Phylogenetic relationships of human-pathogenicCladosporium(Xylohypha) species inferred from partial LS rRNA sequences

Abstract

and ¶Unit6 de Biologie Cellulaire Marine, URA 6 71-CNRS Paris 6, Station Zoologique 06230, Villefranche sur Mer, FranceThe controversy about the appropriate taxonomic placement of agents of subcutaneous and systemic mycoses in either Cladosporium or Xylohypha, both genera characterized by conidia being produced in dry, acropetal chains, was addressed with partial sequencing of LS ribosomal RNA. Observation of catenate anamorphs in species of Capronia (Ascomycotina, Herpotrichiellaceae), a genus which also has anamorphs in Exophiala, suggested the possibility of a close interrelationship of all humanassociated taxa. To test this hypothesis, partial sequences of 43 strains of Cladosporium/Xylohypha were analysed. Human-pathogenic and saprophytic Cladosporium species were found to be phylogenetically distinct from each other and, on the basis of known teleomorph relationships, were considered to be anamorphs of Herpotrichiellaceae and Mycosphaerellaceae, respectively. They should therefore be classified in different anamorph-genera; Cladosporium being restricted to plantassociated species. A relatively large proportion of the Herpotrichiellaceae is presumed to be animal-associated. The black yeast genus Exophiala was also confirmed to be of herpotrichiellaceous relationship. The genus Xylohypha is unrelated.

Relationships of Scopulariopsis based on LSU rDNA sequences

“…A 367 bp partial sequence of the large subunit ribosomal RNA gene (LSU rDNA) was used to resolve subgeneric relationships in Cladophialophora and related fungi [10]. In our studies, sequences of this gene region also gave taxonomic results corresponding to those obtained with small subunit ribosomal DNA gene (SSU rDNA) in the genera Petriella and Pseudallescheria [11,12].…”

“…amplification and sequencing, the primers of Masclaux et al [10] were used. Our modifications of the isolation, primers and other details of DNA works have been described earlier [12].…”

Aspergillus vadensis, a new species of the group of black Aspergilli

“…Amplification of beta-tubulin gene was performed using the primers Bt2a and Bt2b (Glass and Donaldson 1995). Amplification of the two internal transcribed spacers (ITS 1 and ITS 2) including the 5.8 S rDNA was performed using the primers LS266 (Masclaux et al 1995) and V9G as proposed by de Hoog and Gerrits-van den Ende (1998). PCR was performed in a 50 ll reaction mixture using 10-20 ng genomic DNA in a GeneAmp PCR system 9700 (AB Applied Biosystems, Nieuwerkerk a/d IJssel, The Netherlands).…”

Four or More Species of Cladosporium Sympatrically Colonize Phragmites australis

“…3). All entries displayed belong to plant-associated Cladosporia since medically relevant Cladosporia were recently shown to be only distantly related to the former (Masclaux et al, 1995 large unresolved clade (59% bootstrap support) comprised strains assigned to C. cladosporioides, C. oxysporum, C. tenuissimum, C. herbarum, and C. macrocarpum. Bootstrap-supported branches lead to C. allii-cepae (leaf blotch on onion and leek), C. sphaerospermum, Cladosporium spp.…”