerg, 2009;Craveiro, Pandeirada, Daugbjerg, Moestrup, & Calado, 2013;Takano, Yamaguchi, Inouye, Moestrup, & Horiguchi, 2014;Kretschmann, Filipowicz, Owsianny, Zinßmeister, & Gottschling, 2015;Kretschmann, Owsianny, Žerdoner Čalasan, & Gottschling, 2018, to mention only a few studies). Currently, 350 species are listed based on morphology (Mertens, Rengefors, Moestrup, & Ellegaard, 2012;Moestrup & Calado, 2018), and continuous efforts to revise them taxonomically have been made in the past years (Moestrup, Lindberg, & Daugbjerg, 2009;Craveiro, Pandeirada, Daugbjerg, Moestrup, & Calado, 2013;Takano, Yamaguchi, Inouye, Moestrup, & Horiguchi, 2014;Kretschmann, Filipowicz, Owsianny, Zinßmeister, & Gottschling, 2015;Kretschmann, Owsianny, Žerdoner Čalasan, & Gottschling, 2018, to mention only a few studies).…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic placement of environmental sequences using taxonomically reliable databases helps to rigorously assess dinophyte biodiversity in Bavarian lakes (Germany)

et al. 2019

View full text Add to dashboard Cite

1. Reliable determination of organisms is a prerequisite to explore their spatial and temporal occurrence and to study their evolution, ecology, and dispersal. In Europe, Bavaria (Germany) provides an excellent study system for research on the origin and diversification of freshwater organisms including dinophytes, due to the presence of extensive lake districts and ice age river valleys. Bavarian freshwater environments are ecologically diverse and range from deep nutrient-poor mountain lakes to shallow nutrient-rich lakes and ponds.2. We obtained amplicon sequence data (V4 region of small subunit-rRNA, c. 410 bp long) from environmental samples collected at 11 sites in Upper Bavaria. We found 186 operational taxonomic units (OTUs) associated with Dinophyceae that were further classified by means of a phylogenetic placement approach.3. The maximum likelihood tree inferred from a well-curated reference alignment comprised a systematically representative set of 251 dinophytes, covering the currently known molecular diversity and OTUs linked to type material if possible. Environmental OTUs were scattered across the reference tree, but accumulated mostly in freshwater lineages, with 79% of OTUs placed in either Apocalathium, Ceratium, or Peridinium, the most frequently encountered taxa in Bavaria based on morphology.4. Twenty-one Bavarian OTUs showed identical sequences to already known and vouchered accessions, two of which are linked to type material, namely Palatinus apiculatus and Theleodinium calcisporum. Particularly within Peridiniaceae, delimitation of Peridinium species was based on the intraspecific sequence variation. 5. Our approach indicates that high-throughput sequencing of environmental samples is effective for reliable determination of dinophyte species in Bavarian lakes.We further discuss the importance of well-curated reference databases that remain to be developed in the future. K E Y W O R D Samplicon sequencing, dinoflagellates, distribution, molecular phylogenetics, operational taxonomic unit

show abstract

Phylogeny of Five Species of Nusuttodinium gen. nov. (Dinophyceae), a Genus of Unarmoured Kleptoplastidic Dinoflagellates

Cited by 39 publications

References 52 publications

Morphology, ultrastructure, and molecular phylogeny of Wangodinium sinense gen. et sp. nov. (Gymnodiniales, Dinophyceae) and revisiting of Gymnodinium dorsalisulcum and Gymnodinium impudicum

Morphology, ultrastructure, and molecular phylogeny of Wangodinium sinense gen. et sp. nov. (Gymnodiniales, Dinophyceae) and revisiting of Gymnodinium dorsalisulcum and Gymnodinium impudicum

Gymnoxanthella radiolariae gen. et sp. nov. (Dinophyceae), a dinoflagellate symbiont from solitary polycystine radiolarians

Phylogenetic placement of environmental sequences using taxonomically reliable databases helps to rigorously assess dinophyte biodiversity in Bavarian lakes (Germany)

Contact Info

Product

Resources

About