Phylogenetic study and barcoding of the blood cockle, Tegillarca granosa, found on the west coast of peninsular Malaysia using the COI gene

Abstract

ABSTRACT. Blood cockles are among the most economically important brackish water invertebrates found in Malaysia. However, our knowledge of blood cockle phylogeny and systematics is rudimentary, especially for the species Tegillarca granosa. It is unclear, for instance, whether the cockles occurring on the west coast of peninsular Malaysia constitute a single species, or multiple, phylogenetically distinct species. We performed the first DNA molecular phylogenetic analysis of T. granosa to distinguish it from other related species found in other parts of the world and to create a DNA database for the species. An approximately 585-nucleotide fragment of the mitochondrial DNA (cytochrome oxidase I, COI) was sequenced for 150 individual cockles, representing 10 populations: three from the north, four from the central part and three from the southern part of peninsular Malaysia. Phylogenetic analyses of the resulting dataset yielded tree topologies that not only showed the relationship between T. granosa and its closest relatives but its position in the evolutionary tree. Three mitochondrial clades were evident, each containing an individual genus. Using the mutation rate of the COI gene, the divergence time between T. granosa and its closest related species was estimated to be 460 thousand years ago. This study provides a phylogenetic framework for this ecologically prominent and commercially important cockle species.

Production of specific pathogen free larvae from genetically characterized populations of Anadara tuberculosa (Bivalvia), for stock enhancement and aquaculture in the Peru Northeast Biosphere Reserve

“…The star-like shape of the TCS network and the shallow length of branches (the maximum distance between the ancestor haplotype and the most divergent haplotype corresponds to only seven nucleotides) would suggest a recent population expansion (Clement et al, 2000). As a point of comparison, haplotype diversity of A. tuberculosa from the NSMT was higher (36.7 vs 28.5%) than the genetic diversity reported for the same species extracted from Nariño region (Colombia) by Chamorro & Rosero (2016) or from Anadara granosa from Malaysia (14.7%) (Chee et al, 2011b). Figure 2.…”

Molecular analysis of the blood cockle, Tegillarca granosa (Linnaeus 1758) from Indonesia

“…studies on its phylogenetic status are limited in the literature especially the phylogenetics status in Indonesia. In the previous study reported by [18], reported only Malaysian T. granosa were phylogenetically and DNA Barcoding studied. [19], compared the phylogenetic status of Bivalvia, but also only include the T. granosa from South Korea.…”